Cytochrome b Genetic Diversity and Maternal Origin of Chinese Domestic Donkey

Zhang, Yunsheng; Yang, Xueyi; Wang, Xiaobin; Zhang, Chunmei; Qin, Fang; Zhou, Zhen-Hui; Lan, Xianyong; Chen, Hong; Lei, Chuzhao

doi:10.1007/s10528-010-9345-0

Cited by 13 publications

(9 citation statements)

References 18 publications

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“…For He of the same single locus in comparable studies, Chinese donkeys were also similar to or slightly higher than European (Aranguren-Mendez et al 2001;Jordana et al 2001;Ivankovic et al 2002;Bordonaro et al 2012;Colli et al 2013) and American (Jordana et al 2015) donkeys. Those results were consistent with reports of previous mtDNA data on Chinese donkey breeds (Lei et al 2007;Zhang et al 2010).…”

Section: Discussionsupporting

confidence: 93%

“…Previous reports of Beja-Pereira et al (2004), Chen et al (2006), Lei et al (2007), Zhang et al (2010), and Perez-Pardal et al (2014) indicated a weak phylogeographic structure of indigenous donkey breeds by mtDNA studies. Using pedigree information, Gutierrez et al (2005) also suggested a weak population structure of indigenous Catalonian donkey breeds.…”

Section: Discussionmentioning

confidence: 86%

“…On the other hand, the origin of the species and evolutionary relationship between donkey breeds were analyzed using the mitochondrial DNA markers (Ivankovic et al 2002;Lopez Lopez et al 2005;Chen et al 2006;Zhang et al 2010;Han et al 2014;Perez-Pardal et al 2014), and these results hinted that the modern donkey (including Chinese donkey) has an African maternal origin. In this study, the genetic diversity and structure of five Chinese donkey breeds were assessed using 15 microsatellite markers in order to provide important implications for further conservation and utilization of Chinese donkeys.…”

mentioning

confidence: 99%

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Evaluation of genetic diversity and population structure of five Chinese indigenous donkey breeds using microsatellite markers

Di¹,

Liu²,

F³

et al. 2017

Czech J. Anim. Sci.

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China had the largest population of raising donkeys in the world, however the number of Chinese indigenous donkey decreased dramatically due to the increase of agriculture mechanization in the last century. The species has still been important in China because of its edible and medical value, therefore the survey on its genetic diversity in China is necessary for its conservation and utilization. In this study, 15 microsatellite markers were used to evaluate genetic diversity and population structure of five Chinese indigenous donkey breeds. The mean values of expected heterozygosity, allelic richness, and total number of alleles for all the tested Chinese donkeys were 0.70, 6.04, and 6.28 respectively, suggesting that the genetic diversity of Chinese indigenous donkeys is rich. The Bayesian analysis and principal component analysis plot yielded the same clustering result, which revealed that Guanzhong donkey was the most differentiated breed in all detected samples, and Jinnan (JN) and Guangling (GL) were genetically closed together. Additionally, our results indicated that the heterozygote deficit was severe in two Chinese indigenous donkey breeds (GL and JN), and it warned us that animal conservation activities on this species should be considered carefully in near future.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 86%

mentioning

confidence: 99%

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Evaluation of genetic diversity and population structure of five Chinese indigenous donkey breeds using microsatellite markers

Di¹,

Liu²,

F³

et al. 2017

Czech J. Anim. Sci.

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show abstract

“…; Zhang et al . ). However, mtDNA sequence variations reflect only the maternal contribution to the gene pool.…”

Section: Haplotypes Observed In 320 Donkeys and Their Distribution Frmentioning

confidence: 97%

Genetic diversity and paternal origin of domestic donkeys

et al. 2017

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Numerous studies have been conducted to investigate genetic diversity, origins and domestication of donkey using autosomal microsatellites and the mitochondrial genome, whereas the male-specific region of the Y chromosome of modern donkeys is largely uncharacterized. In the current study, 14 published equine Y chromosome-specific microsatellites (Y-STR) were investigated in 395 male donkey samples from China, Egypt, Spain and Peru using fluorescent labeled microsatellite markers. The results showed that seven Y-STRs-EcaYP9, EcaYM2, EcaYE2, EcaYE3, EcaYNO1, EcaYNO2 and EcaYNO4-were male specific and polymorphic, showing two to eight alleles in the donkeys studied. A total of 21 haplotypes corresponding to three haplogroups were identified, indicating three independent patrilines in domestic donkey. These markers are useful for the study the Y-chromosome diversity and population genetics of donkeys in Africa, Europe, South America and China.

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“…It is a classical gene in nearly all eukaryotes and most prokaryotes, whose full length is about 1 100-1 200 bp, and is conserved among many species (Gray, 1989;. To date, the Cytb gene has been widely used in many phylogenetic and population genetic studies (Zhao et al, 2008;Zhang et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Cloning, characterization, and expression of Cytochrome b (Cytb)—a key mitochondrial gene from Prorocentrum donghaiense

Zhao

Zhen

et al. 2011

Chin. J. Ocean. Limnol.

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Mitochondrial cytochrome b (Cytb), one of the few proteins encoded by the mitochondrial DNA, plays an important role in transferring electrons. As a mitochondrial gene, it has been widely used for phylogenetic analysis. Previously, a 949-bp fragment of the coding gene and mRNA editing were characterized from Prorocentrum donghaiense, which might prove useful for resolving P. donghaiense from closely related species. However, the full-length coding region has not been characterized. In this study, we used rapid amplifi cation of cDNA ends (RACE) to obtain full-length, 1 124 bp cDNA. Cytb transcript contained a standard initiation codon ATG, but did not have a recognizable stop codon. Homology comparison showed that the P. donghaiense Cytb had a high sequence identity to Cytb sequences from other dinofl agellate species. Phylogenetic analysis placed Cytb from P. donghaiense in the clade of dinofl agellates and it clustered together strongly with that from P. minimum. Based on the full-length sequence, we inferred 32 editing events at different positions, accounting for 2.93% of the Cytb gene. 34.4% (11) of the changes were A to G, 25% (8) were T to C, and 25% (8) were C to U, with smaller proportions of G to C and G to A edits (9.4% (3) and 6.2% (2), respectively). The expression level of the Cytb transcript was quantifi ed by real-time PCR with a TaqMan probe at different times during the whole growth phase. The average Cytb transcript was present at 39.27±7.46 copies of cDNA per cell during the whole growth cycle, and the expression of Cytb was relatively stable over the different phases. These results deepen our understanding of the structure and characteristics of Cytb in P. donghaiense, and confi rmed that Cytb in P. donghaiense is a candidate reference gene for studying the expression of other genes.

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Cytochrome b Genetic Diversity and Maternal Origin of Chinese Domestic Donkey

Cited by 13 publications

References 18 publications

Evaluation of genetic diversity and population structure of five Chinese indigenous donkey breeds using microsatellite markers

Evaluation of genetic diversity and population structure of five Chinese indigenous donkey breeds using microsatellite markers

Genetic diversity and paternal origin of domestic donkeys

Cloning, characterization, and expression of Cytochrome b (Cytb)—a key mitochondrial gene from Prorocentrum donghaiense

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