Cytochrome c interaction with hematite (α-Fe2O3) surfaces

Eggleston, Carrick M.; Khare, Nidhi; Lovelace, David M.

doi:10.1016/j.elspec.2005.06.006

Cited by 31 publications

(32 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is well known that the particle sizes and shapes of nanoscale hematite affect their properties and potential applications [75,76]. In the electrochemical sensors, up to now, it has been reported that mesoporous Fe 2 O 3 thin film electrodes catalyze glucose oxidation [77], tubelike a-Fe 2 O 3 shows unique sensing properties for nitric oxide [78], and mitochondrial cytochrome c (Mcc) sorbs strongly to hematite electrode from aqueous solution in a narrow pH range due to opposite charge on Mcc and hematite [79].…”

Section: Introductionmentioning

confidence: 99%

Electrochemistry and Adsorptive Stripping Voltammetric Determination of Amoxicillin on a Multiwalled Carbon Nanotubes Modified Glassy Carbon Electrode

Rezaei

Damiri

2009

Electroanalysis

View full text Add to dashboard Cite

The study of electrochemical behavior of amoxicillin (AMX), a b-lactam antibiotic, is described on a multiwalled carbon nanotubes (MWCNTs) modified electrode by electrochemical impedance spectroscopy (EIS) and adsorptive stripping voltammetry for sensitive determination of AMX in pharmaceutical and human urine samples within a wide pH range from 2.0 to 10.0. Also, studies by Fe 2 O 3 nanoparticles modified carbon paste electrode show that iron oxide impurities in the MWCNTs are not active sites for sensing of amoxicillin. Under optimized conditions, the oxidation peak has two linear dynamic ranges of 0.6 -8.0 and 10.0 -80.0 mM with a detection limit of 0.2 mM and a precision of < 4%.

show abstract

Section: Introductionmentioning

confidence: 99%

Electrochemistry and Adsorptive Stripping Voltammetric Determination of Amoxicillin on a Multiwalled Carbon Nanotubes Modified Glassy Carbon Electrode

Rezaei

Damiri

2009

Electroanalysis

View full text Add to dashboard Cite

show abstract

“…2. The dominant bands of the two products (at 472 and 555 cm -1 , 478 and 556 cm -1 ) are characteristic of crystalline a-Fe 2 O 3 [16]. The bands at 3,442 and 1,635 cm -1 belonged to the stretching vibration and bending vibration of OH, respectively.…”

Section: Methodsmentioning

confidence: 95%

Preparation of nanoparticles and hollow spheres of α-Fe2O3 and their properties

2011

View full text Add to dashboard Cite

Uniform nanoparticles and hollow microspheres of hematite (a-Fe 2 O 3 ) were obtained via a hydrothermal method by using iron (III) chloride as a precursor. The effects of reactant concentration, reaction time and temperature on the morphology of the samples were studied. The samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and superconducting quantum interference device magnetometer (SQUID) measurement. a-Fe 2 O 3 nanoparticles show a superparamagnetic behavior and the average size of the spherical particles was around 60 nm. However, hollow microspheres show a normal ferromagnetic behavior at room temperature with remanent magnetization and coercivity of 0.2482 emu/g and 2,516 Oe, respectively, and their average diameter was around 2 lm. The effects of reactant concentration and reaction temperature on the formation of the products were investigated. The experimental results reveal that the magnetic properties of hematite can be tuned by controlling the morphology.

show abstract

“…At hematite electrodes, native Mcc has a cathodic peak potential (E pc ) of about À100 mV and an anodic peak potential (E pa ) of about +240 mV vs Ag/AgCl (resulting in an E 0 0 of about +70 mV; Eggleston et al, 2005). This difference between reduction and oxidation waves is much larger than the 60 mV required of a fully reversible redox couple, implying that Mcc redox processes are not fully reversible at the hematite electrode.…”

Section: Electrochemistry Of MCC Using A-fe 2 O 3 (Hematite) Electrodesmentioning

confidence: 98%

“…Previous electrochemical work using hematite electrodes in solutions containing Mcc (Eggleston et al, 2005) produced voltammograms with redox waves giving an E 0 0 of about +70 mV vs. Ag/AgCl. This is the E 0 0 expected of native Mcc (Chen et al, 2002;Xu et al, 2004) and in agreement with earlier observations for Mcc on SnO 2 electrodes as well (Yeh and Kuwana, 1977).…”

Section: Electrochemistry Of MCC Using A-fe 2 O 3 (Hematite) Electrodesmentioning

confidence: 99%

Redox-linked conformation change and electron transfer between monoheme c-type cytochromes and oxides

Khare

Lovelace

Eggleston

et al. 2006

Geochimica et Cosmochimica Acta

Self Cite

View full text Add to dashboard Cite

The biochemistry of bacterial proteins involved in redox transformations of metals and minerals is, without dispute, an important area of research. Nevertheless, most studies on bacterial metal transformation have focused not on biochemistry but on genetics and genomics. The objective of this research is to better understand the role of conformation change in electron transfer from cytochromes to minerals, a process that underpins respiratory metal reduction by bacteria in nature and in bioremediation strategies, including reductive immobilization of radioactive contaminants. Our DOE-funded work is specifically focused on answering long-standing questions about the biochemical behavior of these very interesting proteins, and our findings thus far have already made impacts in the fields of environmental microbiology and biogeochemistry. Among the key findings from the project are 1) Successful large-scale production of biomass for protein isolation; 2) Purification of several c-type cytochromes for biochemical study; 3) Characterization of these proteins using spectrophotometric and electrochemical techniques; 4) Examination of protein conformational change and redox activity towards metal oxides using a small mass cytochrome c from Acidiphilium cryptum; 5) Proteomic characterization of A. cryptum biofilms; 6) Training of 2 undergraduate research assistants; 7) Publications and several meeting presentations.

show abstract

Cytochrome c interaction with hematite (α-Fe2O3) surfaces

Cited by 31 publications

References 44 publications

Electrochemistry and Adsorptive Stripping Voltammetric Determination of Amoxicillin on a Multiwalled Carbon Nanotubes Modified Glassy Carbon Electrode

Electrochemistry and Adsorptive Stripping Voltammetric Determination of Amoxicillin on a Multiwalled Carbon Nanotubes Modified Glassy Carbon Electrode

Preparation of nanoparticles and hollow spheres of α-Fe2O3 and their properties

Redox-linked conformation change and electron transfer between monoheme c-type cytochromes and oxides

Contact Info

Product

Resources

About