Cytochrome c/Multi-walled Carbon Nanotubes Modified Glassy Carbon Electrode for the Detection of Streptomycin in Pharmaceutical Samples

Abstract

A novel electrochemical glassy carbon electrode modified with multi-walled carbon nanotube, cytochrome c (Cyt c) and zinc oxide nanoparticles (ZnONPs) was fabricated to increase the sensitivity of electrode for the detection of streptomycin (STN) in certain pharmaceutical samples. Cyclic voltammetry (CV) and differential pulse voltammetry techniques were used for the electrochemical characterization of the electrode. Furthermore, the electrochemical biosensor construction phases were examined by using X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Under the optimal experimental conditions, the electrode offers a high selectivity and sensitivity signaling in the coexistence method of STN with linear concentration ranges from 0.02 to 2.2 μM. The detection limits (LOD) and limit of quantification (LOQ) were found to be 0.0028 µM and 0.0562 µM respectively. The fabricated sensing electrode has good stability, reproducibility and sensitivity towards STN in the pharmaceutical samples. Preliminary determination of binding sites within the specified grid box size, which covers both Cyt c and STN, were done by molecular docking analysis. Moreover, density functional theory (DFT) computations were performed to provide insightful information into optimized geometry of STN.

Electrochemical Behaviour of Selected Fenamate NSAIDs at PNAANI Modified Glassy Carbon Electrode

“…11 The above mentioned analytical parameters may be improved by using Chemically Modified Electrodes (CME) as sensors for specific electroactive analytes. 12 Recently such sensors are gaining interest in pharmaceutical analysis [16][17][18][19][20][21] and agricultural research. [22][23][24] Such electrochemical sensors are often prepared by depositing conducting polymers or their composites on the electrode surface.…”