Cytochrome P-450—Its Role in Oxygen Activation for Drug Metabolism

Energy metabolism, thyroid calorigenesis, and oxidative stress: functional and cytotoxic consequences

“…42 The antimycin-A-insensitive respiration is presumed to represent O 2 equivalents used in microsomal electron transfer involved in xenobiotic biotransformation. 43 Development of a hyperthyroid state involving higher rates of hepatic O 2 uptake results in the proliferation of the smooth endoplasmic reticulum, with higher activities of glucose-6-phosphatase, 40 NADPHcytochrome P-450 reductase, 40,[44][45][46] and NADPH oxidase, 38 the latter enzymatic activity representing the oxidase activity of cytochrome P-450 responsible for microsomal O 2…”

Effects of the Kupffer cell inactivator gadolinium chloride on rat liver oxygen uptake and content of mitochondrial cytochromes

“…GdCl Q (10 mg/kg) or saline (controls) was given via tail vein injection, and studies were performed at 0 (controls), 6,16,24, and 48 h after treatment under fasting conditions (24 h). All animals used received humane care according to the guidelines outlined in the Guide for the Care and Use of Laboratory Animals by the National Academy of Sciences (National Institutes of Health publication 86-23).…”

“…In fact, isolated liver mitochondria from GdCl Q -treated rats exhibit signi¢cant decreases in state 3 respiration and in the respiratory control ratio over control values, using glutamate plus malate as electron donors, without changes in ADP/ O ratios [14]. Considering that O P utilization by the hepatocyte is primarily due to mitochondrial activity over that associated with the microsomal fraction [16], the aim of the current study is to investigate the mechanism underlying the inhibitory e¡ect of GdCl Q on mitochondrial respiration, and the reversibility of the induced changes. To achieve this aim, the in£uence of GdCl Q on the content of rat liver mitochondrial cytochromes was studied.…”