Cytochrome P450 aromatase in grey mullet: cDNA and promoter isolation; brain, pituitary and ovarian expression during puberty

Developmental expression of steroidogenic factor-1, cyp19a1a and cyp19a1b from common carp (Cyprinus carpio)

“…However, the SF-1 binding sites were found only in the promoter of cyp19a1a and not that of cyp19a1b in some teleosts, such as zebrafish (Danio rerio), goldfish (Carassius auratus) and gray mullets (Mugil cephalus) (Tchoudakova et al, 2001;Tong and Chung, 2003;Nocillado et al, 2007). Thus, it is still unclear whether there is a cooperative mechanism of regulation of gonad differentiation by SF-1, cyp19a1a and cyp19a1b.…”

Local expression of CYP19A1 and CYP19A2 in developing and adult killifish (Fundulus heteroclitus)

“…Promoter analysis of fish CYP19A1s has identified cAMP-response elements, aryl hydrocarbon response elements, steroidogenic factor 1 (SF-1), TATA box, estrogen receptor recognition half sites, GATA-3, progesterone receptor response elements, Ad4BP binding motif and SRY/SOX motifs (Kazeto et al, 2001;Nocillado et al, 2007;Tanaka et al, 1995;Tchoudakova et al, 2001;Tong and Chung 2003). Using quantitative RT-PCR, unfertilized zebrafish eggs had similar levels of CYP19A1 expression as whole ovaries however these levels decreased during development until 48 hpf when CYP19A1 mRNA began to accumulate and peak at 72 hpf.…”

Expression pattern, cellular localization and promoter activity analysis of ovarian aromatase (Cyp19a1a) in protogynous hermaphrodite red-spotted grouper

“…The promoterless pGL3-basic vector (Promega) fused to a luciferase reporter gene was used to generate the reporter plasmids as described previously (Van Seuningen et al, 2000;Nocillado et al, 2006;Zhao et al, 2009). For EaCyp19a1a, we designed twelve pieces of forward primers according to the isolated 5 -flanking sequence, all containing KpnI site at 5 end, and one piece of reverse primer (Transfect-PR) derived from the sequence +105 bp downstream of the transcription start site, containing NheI site at its 5 end.…”

“…Cyp19a1a/b promoters were already isolated in some fishes and amphibians, such as medaka Oryzias latipes (Tanaka et al, 1995), goldfish Carassius auratus (Tchoudakova et al, 2001), zebrafish (Kazeto et al, 2001;Tong and Chung, 2003), Nile tilapia (Yoshiura et al, 2003;Chang et al, 2005), gobiid fish Trimma okinawae , European sea bass Dicentrarchus labrax L. (Galay-Burgos et al, 2006), grey mullet Mugil cephalus (Nocillado et al, 2006), Xenopus laevis (Akatsuka et al, 2005), and frog Rana rugosa (Oshima et al, 2006). And, some putative regulatory elements were also identified from some fish promoters (Yoshiura et al, 2003;Gardner et al, 2005;Nocillado et al, 2006).…”

“…And, some putative regulatory elements were also identified from some fish promoters (Yoshiura et al, 2003;Gardner et al, 2005;Nocillado et al, 2006). In comparison with the highly conserved response elements and binding sites for transcription factors among some mammalian aromatase promoters (Stocco, 2008), however, it remains unknown whether there is any common or conserved sequence in the fish aromatase promoter.…”