Cytochrome P450 Genes: Molecular Cloning and Overexpression in a Pyrethroid-Resistant Strain of Anopheles Minimus Mosquito

Rodpradit, Prinyada; Boonsuepsakul, Soamrutai; Chareonviriyaphap, Theeraphap; Bangs, Michael J.; Rongnoparut, Pornpimol

doi:10.2987/8756-971x(2005)21[71:cpgmca]2.0.co;2

Cited by 36 publications

(31 citation statements)

References 25 publications

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“…Cytochrome P450s have been linked to insecticide resistance in other major malaria vectors, including An. gambiae (21) and Anopheles minimus (22), as well as the major Dengue virus and Zika virus vector Aedes aegypti (17,23). In addition, many agricultural pests, including aphids (24), whiteflies (25), and plant hoppers (26), use the expression of multiple P450s to metabolize pesticides.…”

Section: Multiple Cytochrome P450s Are Driving Pyrethroid Resistance Inmentioning

confidence: 99%

Restriction to gene flow is associated with changes in the molecular basis of pyrethroid resistance in the malaria vector Anopheles funestus

Barnes

Irving

Chiumia

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Resistance to pyrethroids, the sole insecticide class recommended for treating bed nets, threatens the control of major malaria vectors, including Anopheles funestus. Effective management of resistance requires an understanding of the dynamics and mechanisms driving resistance. Here, using genome-wide transcription and genetic diversity analyses, we show that a shift in the molecular basis of pyrethroid resistance in southern African populations of this species is associated with a restricted gene flow. Across the most highly endemic and densely populated regions in Malawi, An. funestus is resistant to pyrethroids, carbamates, and organochlorides. Genome-wide microarray-based transcription analysis identified overexpression of cytochrome P450 genes as the main mechanism driving this resistance. The most up-regulated genes include cytochrome P450s (CYP) CYP6P9a, CYP6P9b and CYP6M7. However, a significant shift in the overexpression profile of these genes was detected across a south/north transect, with CYP6P9a and CYP6P9b more highly overexpressed in the southern resistance front and CYP6M7 predominant in the northern front. A genome-wide genetic structure analysis of southern African populations of An. funestus from Zambia, Malawi, and Mozambique revealed a restriction of gene flow between populations, in line with the geographical variation observed in the transcriptomic analysis. Genetic polymorphism analysis of the three key resistance genes, CYP6P9a, CYP6P9b, and CYP6M7, support barriers to gene flow that are shaping the underlying molecular basis of pyrethroid resistance across southern Africa. This barrier to gene flow is likely to impact the design and implementation of resistance management strategies in the region.

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Section: Multiple Cytochrome P450s Are Driving Pyrethroid Resistance Inmentioning

confidence: 99%

Restriction to gene flow is associated with changes in the molecular basis of pyrethroid resistance in the malaria vector Anopheles funestus

Barnes

Irving

Chiumia

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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“…In populations of A. aegypti from Latin America and Southeast Asia, the mutations Val1016Ile, Val1016Gly, Phe1534Cys and Asp1794Tyr, all in the IIS6 and IIIS6 segment, are correlated with insecticide resistance (BRENGUES et al 2003, CHANG et al 2009, HARRIS et al 2010, LINSS et al 2014. However, only one of these, a valine to isoleucine substitution at codon 1016, has been clearly linked to insecticide resistance; selection pressure under laboratory conditions, bioassays with adults, biochemical assays and molecular screening have confirmed this finding (RODPRADIT et al 2005, STRODE et al 2008, GARCÍA et al 2009, MARTINS et al 2009, LUMJUAN et al 2011, MARCOMBE et al 2012.…”

mentioning

confidence: 99%

Insecticide resistance and genetic variability in natural populations of Aedes (Stegomyia) aegypti (Diptera: Culicidae) from Colombia

Aguirre-Obando¹,

Bona²,

Duque³

et al. 2015

Zoologia (Curitiba)

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“…funestus and An. minimus (Brooke et al 1999, Vulule et al 1999, Hargreaves et al 2000, Rodpradit et al 2005, Etang et al 2007. While the esterases are associated mainly with resistance to organophosphates and carbamates, high levels of these enzymes have also been involved with resistance to permethrin in An.…”

mentioning

confidence: 99%

Mixed-function oxidases and esterases associated with cross-r esistance between DDT and lambda-cyhalothrin in Anopheles darlingi Root 1926 populations from Colombia

Fonseca-González

Quiñónes

McAllister

et al. 2009

Mem. Inst. Oswaldo Cruz

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In order to establish the insecticide susceptibility status for Enzyme levels related to insecticide resistance, including mixed function oxidases (MFO), non-specific esterases (NSE), glutathione S-transferases and modified acetylcholinesterase were evaluated in all populations and compared with a susceptible natural strain. Only mosquitoes from Amé-Beté presented significantly increased levels of both MFO and NSE, consistent with the low mortalities found in this population. The continued use of lambdacyhalothrin for An. darlingi control in this locality has resulted in a natural resistance to this insecticide. In addition, DDT resistance is still present in this population, although this insecticide has not been used in Colombia since 1992. Increased metabolism through MFO and NSE may be involved in cross-resistance between lambdacyhalothrin and DDT, although kdr-type nerve insensitivity cannot be discarded as a possible hypothesis. Additional research, including development of a kdr specific assay for An. darlingi should be conducted in future studies. Our data demonstrates the urgent need to develop local insecticide resistance management and surveillance programs throughout Colombia.Key words: insecticide resistance -bioassays -oxidases -esterases -Anopheles darlingi -Colombia Malaria is one of the major causes of morbidity and mortality worldwide. Nineteen countries in Latin America report active transmission of the disease, with one million clinical episodes annually. In addition, almost 250 million people are currently living in areas of risk for malarial infection (PAHO 2005). After Brazil, Colombia is the country with the highest index of malaria transmission in the Americas (WHO 2005a). Of all the cases that occurred during 2007, 27.5% were caused by Plasmodium falciparum. The zones of greatest transmission are located in the region of Bajo Cauca, Pacific Coast, the Valley of Sinú River and the region of Urabá (WHO 2005a, Sivigila 2007. In these areas, epidemic outbreaks are closely related with ecological imbalances and population displacement, resulted from the social situation in the country (WHO 2005b Although early diagnosis and treatment contributes to the prevention of malaria, control of vectors continues to be the most effective way of interrupting transmission in endemic areas. This measure of control is considered to be one of the four basic technical elements of the global strategy for malaria control (WHO 1993). Although vector control methods vary considerably in their applicability, costs and the reproducibility of results, there is a worldwide consensus for the use of indoor residual spraying (IRS) and insecticide treated nets (ITNs) with residual insecticides as major measures for the prevention of malaria. Both methods are effective in reducing the transmission of this disease through reducing the longevity of the vectors and decreasing human-vector contact (Lengeler & Sharp 2003).There are 45 Anopheles species documented in Colombia, but only seven are considered to be malaria vecto...

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Cytochrome P450 Genes: Molecular Cloning and Overexpression in a Pyrethroid-Resistant Strain of Anopheles Minimus Mosquito

Cited by 36 publications

References 25 publications

Restriction to gene flow is associated with changes in the molecular basis of pyrethroid resistance in the malaria vector Anopheles funestus

Restriction to gene flow is associated with changes in the molecular basis of pyrethroid resistance in the malaria vector Anopheles funestus

Insecticide resistance and genetic variability in natural populations of Aedes (Stegomyia) aegypti (Diptera: Culicidae) from Colombia

Mixed-function oxidases and esterases associated with cross-r esistance between DDT and lambda-cyhalothrin in Anopheles darlingi Root 1926 populations from Colombia

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