Cytochrome P450 (P450) isoforms expression, P450 concentration, monooxygenase activities, reactive oxygen species formation, lipid peroxidation, and glutathione content in wild catch carp and tench liver - influence of a two weeks exposure to phenobarbital

Klinger, W; Lupp, Amelie; Barth, A.; Karge, E.; Knels, Lilla; Kuhn, Urs Dieter; Müller, Dieter; Schiller, F.; Demme, U.; Lindström-Seppä, Pirjo; Hänninen, Osmo

doi:10.1016/s0940-2993(01)80008-7

Cited by 9 publications

(5 citation statements)

References 16 publications

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“…The reduced (GSH) and oxidized (GSSG) glutathione contents of the cells were determined according to Klinger et al . . In brief, cells of four wells (approx.…”

Section: Methodsmentioning

confidence: 99%

Effects of Narrow‐band IR‐A and of Water‐Filtered Infrared A on Fibroblasts

Knels

Valtink

Piazena

et al. 2016

Photochem & Photobiology

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Exposures of the skin with electromagnetic radiation of wavelengths between 670 nm and 1400 nm are often used as a general treatment to improve wound healing and reduce pain, for example, in chronic diabetic skin lesions. We investigated the effects of water-filtered infrared A (wIRA) and of narrow-band IR-A provided by a light-emitting diode LED (LED-IR-A) irradiation in vitro on 3T3 fibroblast cultures under defined conditions with and without glyoxal administration. Glyoxal triggers the formation of advanced glycation end products, thereby mimicking a diabetic metabolic state. Cell viability and apoptotic changes were determined by flow cytometry after vital staining with Annexin V, YO-PRO-1 and propidium iodide (PI), and by SubG1 assay. Mitochondrial function and oxidative stress were examined by vital staining for radical production, mitochondrial membrane potential (MMP) and the ratio of reduced-to-oxidized glutathione (GSH/GSSG). The metabolic state was monitored by a resazurin conversion assay. The numbers of apoptotic cells were reduced in cultures irradiated with wIRA or LED-IR-A. More mitochondria showed a well-polarized MMP after wIRA irradiation in glyoxal damaged cells. LED-IR-A treatment specifically restored the GSH/GSSG ratio. The immediate positive effects of wIRA and LED-IR-A observed in living cells, particularly on mitochondria, reflect the therapeutic benefits of wIRA and LED-IR-A.

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“…The reduced (GSH) and oxidized (GSSG) glutathione contents of the cells were determined according to Klinger et al . . In brief, cells of four wells (approx.…”

Section: Methodsmentioning

confidence: 99%

Effects of Narrow‐band IR‐A and of Water‐Filtered Infrared A on Fibroblasts

Knels

Valtink

Piazena

et al. 2016

Photochem & Photobiology

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“…For determining the oxidative state, treated cells from all six wells of a six‐well plate were harvested and combined. The reduced (GSH) and oxidized (GSSG) glutathione contents of the cells were measured as described by Klinger et al. (2001).…”

Section: Methodsmentioning

confidence: 99%

Blue light stress in retinal neuronal (R28) cells is dependent on wavelength range and irradiance

Knels

Valtink

Roehlecke

et al. 2011

European Journal of Neuroscience

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The aim of our study was to elucidate the role of wavelength and irradiance in blue light retinal damage. We investigated the impact of blue light emitted from light-emitting diode (LED) modules with peaks at either 411nm (half bandwidth 17nm) or 470nm (half bandwidth 25nm) at defined irradiances of 0.6, 1.5 and 4.5W/m(2) for 411nm and 4.5W/m(2) for 470nm on retinal neuronal (R28) cells in vitro. We observed a reduction in metabolic activity and transmembrane potential of mitochondria when cells were irradiated at 411nm at higher irradiances. Furthermore, production of mitochondrial superoxide radicals increased significantly when cells were irradiated with 411nm light at 4.5W/m(2) . In addition, such irradiation caused an activation of the antioxidative glutathion system. Using vital staining, flow cytometry and western blotting, we were able to show that apoptosis only took place when cells were exposed to 411nm blue light at higher irradiances; necrosis was not observed. Enhanced caspase-3 cleavage product levels confirmed that this effect was dependent on light irradiance. Significant alterations of the above-mentioned parameters were not observed when cells were irradiated with 471nm light despite a high irradiance of 4.5W/m(2) , indicating that the cytotoxic effect of blue light is highly dependent on wavelength. The observed phenomena in R28 cells at 411nm (4.5W/m(2) ) point to an apoptosis pathway elicited by direct mitochondrial damage and increased oxidative stress. Thus, light of 411nm should act via impairment of mitochondrial function by compromising the metabolic situation of these retinal neuronal cells.

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“…For instance, strong induction of Cyp1A, as reflected by increases in cyp1A mRNA and EROD activity, was also discovered in primary cultures of O. mykiss hepatocytes exposed to phenobarbital (Sadar et al , 1996). Similarly, phenobarbital exposure was able to induce EROD and cyp1A in the liver of carp Cyprinus carpio L. 1758 and tench Tinca tinca (L. 1758) (Klinger et al , 2001). Some carbamate and imidazole derivatives used as pesticides are also able to activate the Ahr in vitro (Boronat et al , 2007).…”

Section: Discussionmentioning

confidence: 99%

Differences in the induction of cyp1A and related genes in cultured rainbow trout Oncorhynchus mykiss. Additional considerations for the use of EROD activity as a biomarker

Valdehíta

Fernández‐Cruz

Torrent

et al. 2012

Journal of Fish Biology

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Two rainbow trout Oncorhynchus mykiss fish farms were repeatedly sampled in order to observe the variability of ethoxyresorufin-O-deethylase (EROD) activity and of related genes in the liver. Fish coming from fish farm A exhibited EROD levels that could be considered as basal according to the scientific literature, however, EROD activity in fish coming from fish farm B was significantly increased. This was accompanied by augmented aryl hydrocarbon receptor (ahr) and cytochrome P4501A (cyp1A) messenger RNA expression and reduced oestrogen receptor (er) and vitellogenin (vtg) transcription. Only sediment extracts from the entry channel of fish farm B induced EROD activity in O. mykiss cultured cells, however, this induction could not be explained by the levels of polyaromatic hydrocarbons (PAH) and polychlorinated biphenyls (PCB) measured in the sediments. The results of this study point out that O. mykiss cultured in fish farms could be used as sentinels for indication of pollution. In this particular work, however, no conclusive evidence has been found for a relationship between the presence of PAHs and PCBs and the observed EROD induction.

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Cytochrome P450 (P450) isoforms expression, P450 concentration, monooxygenase activities, reactive oxygen species formation, lipid peroxidation, and glutathione content in wild catch carp and tench liver - influence of a two weeks exposure to phenobarbital

Cited by 9 publications

References 16 publications

Effects of Narrow‐band IR‐A and of Water‐Filtered Infrared A on Fibroblasts

Effects of Narrow‐band IR‐A and of Water‐Filtered Infrared A on Fibroblasts

Blue light stress in retinal neuronal (R28) cells is dependent on wavelength range and irradiance

Differences in the induction of cyp1A and related genes in cultured rainbow trout Oncorhynchus mykiss. Additional considerations for the use of EROD activity as a biomarker

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