Cytocompatibility and biologic characteristics of synthetic scaffold materials of rabbit acellular vascular matrix combining with human-like collagen I

Abstract

Scaffold material provides a three-dimensional growing environment for seed cells in the research field of tissue engineering. In the present study, rabbit arterial blood vessel cells were chemically removed with trypsin and Triton X-100 to prepare rabbit acellular vascular matrix scaffold material. Observation by He&Masson staining revealed that no cellular components or nuclei existed in the vascular intima and media after decellularization. Human-like collagen I was combined with acellular vascular matrix by freeze-drying to prepare an acellular vascular matrix-0.25% human-like collagen I scaffold to compensate for the extracellular matrix loss during the decellularization process. We next performed a series of experiments to test the water absorbing quality, biomechanics, pressure resistance, cytotoxicity, and ultra-micro structure of the acellular vascular matrix composite material and natural rabbit artery and found that the acellular vascular matrix-0.25% human-like collagen I material behaved similarly to natural rabbit artery. In conclusion, the acellular vascular matrix-0.25% human-like collagen I composite material provides a new approach and lays the foundation for novel scaffold material research into tissue engineering of blood vessels.

<p>Resveratrol inhibits paclitaxel-induced neuropathic pain by the activation of PI3K/Akt and SIRT1/PGC1&alpha; pathway</p>

“…There are a lot of evidences to prove that the PI3K/Akt cascade participates in many cell functions including metabolism, proliferation, or growth 1,14. Therefore, the dysregulation of this cascade can lead to a variety of conditions, including cancer, diabetes, and neurologic diseases 17,25. Findings of the present study are consistent with the previous studies, which reported that the inhibition of PI3K dramatically alleviates inflammatory and neuropathic pain 19,20,30.…”

“…It has been reported that RES relieves neuropathic pain through the activation of spinal SIRT1 34. The phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway is one of the most important participants in modulating nociceptive information and is centrally sensitized in the microglial intracellular signaling cascades 25. RES has been found to protect neuronal cells from oxidative cytotoxicity via PI3K/Akt pathway 24.…”

Study of tissue engineered vascularised oral mucosa-like structures based on ACVM-0.25% HLC-I scaffold in vitro and in vivo

“…ACVM-0.25% -HLC-I scaffold is a tissue engineering composite scaffold material, which has the advantages of natural biological scaffold material and synthetic biodegradable polymer scaffold material. In other words, it provides three-dimensional structural space for cell growth and has certain biomechanical properties [14,16]. Our previous study [16] has demonstrated that ACVM-0.25% HLC-I has good compatibility with cells, low cytotoxicity, can promote cell adhesion, cell proliferation and cell migration, and has sufficient mechanical properties to maintain the tensile strength of the material.…”

“…An ACVM-0.25% HLC-I scaffold was prepared on the basis of previous studies [14,15]. The third-generation of HGFs with good growth condition was collected, digested at 0.25% trypsin (Sigma Biotechnology) 37 C for 5 min, and then centrifuged at 1000 rpm/min to remove supernatant and adjust cell concentration to 1 Â 10 6 cells/mL.…”

“…In this study, human gingival fibroblasts (HGFs), human gingival epithelial cells (HGECs) and vascular endothelial-like cells (VEC-like cells) [12] were used as seed cells in combination with ACVM-0.25% HLC-I [13][14][15][16] to construct tissueengineered vascularised oral mucosa-like structures in vitro and in vivo, and attempted to establish deep blood vessel connections through vascularisation, aiming at repairing tissue damage.…”