Cytocompatibility and Suitability of Protein-Based Biomaterials as Potential Candidates for Corneal Tissue Engineering

Abstract

The vision impairments suffered by millions of people worldwide and the shortage of corneal donors show the need of substitutes that mimic native tissue to promote cell growth and subsequent tissue regeneration. The current study focused on the in vitro assessment of protein-based biomaterials that could be a potential source for corneal scaffolds. Collagen, soy protein isolate (SPI), and gelatin films cross-linked with lactose or citric acid were prepared and physicochemical, transmittance, and degradation measurements were carried out. In vitro cytotoxicity, cell adhesion, and migration studies were performed with human corneal epithelial (HCE) cells and 3T3 fibroblasts for the films’ cytocompatibility assessment. Transmittance values met the cornea’s needs, and the degradation profile revealed a progressive biomaterials’ decomposition in enzymatic and hydrolytic assays. Cell viability at 72 h was above 70% when exposed to SPI and gelatin films. Live/dead assays and scanning electron microscopy (SEM) analysis demonstrated the adhesion of both cell types to the films, with a similar arrangement to that observed in controls. Besides, both cell lines were able to proliferate and migrate over the films. Without ruling out any material, the appropriate optical and biological properties shown by lactose-crosslinked gelatin film highlight its potential for corneal bioengineering.

Agar/gelatin hydro-film containing EGF and Aloe vera for effective wound healing

“…facilitated by the RGD motifs in gelatin, which resembled integrin mediated cell attachment. 16,48,49 It is worth to mention that although AV has shown to enhance cell attachment due to the ability of glucomannan to bind on b2-integrins, we did not observe any differences between G and GA hydro-films 16 Anti-inflammatory study. To determine the anti-inflammatory activity of the hydro-films, we followed the experimental design represented in Fig.…”

Cross-Linked Collagen-Based Scaffold: Anti-infective Potential, Hydrophilic, and Biocompatible

“…The availability of good cytocompatibility was decisive for whether the biomaterials could be used in clinical medicine . In this study.…”

“…The availability of good cytocompatibility was decisive for whether the biomaterials could be used in clinical medicine. 36 In this study. their cytocompatibility was tested by CCK-8 and CLSM observation using L929.…”

Removal of Malachite Green Micro-pollutant from Wastewater using Circular Economy based Agricultural Solid Wastes

“…The broad diffraction spectrum with a major semi-crystalline peaks was observed at 2θ = 20° for AP powder and at 2θ = 16.3° and 20 o for CH powder respectively. For both samples the broad spectrums at about 2θ = 20° indicates the materials are highly amorphous and porous in nature that is the characteristic of plant based natural polymers resulted from the diffusion scattering of biomaterial bers [23].…”