Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing

Liu, Tie-lian; Jiang, Miao; Sheng, Wei; Xie, Yufeng; Huang, Qiang; Shan, Yunbo; Yang, Jicheng

doi:10.1631/jzus.b0900163

Cited by 99 publications

(72 citation statements)

References 21 publications

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“…These biomaterials are processed in many ways to achieve natural-based wound dressings, templates for delivery of bioactive agents and scaffolds to support cellular growth. Fibrin-like haemostatic materials are useful for cleaning and treatment of full-thickness skin lesions [45], keratin-based matrices showed potential as chronic wound dressings because of their interaction with the proteolytic wound environment to facilitate the healing process [46], SF-containing films and sponges are being extensively investigated in animal models for both superficial and full-thickness skin defects healing [47][48][49], whereas electrospun nanofibres made entirely from a globular BSA are intended for suturing and acceleration of wound closure wound [50].…”

Section: Biopolymers As Dressing Materialsmentioning

confidence: 99%

Polymers in Wound Repair

Francesko

Fernandes

Rocasalbas

et al. 2014

Advanced Polymers in Medicine

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Efficient dermal wound care implies providing a healing environment at the site of injury. Current repair techniques, including polymeric dressings, are able to accelerate only the healing of epidermal and partial thickness acute wounds based on maintaining the area moist. However, these are not efficient in treatment of full-thickness and chronic wounds, which lack in inborn regenerative elements and are highly prone to infections. For this reason the research interest is nowadays shifted towards functional biomaterials to tackle severe skin deteriorations by providing a beneficiary for healing pro-active and pathogen-free environment. Recent advances in molecular biology and materials science together with better understanding of wound pathophysiology allowed for designing of new wound care approaches that rely on biochemical stimuli to promote wound closure. Biopolymers that couple intrinsic antimicrobial and wound repair properties with hydrophilicity appear as suitable dressing platforms. These can be further upgraded using various bio-entities (therapeutic molecules, cells) with the ability to address specific targets in the biochemical environment of wounds in order to stimulate the healing process. This chapter summarises the abundant experimental and clinical data on polymers in advanced wound dressings, scaffolds for dermal regeneration and platforms for drug delivery.

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Section: Biopolymers As Dressing Materialsmentioning

confidence: 99%

Polymers in Wound Repair

Francesko

Fernandes

Rocasalbas

et al. 2014

Advanced Polymers in Medicine

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“…Regenerated silk fibroin materials in the form of films, nano-fibrous membrane and woven textile have been employed as wound dressing for skin regeneration. Liu et al [13] have explored the feasibility of using regenerated silk fibroin membrane to construct artificial skin substitute for wound healing. They observed that silk fibroin film does not have an adverse influence on the growth and biofunction of fibroblast and vascular endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

Flexible silk fibroin films for wound dressing

et al. 2015

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In the present study, an effort has been made to create dextrose incorporated (5-15 % w/w) flexible silk fibroin films for wound dressing applications. The flexibility of silk fibroin films increases with increase in dextrose content. The elongation at break properties of dextrose modified silk fibroin (DMSF) films increases from 3.2 % to 40 % with increase in dextrose content. The glass transition temperature (T g ) of the films decreases from 176 o C to 155 o C with increase in dextrose content. This shows that dextrose is acting as plasticizer for silk fibroin films. The structural and morphological properties of dextrose modified silk films (DMSF) are characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) scanning electron microscopy (SEM) and atomic force microscopy (AFM). FTIR and XRD studies show that the dextrose content does not affect the crystalline structure of silk fibroin films. The surface roughness of the films also increases with increases in dextrose content in DMSF films. The addition of dextrose enhances the swelling and hydrophilicity of silk fibroin films. The adherence, proliferation and viability of L929 fibroblast cells cultured on DMSF films indicate that it has ability to support cell growth and proliferation as compared to SF film. The 15 % DMSF film showed significantly higher mass loss than SF film after 50 days of incubation in Protease XIV. Further, the data presented here constitute strong evidence that dextrose modified film has the great potential to be utilized as dermal wound dressing material.

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“…In fact, pectin improves osteoblasts adhesion and proliferation on porous polylactic-co-glycolic acid matrix (44), promotes murine fibroblast and human muscle cell growth (45), improves the biocompatibility of medical devices (46,47 (34) observed that the glycerol concentration of 30% w/w was necessary to obtain adequate silk fibroin films for fibroblast cell culture. Our results indicate that the concentration of 12% w/w guarantees the adipose-derived stem cell adhesion.…”

Section: Discussionmentioning

confidence: 99%

Formulation and Characterization of Silk Fibroin Films as a Scaffold for Adipose-Derived Stem Cells in Skin Tissue Engineering

Chlapanidas

Tosca

Faragò

et al. 2013

Int J Immunopathol Pharmacol

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Skin substitutes are epidermal, dermal or complete bilayered constructs, composed by natural or synthetic scaffolds and by adherent cells such as fibroblasts, keratinocytes or mesenchymal stem cells. Silk fibroin is a promising polymer to realize scaffolds, since it is biocompatible, biodegradable, and exhibits excellent mechanical properties in terms of tensile strength. Moreover, fibroin can be added of others components in order to modify the biomaterial properties for the purpose. The aim of this work is to prepare silk fibroin films for adipose-derived stem cell (ADSCs) culture as a novel feeder layer for skin tissue engineering. Pectin has been added to promote the protein conformational transition and construct strength, while glycerol as plasticizer, providing biomaterial flexibility. Eighteen formulations were prepared by casting method using fibroin, pectin (range 1-10% w/w), and glycerol (range 0-20% w/w); films were characterized by Fourier transform infrared spectroscopy and differential scanning calorimetry assay, to select the optimal composition. A stable fibroin conformation was obtained using 6% w/w pectin, and the best mechanical properties were obtained using 12% w/w glycerol. Films were sterilized, and human ADSCs were seeded and cultured for 15 days. Cells adhere to the support assuming a fibroblastic-like shape and reaching confluence. The ultrastructural analysis evidences typical active-cell features and adhesion structures that promote cell anchorage to the film, thus developing a multilayered cell structure. This construct could be advantageously employed in cutaneous wound healing or where the use of ADSCs scaffold is indicated either in human or veterinary field.The development of skin substitutes dates back to 1975, when Rheinwald and Green (1) cultured keratinocytes on a layer of lethally irradiated 3T3 murine fibroblasts. Currently, this method remains the most reliable and widely used for in vitro keratinocyte culture, but some concerns are emerging about their safety (2-5). To overcome these limits, Sugiyama et al. (6) proposed the use of human irradiated adipose-derived stem cells (ADSCs) as a feeder cell layer: results showed that the morphology of irradiated stem cells was similar to 3T3, and both cell lines (stem and 3T3 cells) expressed genes promoting keratinocyte proliferation. Other researchers also showed that non-irradiated ADSCs can boost epithelialization, angiogenesis (7), capillary density and granulation thickness of transplanted cell-enriched collagen sponges (8). Altman et al. (9) observed that ADSCs, once seeded on acellular dermal matrix and applied on murine wound, spontaneously differentiated along vascular endothelial, fibroblastic and epidermal epithelial lineages and significantly improved wound healing; moreover, cells localize at implantation site.Current cutaneous substitutes are epidermal, dermal or

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Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing

Cited by 99 publications

References 21 publications

Polymers in Wound Repair

Polymers in Wound Repair

Flexible silk fibroin films for wound dressing

Formulation and Characterization of Silk Fibroin Films as a Scaffold for Adipose-Derived Stem Cells in Skin Tissue Engineering

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