Cytocompatible and Antibacterial Properties of Chitosan-Siloxane Hybrid Spheres

Abstract

Microporous spheres in a hybrid system consisting of chitosan and γ-glycidoxypropyltrimethoxysilane (GPTMS) have advantages in a range of applications, e.g., as vehicles for cell transplantation and soft tissue defect filling materials, because of their excellent cytocompatibility with various cells. In this study, microporous chitosan-GPTMS spheres were prepared by dropping chitosan-GPTMS precursor sols, with or without a cerium chloride, into liquid nitrogen using a syringe pump. The droplets were then freeze dried to give the pores of size 10 to 50 μm. The cell culture tests showed that L929 fibroblast-like cells migrated into the micropores larger than 50 μm in diameter, whereas MG63 osteoblast-like cells proliferated well and covered the granule surfaces. The spheres with cerium chloride showed antibacterial properties against both gram-negative and gram-positive bacteria.

Injectable microfluidic hydrogel microspheres based on chitosan and poly(ethylene glycol) diacrylate (PEGDA) as chondrocyte carriers

“…Previous studies have demonstrated that the nanobrous microstructure would have a positive effect on cell adhesion. 23,[57][58][59] Comparing with the chitosan-siloxane microspheres and Chitin microspheres as described in the previous studies, 60,61 in this research these injectable microspheres were prepared by a doublenetwork hydrogel based on the combination of chitosan and PEGDA, which would not only provide the mechanical stability due to the photo-crosslinking of PEGDA but also show the ability to enhance cell adhesion due to physical-crosslinking of chitosan. In particular, many previous studies indicated that microspheres with a nanobrous surface were able to enhance cell adhesion.…”

Layer-by-Layer Deposited Hybrid Polymer Coatings Based on Polysaccharides and Zwitterionic Silanes with Marine Antifouling Properties

“…There are plenty of studies of silane hybrids with CH, − AA, − carrageenan, , as well as with cellulose . The studies were performed with a variety of silanes, such as (3-glycidoxypropyl)­trimethoxy silane (GPTMS) which carries an epoxy ring, ,, and (3-isocyanatopropyl)­triethoxy silane (IPTES) that has an isocyanate group . The obtained materials exhibit versatile functions and can be used for encapsulation of proteins, as tissue engineering materials, , as drug-delivery vehicles, and as antimicrobial materials. , In this work, two different silanes were chosen as multilayer cross-linking agents: one was triethoxymethyl silane (TEMS) (Figure e) and the other one was the zwitterionic, newly synthesized silane, 3-(1-(3-(triethoxysilyl)­propyl)-4,5-dihydro-1 H -imidazol-3-ium-3-yl)­propane-1-sulfonate (ISTES) (Figure f) bearing imidazole and sulfonate groups.…”

“…The studies were performed with a variety of silanes, such as (3-glycidoxypropyl)­trimethoxy silane (GPTMS) which carries an epoxy ring, ,, and (3-isocyanatopropyl)­triethoxy silane (IPTES) that has an isocyanate group . The obtained materials exhibit versatile functions and can be used for encapsulation of proteins, as tissue engineering materials, , as drug-delivery vehicles, and as antimicrobial materials. , In this work, two different silanes were chosen as multilayer cross-linking agents: one was triethoxymethyl silane (TEMS) (Figure e) and the other one was the zwitterionic, newly synthesized silane, 3-(1-(3-(triethoxysilyl)­propyl)-4,5-dihydro-1 H -imidazol-3-ium-3-yl)­propane-1-sulfonate (ISTES) (Figure f) bearing imidazole and sulfonate groups. Zwitterionic molecules are known for their antiadhesion properties; they have been used to design coatings to resist adsorption of proteins , and attachment of freshwater and marine fouling organisms, − which is usually attributed to their high degree of hydration. − Cross-linking of the multilayers with silanes was achieved through siloxane bond formation between the polysaccharides and silanol produced by silane hydrolysis (Figure ).…”

“…There are plenty of studies of silane hybrids with CH, 41−44 AA, 45−47 carrageenan, 48,49 as well as with cellulose. 50 The studies were performed with a variety of silanes, such as (3glycidoxypropyl)trimethoxy silane (GPTMS) which carries an epoxy ring, 42,51,52 and (3-isocyanatopropyl)triethoxy silane (IPTES) that has an isocyanate group. 53 The obtained materials exhibit versatile functions and can be used for encapsulation of proteins, 45 as tissue engineering materials, 42,50 as drug-delivery vehicles, 54 and as antimicrobial materials.…”

“…53 The obtained materials exhibit versatile functions and can be used for encapsulation of proteins, 45 as tissue engineering materials, 42,50 as drug-delivery vehicles, 54 and as antimicrobial materials. 44,52 In this work, two different silanes were chosen as multilayer cross-linking agents: one was triethoxymethyl silane (TEMS) (Figure 1e) and the other one was the zwitterionic, newly synthesized silane, 3- 1f) bearing imidazole and sulfonate groups. Zwitterionic molecules are known for their antiadhesion properties; they have been used to design coatings to resist adsorption of proteins 55,56 and attachment of freshwater and marine fouling organisms, 57−59 which is usually attributed to their high degree of hydration.…”