Cytocompatible and spontaneously forming phospholipid polymer hydrogels

Building cell-containing multilayered phospholipid polymer hydrogels for controlling the diffusion of a bioactive reagent

“…Actually, it was found that the cells embedded in the PMBV/PVA hydrogel were rounded and they could not spread or migrate in the hydrogel. 20,22,23,[29][30][31][32] Effects of PTX on the cell proliferation-cycle distribution Next, we demonstrated the applicability of our approach to control PTX diffusion within the hydrogel layers by changes in the thickness of the diffusion-controlling barrier. First, we determined the effects of PTX on the cell proliferation-cycle distribution in HeLa-Fucci cells at 12 h aer stimulation.…”

“…The boronic acid/diol complex can be dissociated by sugar solutions, such as sorbitol, which has a stronger affinity to boronic acids than that of the diol group, and thus degrades the PMBV/PVA hydrogel. 23,30,31 There are no matrix metalloproteinase (MMP) sensitive segments in the PMBV/PVA hydrogel, so the PMBV/PVA hydrogel cannot be destroyed by MMPs secreted by cells. Therefore, it is impossible for the cells to degrade the hydrogels and subsequently enhance the permeability of the hydrogel.…”

“…Actually, it was found that the cells embedded in the PMBV/PVA hydrogel were rounded and they could not spread or migrate in the hydrogel. 20,22,23,[29][30][31][32]…”

Recent Advances in Injectable Dual Crosslinking Hydrogels for Biomedical Applications

“…At present, the in-situ gelation of hydrogels can be divided into two classifications ground on different crosslinking mechanisms, spontaneous gelation and stimulus-responsive gelation. [39] Spontaneous Gelation: The characteristic of spontaneous gelation is that hydrogels can form spontaneously at the injection site after mixing two precursor solutions without any environmental stimulations. Therefore, the crosslinking mechanisms are usually a series of physical interactions and chemical reactions, [40,41] such as ion coordination, [42] dynamic covalent bonds, [43][44][45] and enzyme crosslinking.…”

“…At present, the in‐situ gelation of hydrogels can be divided into two classifications ground on different crosslinking mechanisms, spontaneous gelation and stimulus‐responsive gelation. [ 39 ]…”