Cytodiagnosis of metastatic amelanotic melanomas by fine-needle aspiration biopsy

Lai, Raymond; Redburn, Jim; Nguyen, Gia‐Khanh

doi:10.1002/(sici)1097-0142(19980425)84:2<92::aid-cncr4>3.0.co;2-f

Cited by 17 publications

(13 citation statements)

References 9 publications

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“…[3][4][5][6][7] The most frequently used melanocytic markers in clinical practice are S-100 protein and HMB-45. Monoclonal antibody to S-100 protein, a calcium binding F-hand protein originally isolated from the brain, is a sensitive marker that reacts with more than 90% of melanomas.…”

mentioning

confidence: 99%

Comparison of Five Antibodies as Markers in the Diagnosis of Melanoma in Cytologic Preparations

Sheffield

Yee

Dorvault³

et al. 2002

Am J Clin Pathol

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We determined the sensitivity and specificity of 3 novel antibodies (microphthalmia transcription factor [Mitf], Melan-A, and tyrosinase) as markers for melanoma in cytologic preparations and compared the results with those of commonly used markers (S-100 protein [S-100] and HMB-45). We stained 72 cell blocks from 40 patients with melanoma and 32 with nonmelanocytic malignant neoplasms with antibodies against S-100, HMB-45, Mitf, Melan-A, and tyrosinase. Histologic correlation was available in more than 95% of cases. Nuclear stainingfor Mitf and cytoplasmic stainingfor S-100, HMB-45, Melan-A, and tyrosinase in more than 10% of tumor cells was considered positive. All 3 novel markers demonstrated sensitivity superior to S-100 and HMB-45. HMB-45, Melan-A, and Mitf demonstrated specificities of 97%. S-100 protein and tyrosinase were less specific. Sensitivity and specificity for the combination Mitf+/Melan-A+ were 95% and 100%, respectively, whereas they were 80% and 100%, respectively, for S-100+/HMB-45+. Mitf Melan-A, and tyrosinase are sensitive markersfor epithelioid melanoma. Mitf and Melan-A seem more specific than S-100 and tyrosinase. An antibody panel consisting of Mitf and Melan-A is superior to a panel of S-100 and HMB-45 in the diagnosis of melanoma in cytologic specimens.

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confidence: 99%

Comparison of Five Antibodies as Markers in the Diagnosis of Melanoma in Cytologic Preparations

Sheffield

Yee

Dorvault³

et al. 2002

Am J Clin Pathol

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“…Additional immunocytochemical staining may be helpful in questionable cases, although this was not routinely applied in our studies. 29 We were able to demonstrate in a previous study that the additional application of immuncytochemical staining using anti-S 100 and anti-HMB 45 antibodies did not improve the sensitivity or specificity of our method. 30 Additional molecular-biologic techniques, such as tyrosinase reverse transcriptasepolymerase chain reaction from fine-needle aspirates (FNA-PCR), allowed for increased sensitivity, especially in the subset of cases with very small or necrotic lesions.…”

Section: Discussionmentioning

confidence: 72%

Ultrasound-guided fine-needle aspiration cytology in the early detection of melanoma metastases

Voit

Mayer²,

Proebstle³

et al. 2000

Cancer

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“…Definitive melanoma diagnosis may be difficult from cytological examinations performed on FNAC smears on account of the varied morphological appearances of melanoma on cytological preparations [10]. Well-trained cytologists who have sufficient experience with cytological smears are thus needed, although immunocytochemical staining may also be useful in questionable cases [31]. This degree of cytological expertise is not required for histopathological analysis with CNB.…”

Section: Discussionmentioning

confidence: 99%

Ultrasound-guided core needle biopsy of superficial lymph nodes

Bohelay¹,

Battistella

Pagès³

et al. 2015

Melanoma Research

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To investigate the diagnostic value of ultrasound-guided core needle biopsy (US-CNB) in suspected cases of lymph node metastasis from cutaneous melanoma. All patients with cutaneous melanoma followed in Saint-Louis Hospital between 2006 and 2010 who underwent US-CNB for suspicion of melanoma lymph node metastasis were reviewed retrospectively. Histopathological results of US-CNB samples were classified as melanoma, other malignancy, suspicious, inadequate, or benign. The diagnostic accuracy of US-CNB was assessed by comparison with two reference standards: histopathological examination of the radical lymph node dissection or, when this was not available, clinical and radiological follow-up. The data from 72 US-CNB were analyzed. Forty-four melanomas, 22 benign, three other malignancies, three inadequate samples, and no inconclusive specimens were diagnosed. Seventy-one US-CNB results were confirmed (98.6%). US-CNB achieved high sensitivity, specificity, and positive predictive value (respectively, 97.9, 100, and 100%). No adverse events were reported after the procedure. US-CNB provided a mean tissue volume of 16.7 mm per lymphadenopathy. US-CNB has diagnostic value similar to that of fine-needle aspiration cytology. It represents a reliable alternative method in melanoma lymph node metastasis to avoid surgery in patients who will not benefit from it. US-CNB provides relatively large samples of tissue suitable for comprehensive genomic analyses currently needed for research and personalized care of melanoma patients.

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Cytodiagnosis of metastatic amelanotic melanomas by fine-needle aspiration biopsy

Cited by 17 publications

References 9 publications

Comparison of Five Antibodies as Markers in the Diagnosis of Melanoma in Cytologic Preparations

Comparison of Five Antibodies as Markers in the Diagnosis of Melanoma in Cytologic Preparations

Ultrasound-guided fine-needle aspiration cytology in the early detection of melanoma metastases

Ultrasound-guided core needle biopsy of superficial lymph nodes

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