Cytodifferentiation of Protoplasts Isolated from a Stem Embryo System of Brassica napus to Tracheary Elements

Kohlenbach, Hans Willy; Körber, M.; Li, Liang-cai

doi:10.1016/s0044-328x(82)80203-1

Cited by 10 publications

(2 citation statements)

References 9 publications

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“…Single-celled tracheids were observed in cultures from both leaf and cotyledon protoplasts but only when the cell division efficiency was > 50%. Kohlenbach et al [9] have also reported that a low proportion ofprotoplasts from B. napus differentiated directly into tracheids.…”

Section: Resultsmentioning

confidence: 93%

Increased totipotency of protoplasts from Brassica oleracea plants previously regenerated in tissue culture

Robertson

Earle

Mutschler

1988

Plant Cell Tiss Organ Cult

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Factors affecting the division of cells derived from leaf and cotyledon protoplasts from Brassica oleracea L. var. italica ('Green Comet' hybrid broccoli) were examined to optimize conditions for plant regeneration and to determine whether there was a genetic basis for improved regeneration from protoplasts derived from plants previously regenerated from tissue cultures [15]. When leaf protoplasts from different plants grown from hybrid seed were isolated and cultured simultaneously, division efficiencies of 1-95% were obtained. Cells from some plants showed high division efficiencies in consecutive experiments while cells from other plants had consistently low division rates. More plants from hybrid seed gave high division efficiencies when cotyledon protoplasts were used. However, cotyledon or leafprotoplasts from selfed progeny of regenerated plants produced more vigorous calli and more shoots than protoplasts from hybrid seed. These results suggest that there may be a genetic component to the increased totipotency of Brassica oleracea protoplasts.

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Section: Resultsmentioning

confidence: 93%

Increased totipotency of protoplasts from Brassica oleracea plants previously regenerated in tissue culture

Robertson

Earle

Mutschler

1988

Plant Cell Tiss Organ Cult

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“…Other tissues of this species have also been used for protoplast isolation and include: cotyledons [10], hypocotyls [1,3], roots [17], and stem embryos [6]. Disadvantages of these sources, however, include limited yields, production of highly heterogenous protoplast populations, and the requirement for large numbers of seedlings thereby increasing the probability of microbial contamination.…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration from stem cortex protoplasts of Brassica napus

Klimaszewska¹,

Keller²

1987

Plant Cell Tiss Organ Cult

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Cell layer strips composed of the epidermis and 7-9 layers of subepidermal cells were isolated from the 3-4 terminal internodes of Brassica napus cv Westar plants at the early flowering stage. The strips were precultured for one day in modified liquid MS [1 l] medium and subsequently incubated for 17-18h in a 0.4M mannitol solution containing 1% Macerozyme and 1% Cellulase 'Onozuka' R-10. Protoplast yield was 2-2.8 x 10 6 per 1.0 g of tissue. Protoplasts were cultured at 1 x 105/ml in three different media: S I [13], B [12] and L [8l. The first cell divisions occurred after 2-8 days of culture at frequencies of 20-54%. The highest growth rate of colonies was obtained in L medium containing 0.4 M sucrose and 2% Ficoll. After 4 weeks, green calli, 1-2 mm in diameter were transferred onto B 5 12] medium with 3 mgl-~ zeatin, 1% sucrose, 0. ! M mannitol and 0.5 % agarose for shoot regeneration. Up to 20% of the calli regenerated shoots which subsequently were rooted and established in soil in the greenhouse.

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Vascular Cell Differentiation

Kuriyama

Fukuda

2018

Annual Plant Reviews Online

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The sections in this article areTracheary Element (TE) Differentiation as a Model of Cell–Cell ConnectionEarly Processes Induced by Cell SeparationFactors that RegulateTECell DifferentiationEffects of Tissue Organization on Cell DifferentiationCell Wall Components Characteristic ofTEand/or Vascular CellsThe Degradation ofTEPrimary Cell Walls and Pore FormationCo‐Regulation of Cell Wall Degradation andPCDConclusion

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Cytodifferentiation of Protoplasts Isolated from a Stem Embryo System of Brassica napus to Tracheary Elements

Cited by 10 publications

References 9 publications

Increased totipotency of protoplasts from Brassica oleracea plants previously regenerated in tissue culture

Increased totipotency of protoplasts from Brassica oleracea plants previously regenerated in tissue culture

Plant regeneration from stem cortex protoplasts of Brassica napus

Vascular Cell Differentiation

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