Cytogenetic analysis in human breast carcinoma. II. Seven cases in the triploid/tetraploid range investigated using direct preparations

Hill, Sally M.; Rodgers, Catherine S.; Hultén, Maj

doi:10.1016/0165-4608(87)90082-3

Cited by 51 publications

(26 citation statements)

References 17 publications

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“…Few reports on CG analysis of breast cancer exist in the literature (5,15,21,36). They have shown the frequent complexity of karyotypes, with variations concerning the involvement of individual chromosomes in the numerous abnormalities.…”

Section: Discussionmentioning

confidence: 99%

Relevance of DNA ploidy as a measure of genetic deviation: A comparison of flow cytometry and cytogenetics in 25 cases of human breast cancer

Remvikos¹,

Gerbault‐Seurreau²,

Vielh³

et al. 1988

Cytometry

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Twenty-five human breast cancers, surgically resected, were studied by cytogenetic analysis and DNA flow cytometry (FCM). The establishment of karyotypes showed that multiple cell populations probably were derived from a single ancestor clone, because common marker chromosomes always could be demonstrated. Differences of up to 30% were observed when the estimates of DNA content by the two methods were compared. A general tendency toward the acquisition of large marker chromosomes should be at the origin of this discordance, as the proportion Cytogenetic (CGj analysis has proved to be a powerful tool for studying chromosomal abnormalities in human malignancy (37,46). A number of primary events thus have been characterized, some of which are tumor-type specific. However, it is a long and painstaking method and even in expert hands, is successful only for a limited number of samples. DNA modal values first were estimated on interphase nuclei by static cytophotometry, which showed frequent deviations as compared with the content of normal diploid cells and which introduced the prognostic significance of this parameter (3,4,41). The development of several methods for obtaining DNA histograms from solid tumors by flow cytometry (FCM) has facilitated the study of a large number of samples (9,30,42,44). The application of DNA-FCM on archival material (18) has extended the possibilities by permitting retrospective studies with a long follow-up (10,17,19). However, results have shown considerable variability, often within a specific cancer, but also between the different tumor types. Furthermore, if aneuploidy has been generally recognized as indicative of a poor prognosis, it has been shown to have favorable incidence on outcome in childhood acute leukemia (26).Studies comparing CG and FCM results, on various tumor types have shown the existence of discordant cases on the basis of DNA content (2,32,43). We have of markers for each case correlated significantly with the magnitude of the difference. Parallel use of the two methods revealed the existence of tumors with DNA diploid FCM profiles and highly abnormal hypodiploid karyotypes (35-40 chromosomes), which may explain the limited value of DNA ploidy as an independent prognostic factor in breast cancer.Key terms: Human breast cancer, DNA ploidy, cytogenetics, prognostic value shown previously that quantitative measurements of DNA (chromosome counts and DNA indices) are highly correlated in colorectal cancers (34).We are reporting here our CG and FCM results for 25 cases of human breast cancer. Chromosome banding has been used systematically for karyotypic analysis to obtain more qualitative information at the genetic level, as compared with DNA histograms. Implications as to the possible value of DNA index in terms of prognosis are discussed. MATERIALS AND METHODSNinety-four surgical specimens of previously untreated breast cancer were sent by pathologists to the cytogenetics department, 32 of which (34%) produced sufficient analyzable metaphases (4-41). FCM DNA histog...

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Section: Discussionmentioning

confidence: 99%

Relevance of DNA ploidy as a measure of genetic deviation: A comparison of flow cytometry and cytogenetics in 25 cases of human breast cancer

Remvikos¹,

Gerbault‐Seurreau²,

Vielh³

et al. 1988

Cytometry

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“…[3][4][5] As a rule, direct preparation of breast cancer samples, if successful, has resulted in complex karyotypes, [3][4][5] whereas short-term cultures of tumor material have revealed less complex 6,7 or even normal karyotypes. 1,2,8 These findings led to the assumption that the cell populations most breast cancers are composed of are highly heterogeneous at the cytogenetic level and sometimes even polyclonal.…”

mentioning

confidence: 99%

Fluorescence <i>in Situ</i> Hybridization Studies on Breast Tumor Samples for Distinguishing Between Different Subsets of Breast Cancer

Leuschner

Staats²,

Gohla³

et al. 1996

Acta Cytologica

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“…Differences were detected in the karyotypic profile according to the cell culture method used. However, the chromosomes more frequently involved regardless of the methods used, were chromosomes 1, 6, 8, 1 1, 3, 7, 16, 17, and 18, which are reported in the majority of cytogenetic studies on breast tumors (TRENT 1985;HILL et al 1987;DUTRILLAUX et al 1990;HAINSWORTH and GARSON 1990;MITCHELL and SANTIBANEZ-KOREF 1990;BULLERDIEK et al 1993;Lu et al 1993;PANDIS et al 1993PANDIS et al , 1995THOMPSON et al 1993;TRENT et al 1993;ROHEN et al 1995;STEINARSD~TTIR et al 1995). In the present study, we found the nonrandom involvement of the same chromosomes as described above in addition to abnormalities involving chromosomes X, 19, 20, and 22.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic Evaluation of 20 Primary Breast Carcinomas

et al. 2004

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Chromosome analysis was performed on samples from 20 Brazilian patients with breast cancer. All the samples were from untreated patients who presented the clinical symptoms for months or years before surgical intervention. Six cases showed axillary lymph node metastases. Clonal chromosome abnormalities were detected in all cases. The numerical alterations most frequently observed involved the loss of chromosomes X, 19, 20, and 22 followed by gain of chromosomes 9 and 8. Among the structural anomalies observed, there was preferential involvement of chromosomes 11, 6, I , 7, 3, and 12, supporting previous reports that these chromosomes may harbour genes of importance in the development of breast tumors. Two cases with a family history of breast cancer had in common total or partial trisomy 1.

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Cytogenetic analysis in human breast carcinoma. II. Seven cases in the triploid/tetraploid range investigated using direct preparations

Cited by 51 publications

References 17 publications

Relevance of DNA ploidy as a measure of genetic deviation: A comparison of flow cytometry and cytogenetics in 25 cases of human breast cancer

Relevance of DNA ploidy as a measure of genetic deviation: A comparison of flow cytometry and cytogenetics in 25 cases of human breast cancer

Fluorescence <i>in Situ</i> Hybridization Studies on Breast Tumor Samples for Distinguishing Between Different Subsets of Breast Cancer

Cytogenetic Evaluation of 20 Primary Breast Carcinomas

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