Cytogenetic analysis in lymphocytes from workers occupationally exposed to low levels of ionizing radiation

Dias, Francisca L.; Antunes, Lusânia Maria Greggi; Rezende, Pâmela A.; Carvalho, Fernando E.S.; Silva, Carla M.D.; Matheus, Jaqueline M.; Junior, José Vitor de Oliveira; Lopes, Gesner Pereira; Pereira, Gilberto A.; Balarin, Marly Aparecida Spadotto

doi:10.1016/j.etap.2006.10.003

Cited by 25 publications

(18 citation statements)

References 34 publications

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“…In the entire studied population, men had a higher NPB frequency, as in another study on healthy volunteers (29), but the knowledge of the effect of confounding factor on NPBs frequencies is not as extensive as on MNi. Generally, the effects of smoking on DNA damage in individuals exposed to IR are still unclear, with opposite results (9,20,24,26,30,31). In IR exposed subjects, smoking habits were associated with increased MNi frequency, which is in agreement with previous studies (20,30), but there was a significantly decreased MNi frequency in the control group.…”

Section: Discussionsupporting

confidence: 82%

“…Generally, the effects of smoking on DNA damage in individuals exposed to IR are still unclear, with opposite results (9,20,24,26,30,31). In IR exposed subjects, smoking habits were associated with increased MNi frequency, which is in agreement with previous studies (20,30), but there was a significantly decreased MNi frequency in the control group. Interestingly, NB frequency was significantly decreased both in the IR exposed workers and in the overall population.…”

Section: Discussionsupporting

confidence: 82%

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Polymorphisms in DNA repair genes: link with biomarkers of the CBMN cytome assay in hospital workers chronically exposed to low doses of ionising radiation / Polimorfizmi u genima za popravak DNA: poveznica s biomarkerima mikronukleus-testa u medicinskih radnika kronično izloženih niskim dozama ionizirajućeg zračenja

Milić

Rozgaj

Kašuba

et al. 2015

Archives of Industrial Hygiene and Toxicology

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Individual sensitivity to ionising radiation (IR) is the result of interaction between exposure, DNA damage, and its repair, which is why polymorphisms in DNA repair genes could play an important role. We examined the association between DNA damage, expressed as micronuclei (MNi), nuclear buds (NBs), and nucleoplasmic bridges (NPBs) and single nucleotide polymorphisms in selected DNA repair genes (APE1, hOGG1, XRCC1, XRCC3, XPD, PARP1, MGMT genes; representative of the different DNA repair pathways operating in mammals) in 77 hospital workers chronically exposed to low doses of IR, and 70 matched controls. A significantly higher MNi frequency was found in the exposed group (16.2±10.4 vs. 11.5±9.4; P=0.003) and the effect appeared to be independent from the principal confounding factor. Exposed individuals with hOGG1, XRCC1, PARP1, and MGMT wild-type alleles or APEX1, as well as XPD (rs13181) heterozygous showed a significantly higher MNi frequency than controls with the same genotypes. Genetic polymorphism analysis and cytogenetic dosimetry have proven to be a powerful tool complementary to physical dosimetry in regular health surveillance programmes.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 82%

Polymorphisms in DNA repair genes: link with biomarkers of the CBMN cytome assay in hospital workers chronically exposed to low doses of ionising radiation / Polimorfizmi u genima za popravak DNA: poveznica s biomarkerima mikronukleus-testa u medicinskih radnika kronično izloženih niskim dozama ionizirajućeg zračenja

Milić

Rozgaj

Kašuba

et al. 2015

Archives of Industrial Hygiene and Toxicology

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“…At DNA level, IR exerts its genotoxic effect by the induction of singleand double-strand breaks, a-basic sites and oxidized bases. Moreover, the exposure to IR has been associated with gene mutations, amplification of genetic material, chromosomal rearrangements and increased frequencies of apoptosis, chromosomal aberrations (CAs), micronuclei and sister chromatid exchanges (SCEs) (Lazutka et al, 1999;Limoli et al, 2000;Engin et al, 2005;Dias et al, 2007;Sari-Minodier et al, 2007;Sakly et al, 2012a,b). These genotoxic effects of the radiations would result in each individual in the induction of a carcinogenic process (Breimer, 1988;Overbeek et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Increased frequency of chromosomal aberrations and sister chromatid exchanges in peripheral lymphocytes of radiology technicians chronically exposed to low levels of ionizing radiations

Santovito

Cervella

Delpero

2014

Environmental Toxicology and Pharmacology

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Chromosome aberrations (CAs) and sister chromatid exchanges (SCEs) frequencies were estimated in peripheral lymphocytes from 21 radiology technicians, and from 21 nonexposed control subjects. We exclusively considered individuals who neither smoke nor consume drugs or alcohol for a period of at least two years prior to the analysis. Significant differences were found between exposed and controls in terms of SCEs and CAs frequencies. Technicians showed a significant higher number of high-frequency individuals (HFIs) with respect to the control group. Nevertheless, the mean frequency of SCEs observed among technician HFIs did not significantly differ with respect to that observed among control HFIs. Vice versa, the nonHFIs belonging to technicians group showed a statistically higher difference in the SCEs/NSM value with respect to the non-HFIs belonging to control group. Since the differences in the SCEs frequencies between the two groups are due to non-HFIs, our results seem to indicate a general genotoxic effect of the IR, not affected by HFIs. Among technicians, the level of chromosome damage correlated neither with years of radiation exposure nor with the age of the subjects. Vice versa, in the control group, a positive correlation was found between the number of SCEs and age. In both samples the gender status did not influence the frequencies of CAs and SCEs. Our results suggest that chronic long-term exposure to low doses of ionizing radiation could increase the CAs and SCEs frequencies. This study reinforces the relevance of the biomonitoring of hospital workers chronically exposed to ionizing radiation.

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“…Valja naglasiti da toksičnost bleomicina između ostaloga ovisi o prisutnosti iona željeza koje u reduciranom obliku bleomicin treba za svoje aktiviranje (53). Iako izloženost X-zračenju pri snimanju pluća tri mjeseca prije izloženosti bleomicinu ne mijenja osjetljivost na bleomicin (54), izloženost ionizirajućem zračenju u radnim uvjetima tu osjetljivost ipak smanjuje, što upućuje na postojanje adaptivnog odgovora na djelovanje bleomicina (55)(56)(57)(58).…”

Section: Ionizirajuće Zračenjeunclassified

“…Dok neka istraživanja pokazuju da dnevni unos vitamina C i E smanjuje količinu oštećenja kromosoma (55), čak ovisno o dozi (60, 61), druga ne upućuju na bilo kakve promjene u odgovoru na "poticaj" bleomicinom (58). U ljudi oboljelih od raka vrata i glave koji još nisu primili terapiju, uočena je ovisnost između niskog unosa vitamina C i E i odgovora u testu osjetljivosti na mutagene te njihovu ulogu u procjeni rizika od oboljenja (63,64).…”

Section: Vitamini I Prehranaunclassified

Genome Instability and Bleomycin Sensitivity Test

Milić

2010

Archives of Industrial Hygiene and Toxicology

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Procjena individualne osjetljivosti na mutagene često je dio istraživanja u epidemiološkim studijama koje prate pojavnost zloćudnih bolesti u populacijama. Posljedica djelovanja mutagena u genomu izloženih osoba jest nastanak određene, manje ili veće, količine oštećenja, uvjetovane individualnim razlikama u osjetljivosti. Viša razina takve genomske nestabilnosti znači opasnost (rizik) od razvoja zloćudnih bolesti. Interindividualne razlike u odgovoru na mutagene obično se povezuju i s promijenjenom (većinom smanjenom) sposobnosti (kapacitetom) za popravak DNA. Citogenetičke studije su pokazale da je genom tumorskih stanica nestabilniji od normalnih, a time i skloniji akumuliranju oštećenja, bilo da je nestabilnost uzrokovana nasljeđem, izloženošću ili kombinacijom tih dvaju učinaka. U oboljelih ispitanika utvrđena je povećana učestalost kromatidnih i kromosomskih aberacija naspram normalne populacije te sklonost razvoju određenih vrsta neoplazija. U praćenju povezanosti promijenjenog odgovora i pojavnosti tumora služe nam različiti biomarkeri. Kao indirektni pokazatelji uspješnosti popravka DNA često se rabe testovi osjetljivosti na mutagene u kulturama limfocita periferne krvi. Jedan od takvih testova je i bleomicinski test. Radiomimetik i citostatik, a po strukturi glikopeptid, bleomicin se u stanici prevodi u aktivni oblik sposoban cijepati molekulu DNA što uzrokuje brojne jednolančane i dvolančane lomove. Kao jednostavna i jeftina metoda, zasniva se na utvrđivanju ukupnog broja jednolančanih lomova u kromosomima limfocita uzgajanih u staničnoj kulturi koji su u uvjetima in vitro tijekom kasne G 2 -faze staničnog ciklusa bili izloženi bleomicinu. Ovaj revijalni rad daje pregled utjecaja raznih faktora na rezultate samog testa i pokazuje njegovu široku primjenu u proučavanju genomske nestabilnosti koju najčešće uzrokuje kombinacija raznih faktora. Arh Hig Rada Toksikol 2010;61:257-266 KLJUČNE RIJEČI: DNA-popravak, jednolančani lomovi, kromatidni lomovi, polimorfi zam, translokacije, tumorske stanice Milić M. BLEOMYCIN SENSITIVITY TEST Bleomicin-testProcjena individualne osjetljivosti na mutagene često je dio epidemioloških istraživanja koja prate pojavnost zloćudnih bolesti u populacijama. Naime, kao posljedica djelovanja mutagena u genomu izloženih osoba nastaje određena, manja ili veća, količina oštećenja, koja je uvjetovana individualnim razlikama u osjetljivosti. Viša razina takve genomske nestabilnosti znači opasnost (rizik) od razvoja zloćudnih bolesti. Interindividualne razlike u odgovoru na mutagene obično se povezuju i s promijenjenom (većinom smanjenom) sposobnosti (kapacitetom) za popravak DNA. Citogenetičke studije su pokazale da je genom tumorskih stanica nestabilniji od normalnih (1-3), a time i skloniji akumuliranju oštećenja, bilo da je nestabilnost uzrokovana nasljeđem, izloženošću ili kombinacijom tih dvaju učinaka (4). Nasljedna kromosomska nestabilnost opisana je u raznim sindromima povezanim s lomovima kromosoma, kao što su Bloomov sindrom, Fanconijeva anemija, Ataxia telangiectasia i ...

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Cytogenetic analysis in lymphocytes from workers occupationally exposed to low levels of ionizing radiation

Cited by 25 publications

References 34 publications

Increased frequency of chromosomal aberrations and sister chromatid exchanges in peripheral lymphocytes of radiology technicians chronically exposed to low levels of ionizing radiations

Genome Instability and Bleomycin Sensitivity Test

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