Cytogenetic analysis in various tissues of pregnancy loss

Bennett, J. H.; O'Bringer, Angela; Williams, H James; Wenger, Sharon L.

doi:10.1097/01.gim.0000196824.98461.f0

Cited by 5 publications

(4 citation statements)

References 1 publication

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“…Our observations and those of others [5,7] demonstrate that the average culture success rate varies by tissue type with placental villi being the highest (>80%) and fetal parts being the lowest (<40%). Placental decidua almost always represents maternal tissue and is thus not an appropriate specimen type for study [7-10]. …”

Section: Introductionsupporting

confidence: 80%

“…Recent studies have demonstrated that the type of tissue received by a cytogenetics laboratory is critical for the success of cell growth in culture and the subsequent karyotype analysis [5,7]. Our observations and those of others [5,7] demonstrate that the average culture success rate varies by tissue type with placental villi being the highest (>80%) and fetal parts being the lowest (<40%). Placental decidua almost always represents maternal tissue and is thus not an appropriate specimen type for study [7-10].…”

Section: Introductionsupporting

confidence: 59%

“…We hypothesized that fetal parts from a chromosomally normal fetus tend to have a better chance to grow in culture and yield a chromosome result. As villi have the highest culture success rate, clinicians should be encouraged to submit placental villi whenever possible [5,7]. …”

Section: Discussionmentioning

confidence: 99%

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Abnormalities in spontaneous abortions detected by G-banding and chromosomal microarray analysis (CMA) at a national reference laboratory

et al. 2014

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BackgroundCytogenetic evaluation of products of conception (POC) for chromosomal abnormalities is central to determining the cause of pregnancy loss. We compared the test success rates in various specimen types and the frequencies of chromosomal abnormalities detected by G-banding analysis with those found by Oligo-SNP chromosomal microarray analysis (CMA). We evaluated the benefit of CMA testing in cases of failed culture growth.MethodsConventional cytogenetic results of 5457 consecutive POC specimens were reviewed and categorized as placental villi, fetal parts, and unspecified POC tissue. The CMA was performed on 268 cases. Of those, 32 cases had concurrent G-banding results. The remaining 236 cases included 107 cases with culture failure and 129 cases evaluated by CMA alone.ResultsThe overall POC culture success rate was 75%, with the lowest for fetal parts (37.4%) and the highest for placental villi (81%). The abnormality rate was 58% for placental villi, but only 25% for fetal parts. Of the abnormalities detected, the most common were aneuploidies, including trisomy 16, triploidy, monosomy X, trisomy 22, trisomy 21 and trisomy 15, while the least encountered aneuploidies were trisomy 1, trisomy 19 and monosomies (except monosomy 21). Overall, POC specimens studied by CMA were successful in 89.6% of cases and yielded a 44.6% abnormality rate.ConclusionsPlacental villi yielded higher rates of culture success and a higher percentage of abnormal karyotypes than did other specimen types. The Oligo-SNP CMA method has demonstrated a viable alternative to the G-banding method in view of its advantages in detection of submicroscopic genomic aberrations, shorter turnaround time due to elimination of time required for culture and a higher test success rate.

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Section: Introductionsupporting

confidence: 80%

Section: Introductionsupporting

confidence: 59%

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Abnormalities in spontaneous abortions detected by G-banding and chromosomal microarray analysis (CMA) at a national reference laboratory

et al. 2014

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“…A recent report reviewed karyotype success rates for 214 cases of second-and third-tri-mester spontaneous fetal losses and found growth rates for postnatal fetal tissue samples to be 60% compared to 95% for prenatal chorionic villus samples. These findings suggest that placental tissue/chorionic villi may thus provide a better and more consistent source of cells for cytogenetic analysis in cases of fetal loss [15] .…”

Section: Commentmentioning

confidence: 92%

Value of Amniocentesis versus Fetal Tissue for Cytogenetic Analysis in Cases of Fetal Demise

et al. 2009

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Objective: Use of fetal tissue for cytogenetic analysis in cases of second- and third-trimester fetal demise frequently results in unacceptably high failure rates. We reviewed our ongoing use of amniocentesis prior to uterine evacuation to determine if this provided a better source of cells for cytogenetic analysis. Methods: We compared cytogenetic results using fetal tissues obtained following uterine evacuation to our ongoing use of amniotic fluid cell obtained by transabdominal amniocentesis prior to uterine evacuation from 2003 to 2008. Results: In 49 of the 63 cases evaluated by fetal tissue biopsies performed after uterine evacuation, a karyotypic analysis was obtained (77.8%). Among the 38 cases evaluated by amniocentesis, an amniotic fluid sample and fetal cytogenetic results were obtained in all 38 (100%) cases. Conclusion: Our findings indicate that amniocentesis is a more reliable source of cytogenetic information than fetal tissue in cases of second- and third-trimester fetal demise.

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Current awareness in prenatal diagnosis

2006

Prenatal Diagnosis

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of prenatal diagnosis. Each bibliography is divided into 17 sections: 1 Books, Reviews & Symposia; 2 General Interest; 3 Normal Fetal Development; 4 Gametogenesis and Pre‐implantation Diagnosis; 5 First Trimester Diagnosis; 6 Second Trimester Diagnosis; 7 Fetal Diagnosis by Ultrasound and Other Imaging; 8 Maternal Screening; 9 Screening for Carriers of Genetic Abnormality; 10 Technological Developments; 11 Confined Placental Mosaicism and Uniparental Disomy; 12 Molecular Cytogenetics; 13 Fetal Cells in Maternal Circulation; 14 Fetal Therapy; 15 Psychosocial Aspects; 16 Epidemiology and Environmental Factors; 17 Developmental Pathology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted

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Cytogenetic analysis in various tissues of pregnancy loss

Cited by 5 publications

References 1 publication

Abnormalities in spontaneous abortions detected by G-banding and chromosomal microarray analysis (CMA) at a national reference laboratory

Abnormalities in spontaneous abortions detected by G-banding and chromosomal microarray analysis (CMA) at a national reference laboratory

Value of Amniocentesis versus Fetal Tissue for Cytogenetic Analysis in Cases of Fetal Demise

Current awareness in prenatal diagnosis

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