Cytogenetic analysis of interspecific hybrid Helianthus praecox × H. annuus, its parental forms, and two backcrosses

Yushkina, L. L.; Nesterova, E. V.; Кириченко, В. В.; Dolgova, T. A.; Popov, V. N.

doi:10.3103/s009545270901006x

Cited by 7 publications

(4 citation statements)

References 6 publications

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“…They probably result from the nuclei and micronuclei of the first meiotic division that undergo an equational division. Similar meiotic aberrations have been reported in many genera: Citrus (Del Bosco et al 1999), the interspecific hybrids of Nicotiana tabacum x N. glauca (Trojak-Goluch and Berbe c 2003), the intergeneric hybrids of Agrostis stolonifera x Polypogon monspeliensis (Zhao et al 2007), the interspecific hybrids of Helianthus praecox x H. annuus (Yushkina et al 2009), Begonia (Dewitte et al 2010) and the allotriploid of (Populus alba x P. glandulosa) x P. tomentosa (Wang et al 2010). They can be attributed to chromosome pairing problems due to their lack of homology and are the expression of the typical behaviour of non-paired or univalent chromosomes.…”

Section: Discussionmentioning

confidence: 80%

Pollen viability and meiotic behaviour in intraspecific hybrids of Hydrangea aspera subsp. aspera Kawakami group x subsp. sargentiana

Crespel

Morel

2014

Plant Breeding

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Pollen viability and male meiosis in intraspecific hybrids of Hydrangea aspera subsp. aspera Kawakami group (2n = 2x = 36) and subsp. sargentiana (2n = 2x = 34) were investigated. Although it had been assumed that they were sterile, pollen viability was observed; it varied from 2.5% to 12.1%. The production of gametes with different chromosome numbers was implied by the analysis of the dispersion of the diameter distribution of pollen grains. Analysis of male meiosis made it possible to identify the origins with two major categories of meiotic aberrations: abnormal chromosome distribution (early chromosome migration at metaphase, lagging chromosomes at anaphase, micronuclei at telophase), leading to the formation of unbalanced tetrads and/or ones with supernumerary microspores; and abnormal spindle orientation in metaphase II (tripolar, fused and parallel spindles), leading to the formation of dyads or triads. The mode of 2n pollen formation is of the First Division Restitution type. The high level of parental heterozygosity that is normally associated with them should facilitate the transfer of a polygenic trait in breeding programme.Key words: pollen diameter -abnormal chromosome distribution -disoriented metaphase II spindles -2n pollen grain -first division restitutionThe genus, Hydrangea, comprises 23 species (McClintock 1957), the majority originating in Asia. Generally, most of these species are poorly known at both biological and horticultural levels. The most horticulturally important species are H. macrophylla (Thunb.) Ser. and H. paniculata Sieb. that have been used as ornamental garden plants for a long time. However, other species such as H. aspera D. Don are also of horticultural interest, because of the colour of their inflorescences and plant shapes. This species contains four subspecies (H. aspera D. Don subsp. aspera Villosa and Kawakami groups, subsp. strigosa (Redher) E.M. McClint., subsp. robusta (Hook. f. & Thomson) E.M. McClint. and subsp. sargentiana (Redher) E.M. McClint.) and has already been characterized both genetically and morphologically.In-depth genetic characterization was based on cytogenetic observations such as measurement of the quantity of nuclear DNA and karyotyping (Cerbah et al. 2001, Mortreau et al. 2010, as well as intersimple sequence repeats (ISSR) molecular markers (Mortreau et al. 2003, Crespel et al. 2012. To further understand genetic relationships within the H. aspera species complex, hybridizations were carried out, notably between the subsp. aspera Kawakami group and subsp. sargentiana, the most distant at the cytogenetic level (Mortreau and Lambert, unpublished data). A viable progeny set of ten individuals was obtained.Based on descriptors recommended by the Union for the Protection of New Varieties of Plants (UPOV) for examining distinctiveness, homogeneity and stability (UPOV, 1991), a first qualitative morphological characterization of H. aspera was carried out by Bertrand et al. (2007). This characterization mainly concerned foliage and inflorescen...

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Section: Discussionmentioning

confidence: 80%

Pollen viability and meiotic behaviour in intraspecific hybrids of Hydrangea aspera subsp. aspera Kawakami group x subsp. sargentiana

Crespel

Morel

2014

Plant Breeding

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“…A significant genotype effect was observed for the production of 2n pollen grains, with Hw20 being a very low producer and the other genotypes being low-level ones, with a production ranging from 0.3% to 1.3%. The production of 2n gametes was frequently observed in interspecific hybrids of many other genera (Ramanna and Jacobsen 2003); these interspecific hybrids exhibit abnormal or absent chromosome pairing and the presence of univalent, lagging chromosomes and chromosome bridges (Del Bosco et al 1999, Trojak-Goluch and Berbe c 2003, Yushkina et al 2009, Crespel and Morel 2014. A genotype effect was also observed for Lotus tenuis by Negri and Lemmi (1998) and for Brassica interspecific hybrids by Mason et al (2011).…”

Section: Discussionmentioning

confidence: 94%

“…, Trojak‐Goluch and Berbeć , Yushkina et al. , Crespel and Morel ). A genotype effect was also observed for Lotus tenuis by Negri and Lemmi () and for Brassica interspecific hybrids by Mason et al.…”

Section: Discussionmentioning

confidence: 98%

Effect of high temperature on the production of 2n pollen grains in diploid roses and obtaining tetraploids via unilateral polyploidization

et al. 2015

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To integrate the gene pool of a wild species (primarily diploid) into a cultivated pool (primarily tetraploid), a crossing between a dihaploid cultivated rose and a hybrid of Rosa wichurana allowed to obtain interspecific diploid hybrids that produced 2n pollen grains. A return to a tetraploid level sought by breeders can then be considered using sexual polyploidization, obtained by crossing a tetraploid cultivated rose with these hybrids. Application of a high-temperature regime led to a small but significant increase in the percentage of 2n pollen grains in these hybrids of up to 4.6%. This result was obtained by applying high temperatures close to 32°C during the day to plants cultivated in a glasshouse during recurrent cycles of bloom. Crosses were made between an unreleased tetraploid hybrid tea rose, as a female, and the diploid hybrid that produces the most 2n pollen grains. Tetraploid (42.1%) and triploid (57.9%) offspring were obtained. The use of these 2n pollen grains of the first division restitution type should facilitate the introgression of complex traits of interest. Key words: 2n gametesRose is the most economically important ornamental crop worldwide because of its popularity as a garden, landscape and potted plant, and as cut flower (Gudin 2000). The genus Rosa subgenus Rosa comprises ten sections and more than 200 species (Wissemann 2003, Foug ere-Danezan et al. 2015. Ma€ ıa and Venard (1976) and Berninger (1992) showed that <10 species belonging to three sections are at the origin of roses (Rosa hybrida L.) cultivated today. Many cultivated roses are tetraploid (2n = 4x = 28), whereas many wild species are generally diploid (2n = 2x = 14).Rose breeders have attempted to enrich the variability of the gene pool of cultivated roses by introducing germplasm of new diploid wild species. Many interspecific hybridizations between tetraploid rose cultivars and wild diploid species were carried out, but produced triploids that were generally sterile, making it very difficult to generate additional generations (Wylie 1955). To integrate the variability of diploid species into the gene pool of cultivated roses, a haploidization programme of cultivated roses was developed in the 1990s by Meynet et al. (1994) that consisted of reducing the ploidy level of cultivated rose from tetraploid to diploid. This programme involved three steps: (i) producing dihaploids (diploid) from tetraploid rose cultivars, (ii) creating progeny of interspecific diploid hybrids developed by crossing dihaploid cultivated rose cultivars with wild diploid species and (iii) returning to the tetraploid level (ploidy level most frequently encountered in the cultivated rose) (Crespel and Meynet 2003).Only the first two steps of this programme were carried out. Dihaploids of tetraploid rose cultivars were obtained by parthenogenesis in situ induced by irradiated pollen and immature embryo rescue techniques in vitro (Meynet et al. 1994). The study of their gametogenesis revealed different meiotic anomalies such as abnormal spind...

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“…In earlier studies, fewer than expected seed sets were reported in F 1 interspecies hybrids between cultivated sunflower and the wild H . praecox subspecies due to the meiotic chromosomal aberrations [48,49]. Although our crossing program began with five highly BSR-resistant H .…”

Section: Discussionmentioning

confidence: 99%

Introgression and monitoring of wild Helianthus praecox alien segments associated with Sclerotinia basal stalk rot resistance in sunflower using genotyping-by-sequencing

et al. 2019

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Sclerotinia basal stalk rot (BSR) and downy mildew are major diseases of sunflowers worldwide. Breeding for BSR resistance traditionally relies upon cultivated sunflower germplasm that has only partial resistance thus lacking an effective resistance against the pathogen. In this study, we report the transfer of BSR resistance from sunflower wild species, Helianthus praecox , into cultivated sunflower and molecular assessment of the introgressed segments potentially associated with BSR resistance using the genotyping-by-sequencing (GBS) approach. Eight highly BSR-resistant H . praecox introgression lines (ILs), H.pra 1 to H.pra 8, were developed. The mean BSR disease incidence (DI) for H.pra 1 to H.pra 8 across environments for four years ranged from 1.2 to 11.1%, while DI of Cargill 270 (susceptible check), HA 89 (recurrent parent), HA 441 and Croplan 305 (resistant checks) was 36.1, 31.0, 19.5, and 11.6%, respectively. Molecular assessment using GBS detected the presence of H . praecox chromosome segments in chromosomes 1, 8, 10, 11, and 14 of the ILs. Both shared and unique polymorphic SNP loci were detected throughout the entire genomes of the ILs, suggesting the successful transfer of common and novel introgression regions that are potentially associated with BSR resistance. Downy mildew (DM) disease screening and molecular tests revealed that a DM resistance gene, Pl 17 , derived from one of the inbred parent HA 458 was present in four ILs. Introgression germplasms possessing resistance to both Sclerotinia BSR and DM will extend the useful diversity of the primary gene pool in the fight against two destructive sunflower diseases.

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Cytogenetic analysis of interspecific hybrid Helianthus praecox × H. annuus, its parental forms, and two backcrosses

Cited by 7 publications

References 6 publications

Pollen viability and meiotic behaviour in intraspecific hybrids of Hydrangea aspera subsp. aspera Kawakami group x subsp. sargentiana

Pollen viability and meiotic behaviour in intraspecific hybrids of Hydrangea aspera subsp. aspera Kawakami group x subsp. sargentiana

Effect of high temperature on the production of 2n pollen grains in diploid roses and obtaining tetraploids via unilateral polyploidization

Introgression and monitoring of wild Helianthus praecox alien segments associated with Sclerotinia basal stalk rot resistance in sunflower using genotyping-by-sequencing

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