Cytogenetic and genotoxic effects of zinc oxide nanoparticles on root cells of Allium cepa

Kumari, Mamta; Khan, S. Sudheer; Pakrashi, Sunandan; Mukherjee, Amitava; Chandrasekaran, Natarajan

doi:10.1016/j.jhazmat.2011.03.095

Cited by 353 publications

(187 citation statements)

References 39 publications

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“…Similar chromotoxic effects of NPs were also reported by Kumari et al [8] and Panda et al [9] Similar study on root cells of Allium cepa reported that ZnO NPs have cytotoxic and genotoxic effects, including lipid peroxidation, mitotic index reduction and increase of micronuclei and chromosomal aberration indexes. [8] Li et al [10] and Chen et al [11] while working on mammalian cell lines, demonstrated that the NPs penetrated subcellular structures such as mitochondria and nucleus causing uncoupling of respiration and increased oxidative stress.…”

Section: Discussionsupporting

confidence: 84%

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Interaction of cobalt nanoparticles with plants: a cytogenetical aspect

Srivastava

2014

Journal of Experimental Nanoscience

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In vivo cytogenetical assay in root meristems has been carried out to study the effect of cobalt and cobalt oxide nanos on active mitotic index and chromosomal aberrations of green manure crop, Sesbania pea. Nanoparticles are nanosize particles and can enter freely into the cells and can interfere in cell's normal function. Studies to justify its probable after-effects on living beings are scarce. So it is important to study its effect on living beings. For this purpose, seeds of Sesbania cannabina variety ND-1 were soaked in distilled water for 14 hours and after soaking, the seeds were treated with three different treatments, namely, nanocobalt, nano-cobalt oxide and ethanol for three hours. The treated root meristems exhibited various types of chromosomal aberrations such as metaphasic plate distortion, unorientation at metaphase, breaking of chromosomes, fragmentation, spindle dysfunctioning, stickiness, scattering, precocious movement at metaphase and bridge, unequal separation, multiple bridge, fragmentation, scattering, laggard and unorientation at anaphase, etc.

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Section: Discussionsupporting

confidence: 84%

“…[8] Li et al [10] and Chen et al [11] while working on mammalian cell lines, demonstrated that the NPs penetrated subcellular structures such as mitochondria and nucleus causing uncoupling of respiration and increased oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Interaction of cobalt nanoparticles with plants: a cytogenetical aspect

Srivastava

2014

Journal of Experimental Nanoscience

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“…This decrease might be attributed to increases in oxidative stress depending on the dose application. Previous studies showed that AgNPs have genotoxic effects in plant cells due to the induced oxidative stress or generation of ROS (Kumari et al, 2011). Panda et al (2011) also demonstrated that AgNPs induced cell death and DNA damage via generation of ROS.…”

Section: Discussionmentioning

confidence: 98%

Silver nanoparticles induced genotoxicity and oxidative stress in tomato plants

Çekiç¹,

Ekinci²,

Inal³

et al. 2017

Turk J Biol

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IntroductionSilver nanoparticles (AgNPs) are widely used among other nanoparticles in many industries within a wide range of consumer products because of their antibacterial and biocidal properties (Thuesombat et al., 2014). In recent years, the significant increase in the consumption of nanoparticles has caused environmental, health, and safety concerns regarding their potential effects (Ma et al., 2010;Pokhrel and Dubey, 2013). Nanoparticles could uncertainly spread to the environment. However, the interaction between AgNPs and plant systems is still not well known (Patlolla et al., 2012;Song et al., 2013).AgNPs are known to be absorbed by plants and could interact with intracellular parts causing water imbalances, cell damage, and decreases in photosynthesis (Kumari et al., 2009;Qian et al., 2013). They are also reported to have genotoxic effects on plant cells, inducing chromosomal aberrations and micronucleus induction (Patlolla et al., 2012). However, the impacts of nanoparticles on plants can vary according to the nanoparticle concentration, size, chemical properties, and plant species (Ma et al., 2010;Thuesombat et al., 2014).Nanotoxicity could lead to oxidative stress and previous studies indicate that AgNPs could induce toxicity due to their effect on reactive oxygen species (ROS) formation (Qian et al., 2013;McShan et al., 2014). The imbalance of ROS production and antioxidant activity can cause oxidative damage, and plants cope with this oxidative damage by their antioxidant defense mechanism (Saed-Moucheshi et al., 2014). Previously, studies on the genotoxicity of nanoparticles have used cell viability, chromosome aberration, or micronucleus assays to identify the genotoxic effect, and comet analysis for detecting the DNA damage in different plant species (Kumari et al., 2009;Kumari et al., 2011;Patlolla et al., 2012;Ghosh et al., 2012). However, these methods are very restricted for identifying the genotoxic effects of nanoparticles at the DNA level. DNA-based techniques are sensitive and selective assays that help to determine the genotoxic effects of environmental pollutants on DNA. One of these methods used for these aims is the intersimple sequence repeat (ISSR)-PCR assay. ISSR-PCR uses as primer microsatellite repeats (Zietkiewicz et al., 1994). The ISSR-PCR method is more sensitive than the random amplified polymorphic DNA assay (RAPD) (Correia et al., 2014;Bajpai et al., 2015), because of the exhibiting specificity of the sequence-tagged-site markers and high ratio of reproducibility potential owing to the use of longer primers (16-25 bp).

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“…Some toxicological studies have reported that the nanomaterials can be cytotoxic [387][388][389][390][391][392], genotoxic [391,[393][394][395][396][397][398][399], bactericidal [400][401][402][403][404], neurotoxic [405][406][407][408][409] and ecotoxic [410][411][412][413][414].…”

Section: Nanoecotoxicology Health Risks and Regulationsmentioning

confidence: 99%

Application Of Nanotechnology In Agriculture And Food Industry, Its Prospects And Risks

Jampílek

Kráľová

2015

Ecological Chemistry and Engineering S

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Nanoagrochemicals, such as nanopesticides, nanofertilizers or plant growth stimulating nanosystems, were primarily designed to increase solubility, enhance bioavailability, targeted delivery, controlled release and/or protection against degradation resulting in the reduced amount of applied active ingredients and finally in a decrease of dose-dependent toxicity/burden. This paper is a comprehensive up-to-date review related to the preparation and the biological activity of nanoformulations enabling gradual release of active ingredient into weeds and the body of pests and controlled release of nutrients to plants. The attention is also devoted to the decrease of direct environmental burden and economic benefits due to application of nanoformulations, where less amount of active ingredient is needed to achieve the same biological effect in comparison with bulk. The application of nanotechnology in the areas such as food packaging, food security, encapsulation of nutrients and development of new functional products is analysed. The use of nanoparticles in biosensors for detection of pathogens and contaminants as well as in DNA and gene delivery is discussed as well. Benefits and health risks of nanoagrochemicals are highlighted, and special attention is given to nanoecotoxicology and guidelines and regulatory documents related to the use of nanoformulations in agriculture and food industry.

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Cytogenetic and genotoxic effects of zinc oxide nanoparticles on root cells of Allium cepa

Cited by 353 publications

References 39 publications

Interaction of cobalt nanoparticles with plants: a cytogenetical aspect

Interaction of cobalt nanoparticles with plants: a cytogenetical aspect

Silver nanoparticles induced genotoxicity and oxidative stress in tomato plants

Application Of Nanotechnology In Agriculture And Food Industry, Its Prospects And Risks

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