Cytogenetic and Interphase FISH Analyses of 73 Basal Cell and Three Squamous Cell Carcinomas

Casalone, Rosario; Mazzola, D.; Righi, R.; Granata, Paola; Minelli, E.; Salvadore, Maurizio; Lombardo, M.; Bertani, E.

doi:10.1016/s0165-4608(99)00195-8

Cited by 18 publications

(24 citation statements)

References 15 publications

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“…Unfortunately, because of technical problems associated with the keratin and fat content of skin, interphase FISH studies of NMSCs have been extremely limited. As previously mentioned, trisomy 6 was confirmed by interphase FISH in direct BCC cultures (Casalone et al, 2000). Furthermore, Nangia et al (2001) demonstrated a correlation between trisomy 6 and metastatic potential in BCCs.…”

Section: Interphase Fishmentioning

confidence: 69%

“…Chromosomal aberrations are commonly observed in both benign and precursor (36% of KAs, 90% of SCCs in situ, 90% of SKs) and neoplastic (62% of BCCs and 95% of SCCs) skin lesions Rehman et al, 1996;Waring et al, 1996;Jin et al, 1998Jin et al, , 1999Jin et al, , 2001Casalone et al, 2000;Ashton et al, 2001Ashton et al, , 2003Clausen et al, 2002). It is well recognized that UVR induces DNA point mutations and small deletions (Sarasin, 1999;Matsumura and Ananthaswamy, 2004), but its involvement in gross chromosomal changes is unknown.…”

Section: Solar Uvr and Chromosomal Aberrationsmentioning

confidence: 99%

“…Casalone and colleagues (2000) attempted to overcome issues associated with cultured tumors by studying direct preparations (24 h) in addition to short-term (10-28 days) BCC tissue cultures. They demonstrated inconsistencies in chromosomal aberrations between direct preparations and short-term cultures, characterized by epithelial and fibroblastic growths, respectively (Casalone et al, 2000). This suggested that fibroblastic stroma as well as epithelial tissue is implicated in the neoplastic process.…”

Section: Karyotype Analysismentioning

confidence: 99%

“…In addition to their work on BCCs, Casalone and colleagues also investigated 3 SCC cases and showed numerous aberrations in direct preparations that were absent in short-term cultures (Casalone et al, 2000). These included þ6, þ8, þ9, þ11, þ16, þ21, À1, and À14.…”

Section: Karyotype Analysismentioning

confidence: 99%

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Cytogenetic alterations in nonmelanoma skin cancer: A review

Ashton

Carless

Griffiths

2005

Genes Chromosomes & Cancer

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Since the advent of cytogenetic analysis, knowledge about fundamental aspects of cancer biology has increased, allowing the processes of cancer development and progression to be more fully understood and appreciated. Classical cytogenetic analysis of solid tumors had been considered difficult, but new advances in culturing techniques and the addition of new cytogenetic technologies have enabled a more comprehensive analysis of chromosomal aberrations associated with solid tumors. Our purpose in this review is to discuss the cytogenetic findings on a number of nonmelanoma skin cancers, including squamous- and basal cell carcinomas, keratoacanthoma, squamous cell carcinoma in situ (Bowen's disease), and solar keratosis. Through classical cytogenetic techniques, as well as fluorescence-based techniques such as fluorescence in situ hybridization and comparative genomic hybridization, numerous chromosomal alterations have been identified. These aberrations may aid in further defining the stages and classifications of nonmelanoma skin cancer and also may implicate chromosomal regions involved in progression and metastatic potential. This information, along with the development of newer technologies (including laser capture microdissection and comparative genomic hybridization arrays) that allow for more refined analysis, will continue to increase our knowledge about the role of chromosomal events at all stages of cancer development and progression and, more specifically, about how they are associated with nonmelanoma skin cancer.

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Section: Interphase Fishmentioning

confidence: 69%

Section: Solar Uvr and Chromosomal Aberrationsmentioning

confidence: 99%

Section: Karyotype Analysismentioning

confidence: 99%

Section: Karyotype Analysismentioning

confidence: 99%

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Cytogenetic alterations in nonmelanoma skin cancer: A review

Ashton

Carless

Griffiths

2005

Genes Chromosomes & Cancer

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“…Conventional cytogenetics (CC) methods have shown that complex karyotypes are present in cSCC (Heim et al, 1988(Heim et al, , 1989Atkin and Fox, 1992;Mertens et al, 1992;Worsham et al, 1993;Aledo et al, 1998;Jin et al, 1999Jin et al, , 2002Casalone et al, 2000). Additional comparative genomic hybridization technology (CGH) studies showed that the most frequent chromosomal abnormalities in cSCC were gains of chromosome arms 3q, 17q, 4p, 14q, Xq, 5p, 9q, 8q, 17p, and 20q and losses of 9p, 3p, 13q, 17p, 11p, 8q, and 18p (Popp et al, 2000;Ashton et al, 2003;Clausen et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

CKS1B amplification is a frequent event in cutaneous squamous cell carcinoma with aggressive clinical behaviour

Salgado

Toll

Alameda

et al. 2010

Genes Chromosomes & Cancer

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Genetic mechanisms giving rise to the development of cutaneous squamous cell carcinoma (cSCC) are poorly understood and development of genomic high resolution techniques has led to a better knowledge of the genetic basis of several human cancers. In this study, 16 cSCC were analyzed using array comparative genomic hybridization (arrayCGH). The most common aberrations found were gains of 3q11q13, 1q21.3q25, 13q34, and 19p13, and losses of 1p36p31, 3p24p21, 10p15q22, and 13q11q21. We detected gains (3/16) and amplification (1/16) of the 1q21.1q21.3 region. A potential candidate gene in this region, CKS1B (1q21.2), was selected for validation in an independent cohort and correlations with clinicopathological features were carried out. CKS1B gene and protein status were analyzed using fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) in a series of 53 cSCC, 22 actinic keratoses (AK), and 10 normal skin samples. cSCC presented a higher frequency of chromosome 1 polysomy than AK (70% vs. 46%, P = 0.047). Association between CKS1B protein overexpression and both polysomy and amplification was demonstrated in cSCC (P < 0.001). Regarding amplifications, 11 cSCC patients (21%) presented CKS1B gene amplification. Interestingly, 8/11 (73%) patients who showed a CKS1B amplification had presented metastatic spread (mcSCC). Differences between the presence of CKS1B amplification and the presence or absence of mcSCC were observed (mcSCC [8/14] vs. cSCC [3/39]) (P < 0.001). Several drugs targeting CKS1B have been reported and may be useful for treating patients with cSCC and CKS1B amplifications.

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Tumors of the Skin

Mertens¹,

Heim²

2010

Cancer Cytogenetics

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Cytogenetic and Interphase FISH Analyses of 73 Basal Cell and Three Squamous Cell Carcinomas

Cited by 18 publications

References 15 publications

Cytogenetic alterations in nonmelanoma skin cancer: A review

Cytogenetic alterations in nonmelanoma skin cancer: A review

CKS1B amplification is a frequent event in cutaneous squamous cell carcinoma with aggressive clinical behaviour

Tumors of the Skin

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