Cytogenetic and molecular biologic alterations in human breast cancer: A review

Trent, Jeffrey M.

doi:10.1007/bf01806017

Cited by 68 publications

(43 citation statements)

References 26 publications

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“…In his review of human breast cytogenetics, based principally on unbanded cases, Sandberg (1980) stated that diploid metaphases are occasionally observed. Trent (1985) also cited the work by Wolman (1983), who found karyotypically normal diploid cells in primary mammary adenocarcinomas but not in metastatic effusions. These authors all concluded that acquisition of an abnormal karyotype is indicative of malignant progression rather than initial events in tumorigenesis.…”

Section: Resultsmentioning

confidence: 98%

“…These authors all concluded that acquisition of an abnormal karyotype is indicative of malignant progression rather than initial events in tumorigenesis. As mentioned by Trent (1985) and Smith et al (1985), it could be questioned whether diploid cells are really representative of the tumoral population. In culture since October 1985, cell line CAL51 has conserved a diploid karyotype with normal chromosomes.…”

Section: Resultsmentioning

confidence: 99%

“…Nevertheless, owing to the heterogenity and the diversity of mammary cancers, a great number of cell models is necessary to understand the reasons for this diversity and the effect of anticancer drugs on tumour cells.Cytogenetic studies of mammary adenocarcinoma cell lines are essential for comprehension of the pathogenesis of these cancers (Trent, 1985;Gebhart et al, 1986). The implication of chromosomal alterations in these pathologies has opened a new and promising route towards better knowledge of these cancers (Cervenka & Koulischer, 1973).…”

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confidence: 99%

“…Cytogenetic studies of mammary adenocarcinoma cell lines are essential for comprehension of the pathogenesis of these cancers (Trent, 1985;Gebhart et al, 1986). The implication of chromosomal alterations in these pathologies has opened a new and promising route towards better knowledge of these cancers (Cervenka & Koulischer, 1973).…”

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confidence: 99%

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Establishment and characterisation of a new tumorigenic cell line with a normal karyotype derived from a human breast adenocarcinoma

Gioanni

François²,

Zanghellini³

et al. 1990

Br J Cancer

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In vitro models are required for the study of these cancers, and several cell lines have already been established and characterised (Lafargues & Ozzello, 1958;Soule et al., 1973;Cailleau et al., 1974;Engel et al., 1978;Whitehead et al., 1983;Yamane et al., 1984;Chu et al., 1985;Vandewalle et al., 1987). Nevertheless, owing to the heterogenity and the diversity of mammary cancers, a great number of cell models is necessary to understand the reasons for this diversity and the effect of anticancer drugs on tumour cells.Cytogenetic studies of mammary adenocarcinoma cell lines are essential for comprehension of the pathogenesis of these cancers (Trent, 1985;Gebhart et al., 1986). The implication of chromosomal alterations in these pathologies has opened a new and promising route towards better knowledge of these cancers (Cervenka & Koulischer, 1973). Chromosomal alterations are generally numerous, and markers often demonstrate hyperploidy in these cancers (Sandberg, 1980). Demonstration of the minimum genetic alterations indispensable for cell transformation is difficult, and might be easier on cells with a karyotype closer to normal. Sandberg and Wolman mentioned the existence of such cells, but most of their results concerned karyotype studies without chromosome banding (Sandberg, 1980;Wolman, 1983 [-', hyaluronidase 25 IU for 20 ml, Hepes buffer 4.8 g 1' in distilled water). Cells were then fixed in acetic acid:methanol (1:3) and dropped onto grease-free, cooled slides for chromosome counting and examination. R bands were obtained by heat denaturation of the chromosomes according to the method of Dutrillaux and Lejeune (1971). Xenografted CAL51 cells were plated and studied in vitro in the same manner.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Establishment and characterisation of a new tumorigenic cell line with a normal karyotype derived from a human breast adenocarcinoma

Gioanni

François²,

Zanghellini³

et al. 1990

Br J Cancer

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“…In primary cell cultures of breast tumors, only a minority of cells is mitotic and few of these mitoses could be analyzed (22,23). This problem was further confounded by the observation that the few highly selected cells that could be analyzed karyologically differ greatly among even those from the same tumor (22,23).…”

Section: Discussionmentioning

confidence: 99%

Loss of heterozygosity in human ductal breast tumors indicates a recessive mutation on chromosome 13.

Lundberg¹,

Skoog²,

Cavenee³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

147

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The genotypes at chromosomal loci defined by recombinant DNA probes revealing restriction fragment length polymorphisms were determined in constitutional and tumor tissue from 10 cases of ductal breast cancer: eight premenopausal females and two males. Somatic loss of constitutional heterozygosity was observed at loci on chromosome 13 in primary tumor tissue from three females and one male. In two cases, specific loss ofheterozygosity at three distinct genetic loci along the length of the chromosome was observed. In another case, concurrent loss of alleles at loci on chromosomes 2, 13, 14, and 20 was detected, whereas a fourth case showed loss of heterozygosity for chromosomes 5 and 13. In each instance, the data were consistent with loss of one of the homologous chromosomes by mitotic nondisjunction. Analysis of loci on several other chromosomes showed retention of constitutional heterozygosity suggesting the relative specificity of the events. In contrast, similar analyses of other breast cancers, including comedocarcinoma, medullary carcinoma, and juvenile secretory carcinoma, showed no loss of alleles at loci on chromosome 13. These data indicate that the pathogenesis of ductal breast cancer may, in a substantial proportion of cases, involve unmasking of a recessive locus on chromosome 13 and suggest the involvement of such a locus in heritable forms of this disease.Breast cancer is the most frequent malignant tumor in females. Although most cases are sporadic, a familial aggregation is sometimes found; about 5% of all cases are estimated to be hereditary with an autosomal dominant mode of inheritance. These latter cases demonstrate a significantly earlier age of onset, an excess of multiple primary tumors, a longer life expectancy, and a greater proportion of affected males than the sporadic cases (1). A familial association between breast cancer and other histologically distinct tumors has been noted analogous to observations with embry-

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Comparative genomic hybridization as a tool in tumour cytogenetics

James

1999

J. Pathol.

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The quality of cytogenetic analysis of solid tumours has greatly improved in the past decade, but a number of technical difficulties remain which limit the characterization of solid tumour chromosomes by conventional cytogenetics alone. The identification of regions of chromosomal abnormality has been aided by the introduction of molecular cytogenetic techniques such as fluorescence in situ hybridization (FISH). Of these, a recently developed approach, comparative genomic hybridization (CGH), has had a particular impact on the cytogenetic analysis of solid tumours. It incorporates the sensitivity of in situ techniques and overcomes many of the drawbacks of conventional cytogenetic analysis. This review first outlines the CGH method, giving details for the preparation of DNA probes and target human metaphase chromosomes together with information on the in situ technique and data handling criteria used in our laboratory. It then presents an overview of some of the current applications of CGH, together with a discussion of future directions in the field. Copyright © 1999 John Wiley & Sons, Ltd.

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Cytogenetic and molecular biologic alterations in human breast cancer: A review

Cited by 68 publications

References 26 publications

Establishment and characterisation of a new tumorigenic cell line with a normal karyotype derived from a human breast adenocarcinoma

Establishment and characterisation of a new tumorigenic cell line with a normal karyotype derived from a human breast adenocarcinoma

Loss of heterozygosity in human ductal breast tumors indicates a recessive mutation on chromosome 13.

Comparative genomic hybridization as a tool in tumour cytogenetics

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