Cytogenetic and molecular markers reveal the complexity of the genus Piabina Reinhardt, 1867 (Characiformes: Characidae)

Pazian, Marlon F.; Pereira, Luiz Henrique Garcia; Shimabukuru-Dias, Cristiane Kioko; Oliveira, Cláudio; Foresti, Fausto

doi:10.1590/s1679-62252012005000015

Cited by 12 publications

(15 citation statements)

References 58 publications

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“…Indeed, even though some genera of Characidae share single NORs, a basal condition in teleosteans (Gornung, 2013), as reported in Tetragonopterus Cuvier (Alberdi & Fenocchio, 1997), Moenkhausia Eigenmann (Castro & Júlio Júnior, 2002), Bryconamericus Eigenmann (Marques et al, 2003) and Piabina Reinhardt (Pazian et al, 2012), most species in this family present multiple NORs (Kavalco & Moreira Filho, 2003).…”

Section: Discussionmentioning

confidence: 78%

Allopatric chromosomal variation in Nematocharax venustus Weitzman, Menezes & Britski, 1986 (Actinopterygii: Characiformes) based on mapping of repetitive sequences

et al. 2016

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Characiformes is the most cytogenetically studied group of freshwater Actinopterygii, but karyotypical data of several taxa remain unknown. This is the case of Nematocharax, regarded as a monotypic genus and characterized by marked sexual dimorphism. Therefore, we provide the first cytogenetic report of allopatric populations of Nematocharax venustus based on distinct methods of chromosomal banding and fluorescence in situ hybridization (FISH) with repetitive DNA probes (18S and 5S rDNA). The karyotype macrostructure was conserved in all specimens and populations, independently on sex, since they shared a diploid number (2n) of 50 chromosomes divided into 8m+26sm+14st+2a. The heterochromatin was mainly distributed at pericentromeric regions and base-specific fluorochrome staining revealed a single pair bearing GC-rich sites, coincident with nucleolar organizer regions (NORs). On the other hand, interpopulation variation in both number and position of repetitive sequences was observed, particularly in relation to 5S rDNA. Apparently, the short life cycles and restricted dispersal of small characins, such as N. venustus, might have favored the divergence of repetitive DNA among populations, indicating that this species might encompass populations with distinct evolutionary histories, which has important implications for conservation measures.Characiformes é o grupo de Actinopterygii de água doce mais estudado citogeneticamente, porém dados cariotípicos de vários taxa permanecem desconhecidos. Este é o caso de Nematocharax, considerado um gênero monotípico e caracterizado pelo acentuado dimorfismo sexual. Em vista disso, nós fornecemos a primeira descrição citogenética de populações alopátricas de Nematocharax venustus, baseada em métodos distintos de bandamento cromossômico e hibridação fluorescente in situ (FISH) com sondas de DNA repetitivo (DNAr 18S e 5S). A macroestrutura cariotípica mostrou-se conservada em todos os espécimes e populações, independentemente do sexo, uma vez que compartilharam um número diploide (2n) de 50 cromossomos dividido em 8m+26sm+14st+2a. A heterocromatina distribuiu-se principalmente nas regiões pericentroméricas e a coloração com fluorocromos base-específicos revelou um único par portador de sítios GC-ricos, coincidentes com as regiões organizadoras de nucléolo (RONs). Por outro lado, foi observada uma variação interpopulacional no número e na posição das sequências repetitivas, especialmente em relação ao DNAr 5S. Aparentemente, ciclos de vida curtos e dispersão restrita dos pequenos caracídeos, tal como N. venustus, podem ter favorecido a divergência do DNA repetitivo entre as populações, indicando que essa espécie pode englobar populações com distintas histórias evolutivas, o que tem implicações importantes para medidas de conservação.

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Section: Discussionmentioning

confidence: 78%

Allopatric chromosomal variation in Nematocharax venustus Weitzman, Menezes & Britski, 1986 (Actinopterygii: Characiformes) based on mapping of repetitive sequences

et al. 2016

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“…The authors suggested that the development of geographic barriers resulting from the formation of the Serra do Mar, resulted in a pattern of allopatric speciation [45]. Two surveys analyzing Piabina argentea from the Upper Paraná and São Francisco River basins with molecular and cytogenetic tools showed that the nominal species comprises a species complex with six distinct putative species [18,19]. Interestingly, this species shows a similar geographic distribution to K. moenkhausii and is a similar small Neotropical fish [46].…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, some recent studies have shown that many widely distributed species are actually cases of cryptic species complexes which had gone unrecognized [18][19][20][21][22][23].…”

Section: Extraction Pcr and Sequencingmentioning

confidence: 99%

Knodus moenkhausii (Characiformes: Characidae): one fish species, three hydrographic basins - a natural or anthropogenic phenomenon?

Souza¹,

Oliveira²,

Pereira³

2015

DNA Barcodes

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We used the DNA barcoding technique (COI and CytB markers) combined with GMYC analysis to characterize the genetics of the widely distributed Neotropical fish species Knodus moenkhausii from three different isolated hydrographic basins. Despite the fact that most of the Neotropical hydrographic basins have been isolated for millions of years, species could be shared between basins due to natural events (stream capture) or anthropogenic activities. Recent surveys, however, have shown that many widely distributed species are actually species complexes divided into previously unrecognized cryptic species. In this study, we tested the hypothesis that K. moenkhausii from three hydrographic basins represent a single panmictic species and discuss the most likely explanation of its present geographical distribution. The GMYC analysis revealed that all specimens of K. moenkhausii represent a single species: the intra- and intergroup minimum K2P genetic distances for both genes were zero and haplotypes were shared among the three hydrographic basins. This suggests there has been recent interchange of K. moenkhausii throughout the three hydrographic basins. It is likely that this is due to recent human activities, either the transposition of natural barriers or intentional introduction or accidental escape due to the ornamental fish trade.

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“…Cytogenetic information is not available for Spintherobolus and in species of Clade A (Guimarães et al 1995, Krinski et al 2008, Pazian et al 2012, Piscor et al 2013) and Clade B (Martins-Santos and Tavares 1986, Souza et al 1995, Alberdi and Fenocchio 1997, Mariguela et al 2011) karyotypes do not show the big metacentric pair observed in Rhoadsia altipinna . On the contrary, all the Characidae species belonging to Clade C are characterized by the presence of the first large metacentric chromosome pair as shown by many reports on Astyanax Baird & Girard, 1854 (Carvalho et al 2002), Oligosarcus Günther, 1864 (Shuhei et al 2007), Hollandichthys Eigenmann, 1910 (Carvalho et al 2002), Hemigrammus Gill, 1858 (Arefjev 1990), Moenkhausia Eigenmann, 1903 (Foresti et al 1989), Hyphessobrycon Durbin, 1908 (Arefjev 1990, Carvalho et al 2002, Mendes et al 2011), among others.…”

Section: Discussionmentioning

confidence: 99%

First description of the karyotype and localization of major and minor ribosomal genes in Rhoadsia altipinna Fowler, 1911 (Characiformes, Characidae) from Ecuador

Sánchez‐Romero¹,

Abad²,

Cordero³

et al. 2015

CCG

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Karyotypic features of Rhoadsia altipinna Fowler, 1911 from Ecuador were investigated by examining metaphase chromosomes through Giemsa staining, C-banding, Ag-NOR, and two-color-fluorescence in situ hybridization (FISH) for mapping of 18S and 5S ribosomal genes. The species exhibit a karyotype with 2n = 50, composed of 10 metacentric, 26 submetacentric and 14 subtelocentric elements, with a fundamental number FN=86 and is characterized by the presence of a larger metacentric pair (number 1), which is about 2/3 longer than the average length of the rest of the metacentric series. Sex chromosomes were not observed. Heterochromatin is identifiable on 44 chromosomes, distributed in paracentromeric position near the centromere. The first metacentric pair presents two well-defined heterochromatic blocks in paracentromeric position, near the centromere. Impregnation with silver nitrate showed a single pair of Ag-positive NORs localized at terminal regions of the short arms of the subtelocentric chromosome pair number 12. FISH assay confirmed these localization of NORs and revealed that minor rDNA clusters occur interstitially on the larger metacentric pair number 1. Comparison of results here reported with those available on other Characidae permit to hypothesize that the presence of a very large metacentric pair might represent a unique and derived condition that characterize one of four major lineages molecularly identified in this family.

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Cytogenetic and molecular markers reveal the complexity of the genus Piabina Reinhardt, 1867 (Characiformes: Characidae)

Cited by 12 publications

References 58 publications

Allopatric chromosomal variation in Nematocharax venustus Weitzman, Menezes & Britski, 1986 (Actinopterygii: Characiformes) based on mapping of repetitive sequences

Allopatric chromosomal variation in Nematocharax venustus Weitzman, Menezes & Britski, 1986 (Actinopterygii: Characiformes) based on mapping of repetitive sequences

Knodus moenkhausii (Characiformes: Characidae): one fish species, three hydrographic basins - a natural or anthropogenic phenomenon?

First description of the karyotype and localization of major and minor ribosomal genes in Rhoadsia altipinna Fowler, 1911 (Characiformes, Characidae) from Ecuador

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