Cytogenetic and toxicological effects of Punica granatum Linnaeus fruit peel hydroethanolic extract in mice

Vale, Everton Pantoja; Rego, Lucas Rodrigues do; Pureza, Danilo Dheyvison Nascimento; Silva, Paulo Goberlânio de Barros; Sousa, Francisco Fábio Oliveira de; Neto, Moacir de Azevedo Bentes Monteiro

doi:10.1016/j.sajb.2020.01.041

Cited by 15 publications

(11 citation statements)

References 37 publications

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“…Different doses of anacardic acid were evaluated up to 300 mg/kg, and no difference in the micronucleus frequency was observed in comparison to their controls [ 30 , 35 ]. A micronuclei frequency of 0.26 % was found in rats treated with 2000 mg/kg of hydroethanolic extract of leaves Anacardium occidentale , while the negative control had 0.11 % and over 1 % was found in the positive control group (cyclophosphamide – CYP 50 mg/kg) [ 36 ], these data are in agreement with those obtained in this study ( Table 3 ).…”

Section: Resultsmentioning

confidence: 99%

“…A blood drop was collected from each animal’s tail tip and dragged over a clean microscopic blade to obtain the blood smear, dried at room temperature, followed by fixation in absolute methanol for 5 min and a second drying at room temperature. The blades were stained with diluted Giemsa dye [ 34 ] for 20 min, washed to remove excess of dye, dried at room temperature and finally analyzed in an optical microscope (Opton TIM-2008) on the immersion objective (100x) [ 35 ].…”

Section: Materials E Methodsmentioning

confidence: 99%

“…Micronucleated polychromatic erythrocytes (PCEMNs) in peripheral blood samples were calculated from 2000 cells [ 35 ] and the micronucleus prevalence (in %) determined.…”

Section: Materials E Methodsmentioning

confidence: 99%

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Toxicological and genotoxic evaluation of anacardic acid loaded-zein nanoparticles in mice

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Section: Resultsmentioning

confidence: 99%

Section: Materials E Methodsmentioning

confidence: 99%

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Toxicological and genotoxic evaluation of anacardic acid loaded-zein nanoparticles in mice

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“…According to Vidal et al, the histopathological studies of mice treated with P. granatum hydroethanolic extract presented slight congestion of the liver (14). Vale et al showed that the treatment with P. granatum fruit peel didn't induce any serious lesions in the organs compared to the control (37). Chidambara et al reported that the administration of the peel extract followed by exposure to carbon tetrachloride showed no effect on the liver and suggested that the peel extract possesses a protective effect (18).…”

Section: Discussionmentioning

confidence: 99%

In vivo and in vitro evaluation of antioxidant, antihemolytic, acute toxicity effects and high-performance liquid chromatography analysis of an aqueous extract of Moroccan Punica granatum L.

Elkoraichi

Latif

Faqer

et al. 2022

J Herbmed Pharmacol

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Introduction: The use of pomegranates in Moroccan pharmacopeia is due to their healing and nutritional properties because of their richness in secondary molecules. The following study analyses the composition of the aqueous extract of Punica granatum peel and evaluates in vivo and in vitro antioxidant effects, hemolytic protection, and acute toxicity. Methods: Quantification of the plant extract was realized by high-performance liquid chromatography (HPLC). The hemolytic assay was used for erythrocyte protection, while the in vitro antioxidant effect was evaluated by 2, 20-azinobis-(3-ethylbenzothiazoneline-6-sulphonic acid) (ABTS) and reducing ferric power (RFP) assays. The in-vivo antioxidant activity was tested by measuring levels of lipid peroxidation (LPO) in serum. The toxicological study was tested by oral administration of the extract to four groups of mice for 21 days, followed by a histopathological examination of the spleen. Results: HPLC analysis showed the presence of some phenolic compounds such as coumarin, caffeic, gallic and syringic acids. The IC50 of the antioxidant assays were 254.49 ± 62.17 μg/mL and 40.265 ± 2.9 μg/mL for ABTS and reducing power, respectively. Furthermore, the thiobarbituric acid reactive substances (TBARS) assay showed the lowest levels at 150 mg/mL concentration. All of the concentrations used for hemolytic protection did not exceed 15% of hemolysis. Moreover, the toxicity test showed no sign of mortality, signs of weakness, or weight loss; also the histopathological examination of the spleen tissues showed the absence of any damage. Conclusion: The peel extract of P. granatum showed good potential and could be exploited as a natural antioxidant and antihemolytic remedy, leading to the development of new drugs.

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“…The concentration of DXR (15 mg/kg body weight -b.w.) was set according to previous studies (Franke et al, 2005;Venkatesh et al, 2007;Vale et al, 2020).…”

Section: Positive Control (Dna Damage Induction)mentioning

confidence: 99%

Cytogenetic evaluation of α, β-amirina obtained in resin of Protium heptaphylum (Aubl.) Marchand in polychromatics erythrocytes of mice swiss.

Rego¹,

Vale²,

Pureza³

et al. 2021

Int J Innov Educ Res

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The Amazon Rainforest has a great variety of medicinal plants, among them we can highlight the “Almecegueira” or “Breu Branco” (Protium heptaphylum) in Portuguese, the producer of a greenish-white resin that hardens when it touches the air, known by its gastroprotective and anti-inflammatory effects. These effects are attributed to a triterpene mixture of α and β amirine, predominant in the resin. The purpose of the study is to obtain a cytogenetic profile to the α, β-amirine mixture obtained in the resin of P. heptaphylum. For this, the micronucleus test was used in peripheral blood and bone marrow; administering solution in Swiss mice with the dosages of 1mg/Kg, 3mg/Kg, and 10mg/Kg, diluted in 5% DMSO, the effects were observed in 24h and 48h after the treatment. For the test in peripheral blood the mice’s caudal vein was punctured, while for the bone marrow test, the femurs of the animals were obtained from which bone marrow samples were taken. It was found that in peripheral blood, the administration of the compounds did not cause genotoxicity in 24h and 48h, in contrast, antigenotoxicity was, for concentrations 1; 3 and 10mg/kg, respectively 10%; 12%; 67% in 24h and 9%; 15%; 73% in 48h. In the bone marrow, no genotoxicity was observed, as for antigenotoxicity was observed that for concentrations 1; 3 e 10mg/kg the percentage of reduction was respectively: 11%, 15%, and 30% in 24h and 13% 16% 33% in 48h. It is concluded that the studied compound can be an alternative for treatments in the future since it presents low toxicity and high antigenotoxic potential.

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Cytogenetic and toxicological effects of Punica granatum Linnaeus fruit peel hydroethanolic extract in mice

Cited by 15 publications

References 37 publications

Toxicological and genotoxic evaluation of anacardic acid loaded-zein nanoparticles in mice

Toxicological and genotoxic evaluation of anacardic acid loaded-zein nanoparticles in mice

In vivo and in vitro evaluation of antioxidant, antihemolytic, acute toxicity effects and high-performance liquid chromatography analysis of an aqueous extract of Moroccan Punica granatum L.

Cytogenetic evaluation of α, β-amirina obtained in resin of Protium heptaphylum (Aubl.) Marchand in polychromatics erythrocytes of mice swiss.

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