Cytogenetic characterization, nuclear genome size, and pollen morphology of some Hedysarum L. taxa (Fabaceae) from Algeria, with emphasis on the origin of H. perrauderianum Coss. &amp; Durieu

Benhizia, Hayet; Benhizia, Yacine; Djeghar, Radhia; Pustahija, Fatima; Siljak‐Yakovlev, Sonja; Khalfallah, Nadra

doi:10.1007/s10722-020-01016-0

Cited by 6 publications

(6 citation statements)

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“…Therefore, variations in genome sizes, observed within legume family, can be explained by variability in the content of LTR retrotransposons (Macas et al, 2015;Ellis and Vershinin, 2020). In diploid Hedysarum species with 2n = 2x = 16, the amount of nuclear DNA ranged within 2C = 1.26-3.4 pg (Benhizia et al, 2021), which was relatively small for plants (Vitte and Bennetzen, 2006;Bennetzen and Wang, 2014;Wang et al, 2021). This fact was quite consistent with the ratio of low-copy and repetitive DNA sequences detected in genomes of the studied species of the sect.…”

Section: Discussionmentioning

confidence: 99%

“…Genomic DNAs of H. grandiflorum, H. zundukii, and H. dahuricum were isolated from young leaves using the CTAB method with minor modifications (Rogers and Bendich, 1985). Genome DNA low-coverage sequencing was performed at the Beijing Genomics Institute (BGISeq platform) (Shenzhen, Guangdong, China) according to the NGS protocol for generating 5-10 million of paired-end reads of 150 bp in length, which was at least 0.5-0.9x of the coverage of the Hedysarum genome (1C = 1,643 Mbp, Benhizia et al, 2021).…”

Section: Construction Of Schematic Map Of Species Distribution Areasmentioning

confidence: 99%

“…1 The comparative integrated bioinformatic analysis of repeatomes of H. grandiflorum, H. zundukii, and H. dahuricum was performed using RepeatExplorer/TAREAN pipelines (Novak et al, 2013(Novak et al, , 2017. For each studied species, the genomic reads were filtered by quality, and then 1,000,000 high-quality reads were randomly selected for further analyses, which corresponds to 0.09x of coverage of the genome Hedysarum (1C = 1,643 Mbp, Benhizia et al, 2021) and is within the limits recommended by the developers of these programs (genome coverage of 0.01-0.50x is recommended) (Novak et al, 2017) 1) by Primer3-Plus software (Untergasser et al, 2007).…”

Section: Construction Of Schematic Map Of Species Distribution Areasmentioning

confidence: 99%

“…The analysis of chromosome C-banding patterns, performed in the karyotype of H. coronarium L. originating from North-East Algeria, revealed three types of bands (terminal, intercalary, and pericentromeric) (Issolah et al, 2006). In four Algerian species, Hedysarum carnosum Desf., H. spinosissimum L., H. pallidum Desf., and H. naudinianum Coss., 35S rDNA loci, localized in one chromosome pair, and different numbers and position of 5S rDNA clusters (in one or two pairs) were detected by fluorescent in situ hybridization (FISH) (Benhizia et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Integration of Genomic and Cytogenetic Data on Tandem DNAs for Analyzing the Genome Diversity Within the Genus Hedysarum L. (Fabaceae)

et al. 2022

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The section Multicaulia is the largest clade in the genus Hedysarum L. (Fabaceae). Representatives of the sect. Multicaulia are valuable plants used for medicinal and fodder purposes. The taxonomy and phylogeny of the sect. Multicaulia are still ambiguous. To clarify the species relationships within sect. Multicaulia, we, for the first time, explored repeatomes of H. grandiflorum Pall., H. zundukii Peschkova, and H. dahuricum Turcz. using next-generation sequencing technologies and a subsequent bioinformatic analysis by RepeatExplorer/TAREAN pipelines. The comparative repeatome analysis showed that mobile elements made up 20–24% (Class I) and about 2–2.5% (Class II) of their repetitive DNAs. The amount of ribosomal DNA varied from 1 to 2.6%, and the content of satellite DNA ranged from 2.7 to 5.1%. For each species, five high confident putative tandem DNA repeats and 5–10 low confident putative DNA repeats were identified. According to BLAST, these repeats demonstrated high sequence similarity within the studied species. FISH-based mapping of 35S rDNA, 5S rDNA, and satDNAs made it possible to detect new effective molecular chromosome markers for Hedysarum species and construct the species karyograms. Comparison of the patterns of satDNA localization on chromosomes of the studied species allowed us to assess genome diversity within the sect. Multicaulia. In all studied species, we revealed intra- and interspecific variabilities in patterns of the chromosomal distribution of molecular chromosome markers. In H. gmelinii Ledeb. and H. setigerum Turcz. ex Fisch. et Meyer, similar subgenomes were detected, which confirmed the polyploid status of their genomes. Our findings demonstrated a close genomic relationship among six studied species indicating their common origin and confirmed the taxonomic status of H. setigerum as a subspecies of H. gmelinii as well as the validity of combining the sect. Multicaulia and Subacaulia into one sect. Multicaulia.

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Section: Discussionmentioning

confidence: 99%

Section: Construction Of Schematic Map Of Species Distribution Areasmentioning

confidence: 99%

Section: Construction Of Schematic Map Of Species Distribution Areasmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Integration of Genomic and Cytogenetic Data on Tandem DNAs for Analyzing the Genome Diversity Within the Genus Hedysarum L. (Fabaceae)

et al. 2022

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“…Hedysarum have the basic chromosome number x = 7 unlike the species from other sections having x = 8 [ 4 , 21 , 25 ]. FISH-based chromosomal localization of rRNA genes was carried out in five Algerian species [ 26 , 27 ]. Molecular cytogenetic analysis of other Hedysarum species has not been conducted, and intra- or interspecific variability in chromosome localization of rRNA genes still remains uncertain.…”

Section: Introductionmentioning

confidence: 99%

Molecular Cytogenetics of Eurasian Species of the Genus Hedysarum L. (Fabaceae)

Yurkevich

Samatadze

Selyutina

et al. 2021

Plants

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The systematic knowledge on the genus Hedysarum L. (Fabaceae: Hedysareae) is still incomplete. The species from the section Hedysarum are valuable forage and medicinal resources. For eight Hedysarum species, we constructed the integrated schematic map of their distribution within Eurasia based on currently available scattered data. For the first time, we performed cytogenomic characterization of twenty accessions covering eight species for evaluating genomic diversity and relationships within the section Hedysarum. Based on the intra- and interspecific variability of chromosomes bearing 45S and 5S rDNA clusters, four main karyotype groups were detected in the studied accessions: (1) H.arcticum, H. austrosibiricum, H. flavescens, H. hedysaroides, and H. theinum (one chromosome pair with 45S rDNA and one pair bearing 5S rDNA); (2) H. alpinum and one accession of H. hedysaroides (one chromosome pair with 45S rDNA and two pairs bearing 5S rDNA); (3) H. caucasicum (one chromosome pair with 45S rDNA and one chromosome pair bearing 5S rDNA and 45S rDNA); (4) H. neglectum (two pairs with 45S rDNA and one pair bearing 5S rDNA). The species-specific chromosomal markers detected in karyotypes of H. alpinum, H. caucasicum, and H. neglectum can be useful in taxonomic studies of this section.

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Influence of ecological conditions of various habitats on individual morpho-biological and physiological features of Hedysarum grandiflorum pall seeds

Sajfutdinova,

Kosolapov,

Cherniavskih

et al. 2023

MATERIALS v INTERNATIONAL YOUTH APPLIED RESEARCH FORUM “OIL CAPITAL”: Conference Series “OIL CAPITAL”

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Cytogenetic characterization, nuclear genome size, and pollen morphology of some Hedysarum L. taxa (Fabaceae) from Algeria, with emphasis on the origin of H. perrauderianum Coss. & Durieu

Cited by 6 publications

References 43 publications

Integration of Genomic and Cytogenetic Data on Tandem DNAs for Analyzing the Genome Diversity Within the Genus Hedysarum L. (Fabaceae)

Integration of Genomic and Cytogenetic Data on Tandem DNAs for Analyzing the Genome Diversity Within the Genus Hedysarum L. (Fabaceae)

Molecular Cytogenetics of Eurasian Species of the Genus Hedysarum L. (Fabaceae)

Influence of ecological conditions of various habitats on individual morpho-biological and physiological features of Hedysarum grandiflorum pall seeds

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