Cytogenetic characterization of the sole Solea senegalensis (Teleostei: Pleuronectiformes: Soleidae): Ag-NOR, (GATA) n , (TTAGGG) n and ribosomal genes by one-color and two-color FISH

Cross, Ismael; Merlo, Alejandro Serani; Manchado, Manuel; Infante, Carlos; Cañavate, José Pedro; Rebordinos, Laureana

doi:10.1007/s10709-005-5928-9

Cited by 54 publications

(47 citation statements)

References 40 publications

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“…24 (Wang et al 2010). This phenomenon is not unusual; similar occurrences have been described in other organisms and attributed to structural changes such as duplications and/or deletions (Cross et al 2006, Fujiwara et al 2007. Therefore, both the diversified types of NOR locations and NOR size variations indicate that certain chromosome rearrangements must have occurred in the NOR-bearing chromosome pairs during karyotype evolution of the genus Epinephelus (Wang et al 2010).…”

Section: Resultssupporting

confidence: 75%

Cytogenetic Comparison and Chromosome Localization of the Nucleolar Organizer Region of Four Grouper Genera (Pisces, Epinephelinae) from Thailand

et al. 2013

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SummaryWe report the first cytogenetic comparison of four grouper genera from Thailand. Kidney cell samples were taken from the blueline hind (Cephalopholis formosa), humpback grouper (Cromileptes altivelis), orange-spotted grouper (Epinephelus coioides), and leopard coralgrouper (Plectropomus leopardus). The mitotic chromosome samples were prepared directly from the kidney cells. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that diploid chromosome numbers of Ce. formosa, Cr. altivelis, E. coioides, and P. leopardus were 2n=48 for all species, and the fundamental numbers (NF) were 52, 52, 50, and 48, respectively. The presence of metacentric, submetacentric, acrocentric, and telocentric chromosomes were 2-0-2-44, 0-2-2-44, 0-0-2-46, and 0-0-0-48, respectively. After the Ag-NOR banding technique, one pair of nucleolar organizer regions (NORs) was observed on the short arm telomeric region of

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Section: Resultssupporting

confidence: 75%

Cytogenetic Comparison and Chromosome Localization of the Nucleolar Organizer Region of Four Grouper Genera (Pisces, Epinephelinae) from Thailand

et al. 2013

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“…The number and location of NORs in chromosomes can be variable among populations and/or closely related species, thus representing a useful cytotaxonomic marker for phylogenetic reconstruction in some groups (Cross et al 2006). Structural rearrangements might be a cause of variation in either chromosomal location or frequency of these regions (Gu andHua 2003, Shan et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Mapping of Ribosomal DNA by FISH, EcoRI Digestion and Replication Bands in the Cardinalfish Apogon americanus (Perciformes)

2010

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Summary Reef fishes of the order Perciformes represent an interesting group for both genetic and cytogenetic studies as their evolutionary pattern, usually unclear, might range from dynamic to quite stable. A karyotype with 2nϭ48 acrocentric chromosomes is considered basal for this group, whereas karyotypes with other formulae are regarded as derived. Some Perciformes families present a high karyotypic diversification such as Apogonidae, although cytogenetic reports in this family along South Atlantic were absent so far. In the present work, cytogenetic analyses were carried out in Apogon americanus from Northeastern Brazil, revealing a modal diploid number of 2nϭ36 (12mϩ6smϩ16stϩ2a; FNϭ70) and a remarkable presence of large metacentric pairs. The AgNORs were located on short arms of the 8th pair, at telomeric position, coincident with hybridization signals obtained by FISH using 18S rDNA probes. The 5S minor ribosomal genes are distributed in 2 chromosomal pairs, not syntenic to the major ribosomal subunits. Heterochromatic blocks are located mainly at pericentromeric position in most of chromosomal pairs. The early replication pattern showed longitudinal bands with both early and late replication in the heterochromatin segments, being very similar to the pattern obtained after chromosomal digestion with EcoRI restriction enzyme. The present data, coupled with previous reports in other species, suggest a karyotypic evolution mainly driven by Robertsonian fusions (Rb) and pericentric inversions. In spite of the structural rearrangements, some features in the DNA composition and specific regions, such as 5S and 18S rDNA sites, reflect symplesiomorphic karyotype traits, shared by other clades in Perciformes.

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“…This may also explain why most vertebrates maintain this configuration. However, H. wyckii is the only species of the Bagridae family in which the 2 ribosomal genes are co-located, a scarce situation among fishes that has been reported in some species such as Prochilodus lineatus (Jesus and MoreiraFilho, 2003), Prochilodus argenteus (Hatanaka and Galetti, 2004), Salmo salar (Pendás et al, 1994), Oncorhynchus mykiss (Morán et al, 1996), fish of the sturgeon family (Fontana et al, 2003), and others (Cross et al, 2006;Marquioni et al, 2013).…”

Section: Physical Chromosome Mapping Of 5s and 18s Rdna Sequencesmentioning

confidence: 99%

Comparative cytogenetic mapping of rRNA genes among naked catfishes: implications for genomic evolution in the Bagridae family

et al. 2014

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ABSTRACT. In the present study, the karyotype and chromosomal characteristics of 9 species of the Bagridae fish family were investigated using conventional Giemsa staining as well as dual-color fluorescence in situ hybridization to detect the 18S and 5S rDNA sites. In addition to describing the karyotype of several Bagridae catfishes, we established molecular cytogenetic techniques to study this group. The 9 species contained a diploid chromosomal number, varying from 50 (Pseudomystus siamensis) to 62 (Hemibagrus wyckii), while none contained heteromorphic sex chromosomes. 18S rDNA sites were detected in only 1 chromosomal pair among all species evaluated. However, 3 different patterns were observed for the distribution of the 5S rDNA: 2 sites were found in the genus Mystus and in P. siamensis, multiple sites were observed in the genus Hemibagrus, and a syntenic condition for the 18S and 5S rDNA sites was identified in H. wyckii. The extensive variation in the number and chromosomal position of rDNA clusters observed among these Bagridae species may be related to the intense evolutionary dynamics of rDNA-repeated units, which generates divergent chromosomal distribution patterns even among closely related species. In summary, the distribution of repetitive DNA sequences provided novel, useful information regarding the evolutionary relationships between Bagridae fishes.

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Cytogenetic characterization of the sole Solea senegalensis (Teleostei: Pleuronectiformes: Soleidae): Ag-NOR, (GATA) n , (TTAGGG) n and ribosomal genes by one-color and two-color FISH

Cited by 54 publications

References 40 publications

Cytogenetic Comparison and Chromosome Localization of the Nucleolar Organizer Region of Four Grouper Genera (Pisces, Epinephelinae) from Thailand

Cytogenetic Comparison and Chromosome Localization of the Nucleolar Organizer Region of Four Grouper Genera (Pisces, Epinephelinae) from Thailand

Mapping of Ribosomal DNA by FISH, EcoRI Digestion and Replication Bands in the Cardinalfish Apogon americanus (Perciformes)

Comparative cytogenetic mapping of rRNA genes among naked catfishes: implications for genomic evolution in the Bagridae family

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