Cytogenetic, cross-mating and molecular evidence of four cytological races of Anopheles crawfordi (Diptera: Culicidae) in Thailand and Cambodia

Saeung, Atiporn; Baimai, Visut; Thongsahuan, Sorawat; Otsuka, Yasushi; Srisuka, Wichai; Taai, Kritsana; Somboon, Pradya; Suwonkerd, Wannapa; Sochanta, Tho; Choochote, Wej

doi:10.1016/j.crvi.2014.08.001

Cited by 4 publications

(4 citation statements)

References 36 publications

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“…The climate of these two provinces is quite different, i.e., Ubon Ratchathani Province has a tropical wet and dry climate, whereas Phang Nga Province is located on the shore to the Andaman Sea, and has heavy rain. Our results are in accordance with Saeung et al. (2014) .…”

Section: Discussionsupporting

confidence: 93%

Evidence to Support a Conspecific Nature of Allopatric Cytological Races of Anopheles nitidus (Diptera: Culicidae) in Thailand

Songsawatkiat

Baimai

Thongsahuan

et al. 2014

Journal of Insect Science

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Metaphase karyotype investigation on two allopatric strains of Anopheles nitidus Harrison, Scanlon, and Reid (Diptera: Culicidae) was conducted in Thailand during 2011–2012. Five karyotypic forms, i.e., Form A (X 1 , Y 1 ), Form B (X 1 , Y 2 ), Form C (X 2 , Y 3 ), Form D (X 1 , X 3 , Y 4 ), and Form E (X 1 , X 2 , X 3 , Y 5 ) were obtained from a total of 21 isofemale lines. Forms A, B, and C were confined to Phang Nga Province, southern Thailand, whereas Forms D and E were restricted to Ubon Ratchathani Province, northeastern Thailand. Cross-mating experiments among the five isofemale lines, which were representative of five karyotypic forms of An. nitidus , revealed genetic compatibility by providing viable progenies and synaptic salivary gland polytene chromosomes through F 2 generations. The results suggest that the forms are conspecific, and An . nitidus comprises five cytological races. The very low intraspecific sequence variations (average genetic distances = 0.002–0.008) of the nucleotide sequences in ribosomal DNA (internal transcribed spacer 2) and mitochondrial DNA (cytochrome c oxidase subunits I and II) among the five karyotypic forms were very good supportive evidence.

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Section: Discussionsupporting

confidence: 93%

Evidence to Support a Conspecific Nature of Allopatric Cytological Races of Anopheles nitidus (Diptera: Culicidae) in Thailand

Songsawatkiat

Baimai

Thongsahuan

et al. 2014

Journal of Insect Science

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“…identification is the main key for success in obtaining robust information. Consequently, ribosomal (ITS2) and mitochondrial (COI and COII) DNA have been used intensively and widely in the Oriental region for recognition of the species members of the Hyrcanus Group [1,[32][33][34][35][36][37][38][39][40][41][42]. Nonetheless, information on COI barcoding sequences for identification of eight species of the Hyrcanus Group in Thailand is lacking.…”

Section: Discussionmentioning

confidence: 99%

“…paraliae (accession number AB733031) [49], An. peditaeniatus (accession numbers AB539069 and AB715091) [42], and An. sinensis (accession number AY444351) [33].…”

Section: Discussionmentioning

confidence: 99%

DNA barcoding for the identification of eight species members of the Thai Hyrcanus Group and investigation of their stenogamous behavior

Wijit¹,

Saeung²,

Baimai³

et al. 2013

Comptes Rendus. Biologies

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Eight species members of the Thai Hyrcanus Group were identified based on the intact morphology and molecular analysis (COI barcoding, 658 bp) of F 1 -progenies. Five isofemale lines of each species were pooled in order to establish stock colonies. A stenogamous colony of each species was investigated by making 200 and 300 newly emerged adult females and males co-habit in a 30 cm cubic cage for one week. After ovipositon, the spermathecae of females were examined for sperms. The results revealed that Anopheles argyropus, Anopheles crawfordi, Anopheles nitidus, Anopheles pursati, Anopheles sinensis, Anopheles nigerrimus, Anopheles paraliae and Anopheles peditaeniatus yielded insemination rates of 0%, 0%, 0%, 31%, 33%, 42%, 50% and 77%, respectively. Continuous selection to establish stenogamous colonies indicated that An. sinensis, An. pursati, An. nigerrimus, An. paraliae and An. peditaeniatus provided insemination rates of 33-34%, 27-31%, 42-58%, 43-57% and 61-86% in 1, 2, 5, 6 and 20 generations of passages, respectively.

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“…The accuracy of morphological identification depends on the known ability to differentiate species and those held within species complexes cannot usually be differentiated morphologically. Cross-mating tests, mitotic and meiotic karyotypes, and molecular procedures are applied to identify sibling species within complexes [ 3 – 5 ]. Sibling species held within complexes often express different behaviours and bionomics, therefore, it is crucial to utilize molecular techniques to identify the mosquito specimens to species level, when examining the mosquito geography, ecology, and biology [ 3 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

A systematic review: is Anopheles vagus a species complex?

Dalilah,

Syafruddin,

Saleh

et al. 2024

Malar J

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Background Anopheles vagus (subgenus Cellia) has been identified as a vector for malaria, filariasis, and Japanese encephalitis in Asia. Sporozoites of Plasmodium falciparum and Plasmodium vivax have been found in this zoophilic mosquito in Asia and Indonesia. This study systematically reviews publications regarding An. vagus species, variation, bio-ecology, and malaria transmission in various localities in Asia, especially Indonesia, to determine whether the current data support An. vagus as a species complex. Methods The databases Pubmed, Scopus, Europe PMC, and Proquest were searched to identify information regarding the morphology, karyotypes, polytene chromosome, cross-mating, ecology, and molecular identification of An. vagus was then evaluated to determine whether there were possible species complexes. Results Of the 1326 articles identified, 15 studies were considered for synthesis. The Anopheles spp. samples for this study came from Asia. Eleven studies used morphology to identify An. vagus, with singular studies using each of karyotype identification, chromosomal polytene identification, and cross-breeding experiments. Ten studies used molecular techniques to identify Anopheles spp., including An. vagus. Most studies discovered morphological variations of An. vagus either in the same or different areas and ecological settings. In this review, the members of An. vagus sensu lato grouped based on morphology (An. vagus, An. vagus vagus, An. vagus limosus, and An. limosus), karyotyping (form A and B), and molecular (An. vagus genotype A and B, An. vagus AN4 and AN5). Genetic analysis revealed a high conservation of the ITS2 fragment among members except for the An. vagus genotype B, which was, in fact, Anopheles sundaicus. This review also identified that An. vagus limosus and An. vagus vagus were nearly identical to the ITS2 sequence. Conclusion Literature review studies revealed that An. vagus is conspecific despite the distinct morphological characteristic of An. vagus and An. limosus. Further information using another barcoding tool, such as mitochondrial COI and ND6 and experimental cross-mating between the An. vagus and An. limosus may provide additional evidence for the status of An. vagus as a species complex.

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Cytogenetic, cross-mating and molecular evidence of four cytological races of Anopheles crawfordi (Diptera: Culicidae) in Thailand and Cambodia

Cited by 4 publications

References 36 publications

Evidence to Support a Conspecific Nature of Allopatric Cytological Races of Anopheles nitidus (Diptera: Culicidae) in Thailand

Evidence to Support a Conspecific Nature of Allopatric Cytological Races of Anopheles nitidus (Diptera: Culicidae) in Thailand

DNA barcoding for the identification of eight species members of the Thai Hyrcanus Group and investigation of their stenogamous behavior

A systematic review: is Anopheles vagus a species complex?

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