Cytogenetic damage in peripheral blood cultures of Chaetophractus villosus exposed in vivo to a glyphosate formulation (Roundup)

Rossi, Luis Francisco; Luaces, Juan Pablo; Palermo, Ana María; Merani, María Susana; Mudry, Marta Dolores

doi:10.1016/j.ecoenv.2018.03.046

Cited by 11 publications

(2 citation statements)

References 61 publications

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“…The effect, however, has erroneously attributed to glyphosate (Cai et al, 2017). Among mammals, significant increases in chromosome aberration (including chromatid breaks) frequencies and sister chromatid exchanges per cell were seen in large hairy armadillo Chaetophractus villosus peripheral blood lymphocytes upon treatment with Roundup Full II R at doses corresponding to glyphosate concentrations of 0.065-0.26 mg/ml, confirming genotoxicity of the formulated glyphosate-based herbicide, evaluated by cellular and genetic biomarkers e.g., the mitotic index, cell proliferation kinetics, as well as frequencies of chromosome aberrations and sister chromatid exchanges (Luaces et al, 2017;Rossi et al, 2018).…”

Section: Registration Of Glyphosate In the European Unionmentioning

confidence: 81%

Re-registration Challenges of Glyphosate in the European Union

Székacs

Darvas

2018

Front. Environ. Sci.

106

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Section: Registration Of Glyphosate In the European Unionmentioning

confidence: 81%

Re-registration Challenges of Glyphosate in the European Union

Székacs

Darvas

2018

Front. Environ. Sci.

106

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“…Wherever organisms are exposed to commercial formulations, the active ingredients and the excipients separately may produce different results. Studies evaluating the toxicity and genotoxicity of these complex mixtures are therefore important (Rossi et al 2018). The bioindicator A. cepa was used to evaluate the genotoxicity of Roundup®, a non-selective herbicide widely used worldwide, and its active ingredient, glyphosate isopropylamine.…”

Section: A Cepa As Bioindicator Of Herbicidal Effectsmentioning

confidence: 99%

An evaluation for the standardization of theAllium cepatest as cytotoxicity and genotoxicity assay

Firbas²,

et al. 2018

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A general report on the use of the Allium test as cytotoxicological and genotoxicological assay is proposed, with particular emphasis about the standardization of the test in several common applications. The intraspecific variation in Allium cepa has been overlooked, as in most investigations no mention is made about origin and denomination of the onion cultivar used. A standardization of the used material would allow a better generalization of the results, since we cannot be sure that all cultivars would give the same response. A more frequent use of transmission electron microscopy (TEM) investigation is proposed. Even if relatively time consuming and not available in all laboratories, it may help to better understand the mechanism of cytotoxicity, since many morphological characters may appear similar but be arisen from different processes observable only with TEM. About statistical testing, tests other than chi-squared may be used in case of a lower amount of data. The most commonly used statistical tests are the parametric tests ANOVA and Student's t, and the nonparametric tests Kruskal-Wallis and Mann-Whitney U, for analysis of variance. Tests should be used also to assess the minimal sample dimension for obtaining significance, since data collection (microscope observation) appears to be one of the main bottle necks of the test. Also the use of the Allium test for testing liposomes and other nanovectors for drug delivery is proposed, in order to assess the cytotoxicity of these types of medium and the possible increase in cytotoxicity of the associated drug.

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