Cytogenetic effects of silver and gold nanoparticles on Allium cepa roots

Debnath, Pampa; Mondal, Naba Kumar; Hajra, Amita; Das, Chittaranjan

doi:10.1016/j.jgeb.2018.07.007

Cited by 38 publications

(24 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Figures 6 and 7 present the charts of logarithmic dependence A = a*ln(C) + b of the shoot growth activity on the concentration of Ag/Au nanoparticles and G. urbanum L extract respectively for lupine, corn, flax, Chia, tomato and garden cress. The obtained study results confirm that metal nanoparticles can exhibit both positive and negative effect on higher plants [27]. One example of that are the studies demonstrating the impact of silver nanoparticles on crops like barley [30], wheat [31], Brassica [32] and radish [33], in which no significant negative impact of nanoparticles was found on any plant species.…”

Section: Determination Of the Inhibitory Concentration 50% (Ic50)supporting

confidence: 73%

“…According to the literature, small nanoparticles (< 50 nm) are able to easily penetrate and pass into the lymphatic system. Subsequently, they can reach and harm the vital organs and body tissues [27]. Nanoparticles of various sizes are taken up by plants, translocated to shoots and accumulated in various aerial parts, which increases the possibility that they will be cycling in the ecosystem through various trophic levels.…”

Section: Determination Of the Inhibitory Concentration 50% (Ic50)mentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Phytotoxicity of Bimetallic Ag/Au Nanoparticles Synthesized Using Geum urbanum L.

Szymański

Dobrucka

2020

J Inorg Organomet Polym

View full text Add to dashboard Cite

The growing production and wider application of metal nanoparticles gives rise to many concerns about their release to natural ecosystems. It is very important to be aware of the harmful impact of nanoparticles on living organisms, including plants. Therefore, it is of vital significance to explore the impact of metal nanoparticles on plants. This work assessed the phytotoxicity of bimetallic Ag/Au nanoparticles and Geum urbanum L. extract. The obtained bimetallic Ag/Au nanoparticles were characterized by UV–vis spectrophotometry (UV–vis), Transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR). The microscopic studies enabled the determination of the size of the obtained nanoparticles, which was 50 nm. The wide range of concentrations evaluated in the course of the study made it possible to observe changes in selected plants (seeds of Lepidium sativum, Linum flavum, Zea mays, Solanum lycopersicum var. Cerasiforme and Salvia hispanica-Chia) caused by a stress factor. The studies showed that the solution of Ag/Au nanoparticles was most toxic to flax (IC50 = 9.83 × 10–6/9.25 × 10–6 mg/ml), and least toxic to lupine (IC50 = 1.23 × 10–3/1.16 × 10–3 mg/ml). Moreover, we studied the toxicity of Geum urbanum extract. The extracts diluted to 0.00875 mg/ml stimulated the growth of lupine, flax and garden cress; extracts diluted to 0.175 mg/ml stimulated the growth of Chia and tomatoes; and extracts diluted to 0.00875 mg/ml stimulated the growth of corn. G. urbanum extract was most toxic to lupine (IC50 = 0.374 mg/ml), and least toxic to corn (IC50 = 4.635 mg/ml).

show abstract

Section: Determination Of the Inhibitory Concentration 50% (Ic50)supporting

confidence: 73%

Section: Determination Of the Inhibitory Concentration 50% (Ic50)mentioning

confidence: 99%

Evaluation of Phytotoxicity of Bimetallic Ag/Au Nanoparticles Synthesized Using Geum urbanum L.

Szymański

Dobrucka

2020

J Inorg Organomet Polym

View full text Add to dashboard Cite

show abstract

“…Table 1 summarizes the cytotoxic and genotoxic response registered after the exposure of Allium cepa to different concentrations of diverse AgNPs formulations from published data. Most of the formulations assessed reported cytotoxic and genotoxic damage, mainly those that lack of coating agent and the biogenically produced nanoparticles [ 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

Argovit™ Silver Nanoparticles Effects on Allium cepa: Plant Growth Promotion without Cyto Genotoxic Damage

Casillas-Figueroa¹,

Arellano-García²,

Leyva³

et al. 2020

Nanomaterials

View full text Add to dashboard Cite

Due to their antibacterial and antiviral effects, silver nanoparticles (AgNP) are one of the most widely used nanomaterials worldwide in various industries, e.g., in textiles, cosmetics and biomedical-related products. Unfortunately, the lack of complete physicochemical characterization and the variety of models used to evaluate its cytotoxic/genotoxic effect make comparison and decision-making regarding their safe use difficult. In this work, we present a systematic study of the cytotoxic and genotoxic activity of the commercially available AgNPs formulation Argovit™ in Allium cepa. The evaluated concentration range, 5–100 µg/mL of metallic silver content (85–1666 µg/mL of complete formulation), is 10–17 times higher than the used for other previously reported polyvinylpyrrolidone (PVP)-AgNP formulations and showed no cytotoxic or genotoxic damage in Allium cepa. Conversely, low concentrations (5 and 10 µg/mL) promote growth without damage to roots or bulbs. Until this work, all the formulations of PVP-AgNP evaluated in Allium cepa regardless of their size, concentration, or the exposure time had shown phytotoxicity. The biological response observed in Allium cepa exposed to Argovit™ is caused by nanoparticles and not by silver ions. The metal/coating agent ratio plays a fundamental role in this response and must be considered within the key physicochemical parameters for the design and manufacture of safer nanomaterials.

show abstract

“…The results indicated that cobalt oxide NPs induced DNA strand breaks and apoptosis. Also, NPs cause chromosomal aberrations as several researchers have discussed in their study of these effects in higher plants (Kumari et al, 2009;Ghosh et al, 2010;Landa et al, 2012;Mukherjee et al, 2016;Debnath et al, 2018).…”

Section: Description Concentration % Reference Low Highmentioning

confidence: 99%

Cytogenetic and molecular assessment of some nanoparticles using Allium sativum assay

Al-Ahmadi¹

2019

Afr. J. Biotechnol.

View full text Add to dashboard Cite

One of the primary objectives in agriculture is providing high-quality crops to consumers. Multiple techniques and methods are utilized to achieve this objective, including nanotechnology that depends on the use of very small materials, which will help in decreasing the amounts usually used with similar effects. Nanomaterials are used as fertilizers and also as component of nano-pesticides for plants. Despite their benefits, however, studies have noted their potential for cytotoxicity and genotoxicity. In this study, five nanoparticles (NPs) were tested to assess their effects on plants. The chromosomal aberration assay was used. The results showed that some NPs decreased the mitotic index (MI) significantly, which indicates the NPs' potential cytotoxicity. In addition, different NPs' treatments caused different types of chromosomal abnormalities e.g., chromosomes stickiness and disturbance of the metaphase and anaphase, lagging chromosomes, bridges, disturbed poles, micronuclei, smetaphase, s-telophase, c-metaphase and bi-nucleus cells. All treatments had significant effects at p≤005. Treatments with NPs concentrations for 24 h affected the DNA content, AlO 2 and Fe 3 O 4 NPs' increased the DNA content, while CeO 2 , TiO 2 and Ag NPs' decreased it. High concentrations of the tested NPs decreased the DNA content. The study results showed that CeO 2 was the most harmful NP compared to the control and other NPs. Some types of chromosome abnormalities such as lagging chromosomes, bridge, and micronuclei indicate potential genotoxicity for these NPs. Despite of the positive effects, they also had negative side effects such as decreasing the MI and increasing the occurrence of different types of chromosomal abnormalities.

show abstract

Cytogenetic effects of silver and gold nanoparticles on Allium cepa roots

Cited by 38 publications

References 34 publications

Evaluation of Phytotoxicity of Bimetallic Ag/Au Nanoparticles Synthesized Using Geum urbanum L.

Evaluation of Phytotoxicity of Bimetallic Ag/Au Nanoparticles Synthesized Using Geum urbanum L.

Argovit™ Silver Nanoparticles Effects on Allium cepa: Plant Growth Promotion without Cyto Genotoxic Damage

Cytogenetic and molecular assessment of some nanoparticles using Allium sativum assay

Contact Info

Product

Resources

About