Cytogenetic instability in populations with residential proximity to open-pit coal mine in Northern Colombia in relation to PM10 and PM2.5 levels

Espitia-Pérez, Lyda; Silva, Juliana da; Espitia-Pérez, Pedro; Brango, Hugo; Salcedo-Arteaga, Shirley; Hoyos-Giraldo, Luz Stella; Souza, Cláudia Telles de; Dias, Johnny Ferraz; Agudelo-Castañeda, Dayana Milena; Toscano, Ana Valdés; Gómez-Pérez, Miguel; Henriques, João Antônio Pêgas

doi:10.1016/j.ecoenv.2017.10.044

Cited by 53 publications

(26 citation statements)

References 82 publications

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“…Thus, most inhabitants around the mining zone may live in unmonitored areas or monitor-sparse locations. In a recent study, we demonstrated elevated MNBN and MNMONO frequencies in populations living in proximity to coal mining operations of northern Colombia (Espitia-Perez et al, 2017). Statistical correlation analysis suggested a strong association between MN frequencies, PM concentrations and elemental composition (particularly with some enriched elements like Cr, Cu, Zn, and S).…”

Section: Introductionmentioning

confidence: 69%

“…CBMN assay was carried out according to previously described methodology (Espitia-Perez et al, 2017). Briefly, heparinized whole blood (0.5 mL) was added to 4.5 mL of RPMI 1640 medium (Sigma R8758, USA) supplemented with 2 mM l-glutamine (Sigma G-3126, USA), 10% fetal bovine serum (Gibco/Invitrogen 15000-044, Brazil), 100 mL/mL antibiotic-antimycotic (Sigma A5955, USA) and 2% phytohemagglutinin (Sigma L8754, USA).…”

Section: Cytokinesis-block Micronucleus (Cbmn) Assaymentioning

confidence: 99%

“…For each blood sample, 2000 binucleated cells (BN) (i.e., 1000 from each of the two slides prepared from the duplicate cultures) were scored for the presence of MN (MNBN) using bright-field optical microscopy at a magnification of 200e1000Â. Additionally, MN frequency in 1.000 mononucleated cells (MONO) was evaluated to determine MNMONO frequency (Espitia-Perez et al, 2017). All slides were scored by a trained reader blinded to the exposure status of the individuals.…”

Section: Cytokinesis-block Micronucleus (Cbmn) Assaymentioning

confidence: 99%

“…To explore the relationship between the variables (PM 2.5 , PM 10 , MNBN, MNMONO and EF values), a statistical analysis was performed by the software package SPSS version 16.0. For the spatial visualization of the calculated data, a geo-statistical method was employed by using ArcGIS Software Version 10.3.1 (ESRI, Redlands, CA, USA) and previous data obtained during the sampling process for each variable (Espitia-Perez et al, 2017).…”

Section: Spatial Analysismentioning

confidence: 99%

“…Direct influence area of the mining project, open-pits, and disposal sites was georeferenced from previous reports (Espitia-Perez et al, 2017). Sampling sites (Rancherias) and surrounding coal mining areas were digitalized as reference points and used as a base layer for variable processing, analysis and spatial representation during the whole study.…”

Section: Spatial Analysismentioning

confidence: 99%

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Geospatial analysis of residential proximity to open-pit coal mining areas in relation to micronuclei frequency, particulate matter concentration, and elemental enrichment factors

et al. 2018

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During coal surface mining, several activities such as drilling, blasting, loading, and transport produce large quantities of particulate matter (PM) that is directly emitted into the atmosphere. Occupational exposure to this PM has been associated with an increase of DNA damage, but there is a scarcity of data examining the impact of these industrial operations in cytogenetic endpoints frequency and cancer risk of potentially exposed surrounding populations. In this study, we used a Geographic Information Systems (GIS) approach and Inverse Distance Weighting (IDW) methods to perform a spatial and statistical analysis to explore whether exposure to PM and PM pollution, and additional factors, including the enrichment of the PM with inorganic elements, contribute to cytogenetic damage in residents living in proximity to an open-pit coal mining area. Results showed a spatial relationship between exposure to elevated concentrations of PM PM and micronuclei frequency in binucleated (MNBN) and mononucleated (MNMONO) cells. Active pits, disposal, and storage areas could be identified as the possible emission sources of combustion elements. Mining activities were also correlated with increased concentrations of highly enriched elements like S, Cu and Cr in the atmosphere, corroborating its role in the inorganic elements pollution around coal mines. Elements enriched in the PM fraction contributed to increasing of MNBN but seems to be more related to increased MNMONO frequencies and DNA damage accumulated in vivo. The combined use of GIS and IDW methods could represent an important tool for monitoring potential cancer risk associated to dynamically distributed variables like the PM.

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Section: Introductionmentioning

confidence: 69%

Section: Cytokinesis-block Micronucleus (Cbmn) Assaymentioning

confidence: 99%

Section: Cytokinesis-block Micronucleus (Cbmn) Assaymentioning

confidence: 99%

Section: Spatial Analysismentioning

confidence: 99%

Section: Spatial Analysismentioning

confidence: 99%

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Geospatial analysis of residential proximity to open-pit coal mining areas in relation to micronuclei frequency, particulate matter concentration, and elemental enrichment factors

et al. 2018

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A review of the mineralogical and chemical composition of nanoparticles associated with coal fires

et al. 2022

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This review summarizes our current knowledge on the health and environmental impact as well as the mineralogical and geochemical composition of nanoparticles (NPs) associated with coal fires. It will furthermore recommend new sampling and characterization protocols to gain a better understanding of the various types of NPs that are formed either through high-temperature nucleation and alteration processes or via low-temperature dissolution-reprecipitation and weathering processes. Coal fires affect the immediate environment of coal-producing areas and produce positive and negative feedback to climate change through the emission of carbon-and sulfate-bearing gases and aerosols, respectively. Nanoparticles form during and after coal fires. They are composed of mainly soot and tar particles as well as amorphous phases, minerals, and complex mixtures of amorphous phases and minerals. It is recommended that NPs for mineralogical studies should be collected using impactors (a new generation of collectors for particulate matter, such as the TPS100 NP sampler) or that borosilicate filters at the openings of pipes and chambers be used to collect and measure gases emitted by coal fires. Furthermore, assemblages of NPs occurring at the mouths of coal fire vents should be examined using a combination of focused ion beam (FIB) technology and transmission electron microscopy (TEM), and those containing ion-or electron-beam sensitive phases should be examined with the corresponding cryo-techniques, such as cryo-FIB, cryo-ion mill, and cryo-TEM.The mineralogical and chemical composition of NP-bearing bulk samples should be examined with spectroscopy techniques such as X-ray photoelectron spectroscopy, 13 C nuclear magnetic resonance spectroscopy, or time-of-flight secondary ion mass spectroscopy.

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The dangerous link between coal dust exposure and DNA damage: unraveling the role of some of the chemical agents and oxidative stress

Miranda-Guevara,

Muñoz-Acevedo,

Fiorillo-Moreno

et al. 2023

Environ Geochem Health

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Exposure to coal mining dust poses a substantial health hazard to individuals due to the complex mixture of components released during the extraction process. This study aimed to assess the oxidative potential of residual coal mining dust on human lymphocyte DNA and telomeres and to perform a chemical characterization of coal dust and urine samples. The study included 150 individuals exposed to coal dust for over ten years, along with 120 control individuals. The results revealed significantly higher levels of DNA damage in the exposed group, as indicated by the standard comet assay, and oxidative damage, as determined by the FPG-modified comet assay. Moreover, the exposed individuals exhibited significantly shorter telomeres compared to the control group, and a significant correlation was found between telomere length and oxidative DNA damage. Using the PIXE method on urine samples, significantly higher concentrations of sodium (Na), phosphorus (P), sulfur (S), chlorine (Cl), potassium (K), iron (Fe), zinc (Zn), and bromine (Br) were observed in the exposed group compared to the control group. Furthermore, men showed shorter telomeres, greater DNA damage, and higher concentrations of nickel (Ni), calcium (Ca), and chromium (Cr) compared to exposed women. Additionally, the study characterized the particles released into the environment through GC–MS analysis, identifying several compounds, including polycyclic aromatic hydrocarbons (PAHs) such as fluoranthene, naphthalene, anthracene, 7H-benzo[c]fluorene, phenanthrene, pyrene, benz[a]anthracene, chrysene, and some alkyl derivatives. These findings underscore the significant health risks associated with exposure to coal mining dust, emphasizing the importance of further research and the implementation of regulatory measures to safeguard the health of individuals in affected populations. Graphic abstract

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Cytogenetic instability in populations with residential proximity to open-pit coal mine in Northern Colombia in relation to PM10 and PM2.5 levels

Cited by 53 publications

References 82 publications

Geospatial analysis of residential proximity to open-pit coal mining areas in relation to micronuclei frequency, particulate matter concentration, and elemental enrichment factors

Geospatial analysis of residential proximity to open-pit coal mining areas in relation to micronuclei frequency, particulate matter concentration, and elemental enrichment factors

A review of the mineralogical and chemical composition of nanoparticles associated with coal fires

The dangerous link between coal dust exposure and DNA damage: unraveling the role of some of the chemical agents and oxidative stress

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