Cytogenetic localization of 136 genes in the horse: comparative mapping with the human genome

Milenković, Dragan; Oustry-Vaiman, Anne; Lear, Teri L.; Billault, Alain; Mariat, Denis; Piumi, François; Schibler, Laurent; Cribiu, Edmond; Guérin, Gérard

doi:10.1007/s00335-001-2137-4

Cited by 82 publications

(96 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A specific hybridisation signal obtained by FISH using this clone as a probe was observed on the equine chromosomal region 3q22.2-q22.3 (ECA3) after examination of the metaphase spread. This result is in agreement with Zoo-FISH analyses [22] and comparative mapping data [20]. Indeed, the casein locus was localised on the 6q31 bovine chromosomal region [28], on caprine 6q32 [8] and on human 4q21.1 [17], which are orthologous of ECA3.…”

Section: Partial Characterization Of the Casein Gene Locussupporting

confidence: 90%

See 1 more Smart Citation

A specific pattern of splicing for the horse ?S1-Casein mRNA and partial genomic characterization of the relevant locus

Milenković

Martin

Guérin³

et al. 2002

Genet. Sel. Evol.

Self Cite

View full text Add to dashboard Cite

-Mares' milk has a composition very different from that of cows' milk. It is much more similar to human milk, in particular in its casein fraction. This study reports on the sequence of a 994 bp amplified fragment corresponding to a horse αS1-Casein (αS1-Cn) cDNA and its comparison with its caprine, pig, rabbit and human counterparts. The alignment of these sequences revealed a specific pattern of splicing for this horse primary transcript. As in humans, exons 3 , 6 and 13 are present whereas exons 5, 13 and 14 are absent in this equine mRNA sequence. BAC clones, screened from a horse BAC library, containing the αS1-Cn gene allowed the mapping of its locus by FISH on equine chromosome 3q22.2-q22.3 which is in agreement with the Zoo-FISH results. Genomic analysis of the αS1-Cn gene showed that the region from the second exon to the last exon is scattered within a nucleotide stretch nearly 15-kb in length which is quite similar in size to its ruminant and rabbit counterparts. The region between αS1-and β-Cn genes, suspected to contain cis-acting elements involved in the expression of all clustered casein genes, is similar in size (ca. 15-kb) to the caprine and mouse intergenic region.horse / αS1-Casein / cDNA / localisation

show abstract

Section: Partial Characterization Of the Casein Gene Locussupporting

confidence: 90%

“…To characterize the casein locus, BAC clones containing casein genes were isolated from the Inra horse BAC library [20] by PCR screening using αS1-Cn (Eq7/Eq8) and β-Cn (Eqβ1/β2) specific primer pairs.…”

Section: Genomic Analysesmentioning

confidence: 99%

A specific pattern of splicing for the horse ?S1-Casein mRNA and partial genomic characterization of the relevant locus

Milenković

Martin

Guérin³

et al. 2002

Genet. Sel. Evol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Significant strides have since been made in expanding the gene map of the horse (Equus caballus; ECA, reviewed in Chowdhary and Raudsepp 2000). Currently ∼1200 markers have been mapped/assigned to various equine chromosomes using approaches such as synteny analysis (Caetano et al 1999a,b;Shiue et al 1999), genetic linkage mapping (Lindgren et al 1998;Guérin et al 1999Guérin et al , 2003Swinburne et al 2000a), and fluorescent in situ hybridization (FISH; e.g., Raudsepp et al 1999;Godard et al 2000;Lear et al 2001;Mariat et al 2001;Milenkovic et al 2002). Among the recent prominent developments are the generation of preliminary radiation hybrid (RH) maps for chromosomes ECA1 and ECA10 (Kiguwa et al 2000) and RH and comparative maps for some of the other equine chromosomes Raudsepp et al 2002).…”

Section: Tammen]mentioning

confidence: 99%

The First-Generation Whole-Genome Radiation Hybrid Map in the Horse Identifies Conserved Segments in Human and Mouse Genomes

Chowdhary¹,

Raudsepp²,

Kata³

et al. 2003

Genome Res.

Self Cite

137

130

View full text Add to dashboard Cite

A first-generation radiation hybrid (RH) map of the equine (Equus caballus) genome was assembled using 92 horse × hamster hybrid cell lines and 730 equine markers. The map is the first comprehensive framework map of the horse that (1) incorporates type I as well as type II markers, (2) integrates synteny, cytogenetic, and meiotic maps into a consensus map, and (3) provides the most detailed genome-wide information to date on the organization and comparative status of the equine genome. The 730 loci (258 type I and 472 type II) included in the final map are clustered in 101 RH groups distributed over all equine autosomes and the X chromosome. The overall marker retention frequency in the panel is ∼21%, and the possibility of adding any new marker to the map is ∼90%. On average, the mapped markers are distributed every 19 cR (4 Mb) of the equine genome-a significant improvement in resolution over previous maps. With 69 new FISH assignments, a total of 253 cytogenetically mapped loci physically anchor the RH map to various chromosomal segments. Synteny assignments of 39 gene loci complemented the RH mapping of 27 genes. The results added 12 new loci to the horse gene map. Lastly, comparison of the assembly of 447 equine genes (256 linearly ordered RH-mapped and additional 191 FISH-mapped) with the location of draft sequences of their human and mouse orthologs provides the most extensive horse-human and horse-mouse comparative map to date. We expect that the foundation established through this map will significantly facilitate rapid targeted expansion of the horse gene map and consequently, mapping and positional cloning of genes governing traits significant to the equine industry.

show abstract

“…localized another 13 genes by FISH and somatic cell hybrids. One of the first most extensive FISH-map included 136 genes (MILENKOVIC et al, 2002), and to this cytogenetic map further 165 genes were added by FISH and/or radiation hybrid (RH) mapping (PERROCHEAU et al, 2005). The equine gene map now contains 713 genes, 441 on the RH map, 511 on the cytogenetic and 238 on both maps.…”

Section: Cytogenetic Mapmentioning

confidence: 99%

Mapping the horse genome and its impact on equine genomics for identification of genes for monogenic and complex traits – a review

Stübs

Distl

2007

Arch. Anim. Breed.

View full text Add to dashboard Cite

Since the beginning of investigation in the horse genome in the early nineties, there has been a great progress, especially during the last five years. At the beginning the exploration of monogenic hereditary diseases was one of the main aims, and the causal mutations of several diseases in the horse have been unravelled. The inheritance of coat colours has been explored very detailed, and there exist gene tests for different coat colours. Information about coat colours and inherited diseases is very important for the breeders and helps avoiding the appearance of lethal genetic factors or undesirable diseases. The most important achievements of horse genome analysis were well-developed linkage, radiation hybrid and cytogenetic genome maps including more than 2950 loci. These maps support comparative analysis of equine hereditary diseases. The present known gene mutations for five diseases in horses have human homologs. Studies on multifactorial diseases such as osteochondrosis and navicular bone disease and on fertility and temperament are underway. At the moment, the whole equine genome is sequenced as it has been done for the human genome and also for other animal genomes. Horse breeding will greatly benefit from identification of QTL for multifactorial traits and gene mutations for congenital anomalies, diseases and performance traits.

show abstract

Cytogenetic localization of 136 genes in the horse: comparative mapping with the human genome

Cited by 82 publications

References 0 publications

A specific pattern of splicing for the horse ?S1-Casein mRNA and partial genomic characterization of the relevant locus

A specific pattern of splicing for the horse ?S1-Casein mRNA and partial genomic characterization of the relevant locus

The First-Generation Whole-Genome Radiation Hybrid Map in the Horse Identifies Conserved Segments in Human and Mouse Genomes

Mapping the horse genome and its impact on equine genomics for identification of genes for monogenic and complex traits – a review

Contact Info

Product

Resources

About