Cytogenetic mapping of immunoglobulin heavy chain genes in Antarctic fish

Pisano, Eva; Coscia, Maria Rosaria; Mazzei, F.; Ghigliotti, Laura; Coutanceau, Jean-Pierre; Ozouf−Costaz, Catherine; Oreste, Umberto

doi:10.1007/s10709-006-0015-4

Cited by 13 publications

(8 citation statements)

References 31 publications

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“…More interestingly, SINE elements found in the third intron of T. bernacchii rep-resented the highest percentage (7.3 %) of TEs identified in the other cold adapted species. It is well known that SINEs are frequently found in Trematominae genus, accounting for their rapid radiation/genome rearrangement [40,41]. To our knowledge this finding is not surprising.…”

Section: Discussionsupporting

confidence: 51%

Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Revealed Distinctive Features of Cold Adapted Notothenioidei

Ametrano¹,

Picchietti²,

Guerra³

et al. 2022

Preprint

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Previously, we identified and characterized the IgM and IgT classes in the Antarctic teleost Trematomus bernacchii, a species belonging to the Perciform suborder Notothenoidei. Herein we characterized the gene encoding the polymeric immunoglobulin receptor (pIgR) in the same species and compared it to pIgR of multiple teleost species belonging to five perciform suborders, including 11 Antarctic and one non-Antarctic (Cottoperca gobio) notothenioid species, the latter living in less cold periantarctic sea. Antarctic pIgR genes displayed particularly long introns marked by sites of transposable elements and transcription factors. Furthermore, analysis of T. bernacchii pIgR cDNA unveiled multiple amino acid substitutions unique to Antarctic species, all introducing adaptive features, including N-glycosylation sequons. Interestingly, C. gobio shared most features with the other perciforms rather than with the cold adapted relatives. T. bernacchii pIgR transcripts were predominantly expressed in mucosal tissues, as indicated by q-PCR and in situ hybridization analysis. These results suggest that in cold adapted species pIgR preserved its fundamental role in mucosal immune defense, although remarkable gene structure modifications occurred.

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Section: Discussionsupporting

confidence: 51%

Comparative Analysis of the pIgR Gene from the Antarctic Teleost Trematomus bernacchii Revealed Distinctive Features of Cold Adapted Notothenioidei

Ametrano¹,

Picchietti²,

Guerra³

et al. 2022

Preprint

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“…Interestingly, we could not identify any variant characterized, like the Sts isoform of T. bernacchii, by the skipping of the Cτ2 exon remnant. Therefore, Sts may be considered a species-specific isoform, possibly linked with the significant genomic reorganization of Trematominae[57]. Most likely, the availability of additional fully sequenced notothenioid genomes will clarify the orthology and paralogy relationships of the different sequence variants identified in this work.…”

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confidence: 93%

Fitting Together the Evolutionary Puzzle pieces of the Immunoglobulin T Gene from Antarctic Fishes

Ametrano¹,

Gerdol²,

Vitale³

et al. 2020

Preprint

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Cryonotothenioidea is the main group of fishes that thrive in the extremely cold Antarctic environment, thanks to the acquisition of peculiar morphological, physiological and molecular adaptations. We have previously disclosed that IgM, the main immunoglobulin isotype in teleosts, display typical cold-adapted features. Recently, we have analyzed the gene encoding the heavy chain constant region (CH) of the IgT isotype from the Antarctic teleost Trematomus bernacchii (family Nototheniidae), characterized by the near-complete deletion of the CH2 domain. Here, we aimed to track the loss of the CH2 domain along notothenioid phylogeny and to identify its ancestral origins. To this end, we obtained the IgT gene sequences from several species belonging to the Antarctic families Nototheniidae, Bathydraconidae and Artedidraconidae. All species display a CH2 remnant of variable size, encoded by a short Cτ2 exon, which retains functional splicing sites and therefore is included in the mature transcript. We also considered representative species from the three non-Antarctic families: Eleginopsioidea (Eleginops maclovinus), Pseudaphritioidea (Pseudaphritis urvillii) and Bovichtidae (Bovichtus diacanthus and Cottoperca gobio). Even though only E. maclovinus, the sister taxa of Cryonotothenioidea, shared the partial loss of Cτ2, the other non-Antarctic notothenioid species displayed early molecular signatures of this event. These results shed light on the evolutionary path that underlies the origins of this remarkable gene structural modification.

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“…The duplication of the IgH locus has been documented also by the cytogenetic mapping on twelve notothenioid species: four species, all belonging to the Trematomus genus, showed hybridization signals on two different chromosome pairs (Pisano et al, 2007).…”

Section: Genomic Structure Of the Igh Locusmentioning

confidence: 88%

“…In addition, the presence of three allelic cDNA IgH sequences in one T. newnesi specimen indicated the occurrence of two functional loci in this species (Pisano et al, 2007).…”

Section: Secreted Ig Heavy Chainmentioning

confidence: 91%