Cytogenetic mapping of the Lethal hybrid rescue gene of Drosophila simulans.

Yamamoto, Masatoshi; Kamo, Masaki; Yamamoto, Seigo; Watanabe, Takao

doi:10.1266/ggs.72.297

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…The discrepancy with the previous report in 54E-F (Yamamoto et al, 1997) is largely due to the precise mapping of sd in 50D in the present study.…”

Section: Discussioncontrasting

confidence: 99%

“…The Lhr mutation was previously mapped between sd and jba (Yamamoto et al, 1997). The corresponding genes for sd and jba have not been identified in D. melanogaster and their position is cytologically uncertain.…”

Section: Genetic Mapping Of Sd Jba and Lhrmentioning

confidence: 99%

“…1A-B). We took advantage of a previously identified CyO balancer chromosome that should carry a sd mutation of D. melanogaster (Yamamoto et al, 1997) to map the sd gene of both species. The location of sd turned out to be in 50D4-50D7 (Fig.…”

Section: Genetic Mapping Of Sd Jba and Lhrmentioning

confidence: 99%

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Transgenic Drosophila simulans strains prove the identity of the speciation gene Lethal hybrid rescue

2009

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Speciation genes are responsible for genetic incompatibilities in hybrids of incipient species and therefore participate in reproductive isolation leading to complete speciation. Hybrid males between Drosophila melanogaster females and D. simulans males die at late larval or prepupal stages due to a failure in chromosome condensation during mitosis. However a mutant male of D. simulans, named Lethal hybrid rescue (Lhr), produces viable hybrid males when crossed to females of D. melanogaster. Recently the Lhr gene has been proposed as corresponding to the CG18468 gene in D. melanogaster. However this identification relied on sequence characteristics more than on a precise mapping and the use of the GAL4/UAS system to drive the transgene in D. melanogaster might have increased the complexity of interaction. Thus here we propose an independent identification of the Lhr gene based on a more precise mapping and transgenic experiments in D. simulans. We have mapped the Lhr gene by using Single Nucleotide Polymorphisms (SNPs) and identified within the candidate region the gene homologous to CG18468 as the Lhr gene as it was previously reported. Transgenic experiments in D. simulans with the native promoter of CG18468 prove that it is the Lhr gene of D. simulans by inducing the lethality of the hybrid males.

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“…The discrepancy with the previous report in 54E-F (Yamamoto et al, 1997) is largely due to the precise mapping of sd in 50D in the present study.…”

Section: Discussioncontrasting

confidence: 99%

Section: Genetic Mapping Of Sd Jba and Lhrmentioning

confidence: 99%

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Transgenic Drosophila simulans strains prove the identity of the speciation gene Lethal hybrid rescue

2009

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“…While T. K. Watanabe and colleagues were surveying the invading population of the species in the late 1970s, Watanabe (1979) noticed that males of a strain from Kokura ‘rescued’ the inviable hybrid males when mated with D. melanogaster females. The rescue gene, Lethal hybrid rescue ( Lhr ), has been mapped in the right arm of the second chromosome by both genetical and cytological means (Watanabe 1979; Yamamoto et al 1997). The rescue effect of Lhr is not sex‐specific, because X m X m Y s females are also rescuable by this mutation (Takamura & Watanabe 1980).…”

Section: Introductionmentioning

confidence: 99%

Genetics of hybrid inviability and sterility in Drosophila: The Drosophila melanogaster–Drosophila simulans case

Sawamura

2000

Plant Species Biology

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How reproductive isolation is established between populations is a fundamental question in the study of speciation. To answer this question, mechanisms of prezygotic and postzygotic isolation have been analyzed in many species pairs from diverse taxa. Because of its genetic manipulability, the genus Drosophila is suitable for such analyses. Since hybrid inviability and sterility was noticed in crosses between Drosophila melanogaster and Drosophila simulans 90 years ago, this species pair has contributed much to our knowledge of genetic mechanisms of reproductive isolation. The history of genetic analyses of postzygotic isolation in this species pair is reviewed here.

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“…Lhr 1 maps 1.7 centimorgans from the visible marker jba on chromosome 2R (17). We identified recombinants between Lhr 1 and jba and typed them using molecular markers that distinguish the Lhr 1 and jba D. simulans strains (Fig.…”

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confidence: 99%

Two Dobzhansky-Muller Genes Interact to Cause Hybrid Lethality in Drosophila

Brideau

Flores

Wang

et al. 2006

Science

369

440

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The Dobzhansky-Muller model proposes that hybrid incompatibilities are caused by the interaction between genes that have functionally diverged in the respective hybridizing species. Here, we show that Lethal hybrid rescue (Lhr) has functionally diverged in Drosophila simulans and interacts with Hybrid male rescue (Hmr), which has functionally diverged in D. melanogaster, to cause lethality in F1 hybrid males. LHR localizes to heterochromatic regions of the genome and has diverged extensively in sequence between these species in a manner consistent with positive selection. Rapidly evolving heterochromatic DNA sequences may be driving the evolution of this incompatibility gene.

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Cytogenetic mapping of the Lethal hybrid rescue gene of Drosophila simulans.

Cited by 6 publications

References 14 publications

Transgenic Drosophila simulans strains prove the identity of the speciation gene Lethal hybrid rescue

Transgenic Drosophila simulans strains prove the identity of the speciation gene Lethal hybrid rescue

Genetics of hybrid inviability and sterility in Drosophila: The Drosophila melanogaster–Drosophila simulans case

Two Dobzhansky-Muller Genes Interact to Cause Hybrid Lethality in Drosophila

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