Cytogenetic monitoring by use of the micronucleus assay among hospital workers exposed to low doses of ionizing radiation

Sari-Minodier, I.; Orsière, Thierry; Auquier, P.; Martín, F. de Juan; Botta, A.

doi:10.1016/j.mrgentox.2007.01.009

Cited by 65 publications

(61 citation statements)

References 51 publications

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“…In the entire studied population, men had a higher NPB frequency, as in another study on healthy volunteers (29), but the knowledge of the effect of confounding factor on NPBs frequencies is not as extensive as on MNi. Generally, the effects of smoking on DNA damage in individuals exposed to IR are still unclear, with opposite results (9,20,24,26,30,31). In IR exposed subjects, smoking habits were associated with increased MNi frequency, which is in agreement with previous studies (20,30), but there was a significantly decreased MNi frequency in the control group.…”

Section: Discussionsupporting

confidence: 85%

“…From this point of view, the years the workers were occupationally exposed to IR may represent an attempt to overcome the limits associated with the use of dosimeters. Positive correlation of MNi frequency and exposure duration has been confirmed in previous studies (23,26), with a reported elevated high risk of cancer in medical X-ray workers as their service duration increased (2). Our IR exposed group demonstrated a clear relation between MNi and NB frequencies, that is, an increase of 0.4 and 0.07 in the number of MNi and NBs respectively, per 1 year of IR occupational exposure.…”

Section: Discussionsupporting

confidence: 66%

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Polymorphisms in DNA repair genes: link with biomarkers of the CBMN cytome assay in hospital workers chronically exposed to low doses of ionising radiation / Polimorfizmi u genima za popravak DNA: poveznica s biomarkerima mikronukleus-testa u medicinskih radnika kronično izloženih niskim dozama ionizirajućeg zračenja

Milić

Rozgaj

Kašuba

et al. 2015

Archives of Industrial Hygiene and Toxicology

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Individual sensitivity to ionising radiation (IR) is the result of interaction between exposure, DNA damage, and its repair, which is why polymorphisms in DNA repair genes could play an important role. We examined the association between DNA damage, expressed as micronuclei (MNi), nuclear buds (NBs), and nucleoplasmic bridges (NPBs) and single nucleotide polymorphisms in selected DNA repair genes (APE1, hOGG1, XRCC1, XRCC3, XPD, PARP1, MGMT genes; representative of the different DNA repair pathways operating in mammals) in 77 hospital workers chronically exposed to low doses of IR, and 70 matched controls. A significantly higher MNi frequency was found in the exposed group (16.2±10.4 vs. 11.5±9.4; P=0.003) and the effect appeared to be independent from the principal confounding factor. Exposed individuals with hOGG1, XRCC1, PARP1, and MGMT wild-type alleles or APEX1, as well as XPD (rs13181) heterozygous showed a significantly higher MNi frequency than controls with the same genotypes. Genetic polymorphism analysis and cytogenetic dosimetry have proven to be a powerful tool complementary to physical dosimetry in regular health surveillance programmes.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 66%

Polymorphisms in DNA repair genes: link with biomarkers of the CBMN cytome assay in hospital workers chronically exposed to low doses of ionising radiation / Polimorfizmi u genima za popravak DNA: poveznica s biomarkerima mikronukleus-testa u medicinskih radnika kronično izloženih niskim dozama ionizirajućeg zračenja

Milić

Rozgaj

Kašuba

et al. 2015

Archives of Industrial Hygiene and Toxicology

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“…It has been also shown that a higher MN frequency observed with ageing is directly associated with decreased efficiency of DNA repair and increased genomic instability [28]. Contrary to the study of Sari-Minodier et al [3], the influence of age and gender on the MNs frequency was not further investigated due to the relatively small size of the present study population, which could decrease the statistical power. We were unable to find an influence of general and dermic symptoms on the levels of all the studied biomarkers, either.…”

Section: Discussioncontrasting

confidence: 55%

“…The levels of exposure to ionizing radiation in hospitals have decreased in recent decades and are now far below the regulatory limit of 20 mSv/year, indeed below the detection limit of dosimeters. Some medical uses of radiation, such as nuclear medicine and interventional procedures, may expose the personnel to higher doses, and these are subjects of concern [3]. Recently, Engin et al [4] have assessed the genomic instability in γ-radiation and X-ray-exposed hospital staff and observed that chronic exposure to low levels of IR, below the accepted limit, could induce oxidative stress and increased apoptosis frequency, compared to the nonexposed staff.…”

Section: Subjectsmentioning

confidence: 99%

A cytogenetic approach to the effects of low levels of ionizing radiation (IR) on the exposed Tunisian hospital workers

Bouraoui¹,

Mougou²,

Drira³

et al. 2013

International Journal of Occupational Medicine and Environmental Health

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Objectives: The aim of this study is to assess chromosomal damage in Tunisian hospital workers occupationally exposed to low levels of ionizing radiation (IR). Materials and Methods: The cytokinesis-block micronucleus (CBMN) assay in the peripheral lymphocytes of 67 exposed workers compared to 43 controls matched for gender, age and smoking habits was used. The clastogenic/aneugenic effect of IR was evaluated using the CBMN assay in combination with fluorescence in situ hybridization with human pan-centromeric DNA in all the exposed subjects and controls. Results: The study showed a significant increase of the micronucleus (MN) frequency in the lymphocytes of the exposed workers compared to the control group (13.63±4.9‰ vs. 6.52±4.21‰, p < 0.05). The centromere analysis performed in our study showed that MNs in hospital staff were predominantly centromere negative (72%) and the mean negative labeled micronucleus (C-MN) frequency was significantly higher in the exposed subjects than in the controls (9.04±4.57‰ vs. 1.17±0.77‰). The multivariate regression analysis, taking into account all confounding factors, showed that only the time of exposure to IR had a significant effect on the level of MNs and C-MN. Conclusion: The present study shows that chromosomal damage leading to the formation of micronucleated lymphocytes is more frequent in the hospital workers exposed to IR than in the controls, despite the low levels of exposure. The results of the study confirm the well-known clastogenic properties of ionizing radiation. In regards to health monitoring, detection of early genotoxic effects may allow for the adoption of preventive biological control measures, such as hygienic improvements in the workplace or reduction of hours of occupational exposure.

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“…At DNA level, IR exerts its genotoxic effect by the induction of singleand double-strand breaks, a-basic sites and oxidized bases. Moreover, the exposure to IR has been associated with gene mutations, amplification of genetic material, chromosomal rearrangements and increased frequencies of apoptosis, chromosomal aberrations (CAs), micronuclei and sister chromatid exchanges (SCEs) (Lazutka et al, 1999;Limoli et al, 2000;Engin et al, 2005;Dias et al, 2007;Sari-Minodier et al, 2007;Sakly et al, 2012a,b). These genotoxic effects of the radiations would result in each individual in the induction of a carcinogenic process (Breimer, 1988;Overbeek et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Increased frequency of chromosomal aberrations and sister chromatid exchanges in peripheral lymphocytes of radiology technicians chronically exposed to low levels of ionizing radiations

Santovito

Cervella

Delpero

2014

Environmental Toxicology and Pharmacology

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Chromosome aberrations (CAs) and sister chromatid exchanges (SCEs) frequencies were estimated in peripheral lymphocytes from 21 radiology technicians, and from 21 nonexposed control subjects. We exclusively considered individuals who neither smoke nor consume drugs or alcohol for a period of at least two years prior to the analysis. Significant differences were found between exposed and controls in terms of SCEs and CAs frequencies. Technicians showed a significant higher number of high-frequency individuals (HFIs) with respect to the control group. Nevertheless, the mean frequency of SCEs observed among technician HFIs did not significantly differ with respect to that observed among control HFIs. Vice versa, the nonHFIs belonging to technicians group showed a statistically higher difference in the SCEs/NSM value with respect to the non-HFIs belonging to control group. Since the differences in the SCEs frequencies between the two groups are due to non-HFIs, our results seem to indicate a general genotoxic effect of the IR, not affected by HFIs. Among technicians, the level of chromosome damage correlated neither with years of radiation exposure nor with the age of the subjects. Vice versa, in the control group, a positive correlation was found between the number of SCEs and age. In both samples the gender status did not influence the frequencies of CAs and SCEs. Our results suggest that chronic long-term exposure to low doses of ionizing radiation could increase the CAs and SCEs frequencies. This study reinforces the relevance of the biomonitoring of hospital workers chronically exposed to ionizing radiation.

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Cytogenetic monitoring by use of the micronucleus assay among hospital workers exposed to low doses of ionizing radiation

Cited by 65 publications

References 51 publications

A cytogenetic approach to the effects of low levels of ionizing radiation (IR) on the exposed Tunisian hospital workers

Increased frequency of chromosomal aberrations and sister chromatid exchanges in peripheral lymphocytes of radiology technicians chronically exposed to low levels of ionizing radiations

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