Cytogenetic monitoring of farm animals under conditions of environmental pollution

Rubeš, Jiřı́; Borkovec, L.; Hořínová, Z.; Urbanová, J.; Proroková, I; Kulikova, L. S.

doi:10.1016/0165-7992(92)90108-t

Cited by 22 publications

(10 citation statements)

References 10 publications

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“…The percent of aberrations in 100 cells shown in Table 2 can be also expressed as break per cell values (100 times lower, respectively), applied often to evaluate genotoxic consequences of environmental factors. The results obtained for control groups corresponds to known effects of environment with minimum contamination, whereas the parameters found for test groups can be compared with those defined for farm animals populations under conditions of chemical pollution (Rubeš et al, 1992). The differences between groups studied, in spite of the same of 5-AZA and BrdU dosage used for in vitro experiment result probably from not even individual ability to repair genome structure damages.…”

Section: Resultsmentioning

confidence: 99%

Mutagen-induced chromosome instability in farm animals

Danielak-Czech¹,

Słota²

2004

J. Anim. Feed Sci.

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The frequencies of spontaneous and in vitro mutagen-induced unstable chromosomal lesions were compared in the groups of subfertile cows, ewes and sows (test groups) as well as the animals without any reproductive disturbances (control groups). The mean percentage of 5-azacytidineand bromodeoxyuridine-induced defects differed statistically between the test and control groups (P≤0.01 in cattle, P≤0.05 in sheep and pigs). In the groups of animals with reproductive problems the number of defects observed in some chromosomes exceeded the expected values (calculated according to the relative chromosome length), but only in the case of X chromosome differed at the level of statistical significance. Moreover, the groups of cows analysed differed significantly as regards the number of chromatid breaks/gaps at the Xq2.4 and Xq3.1 fragile sites. The excessive chromosome instability in subfertile farm animals, particularly the X-specific loci, may result in inappropriate expression of genes determining reproductive traits as well as in the increased rate of aneuploid germ cells related to prenatal and perinatal mortality.

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Section: Resultsmentioning

confidence: 99%

Mutagen-induced chromosome instability in farm animals

Danielak-Czech¹,

Słota²

2004

J. Anim. Feed Sci.

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“…Studies of the horse genome and karyotype have been undertaken to analyse their evolutionary aspect and compare them with the genomes of other mammal species (CAE-TANO et al 1999;WADE et al 2009). Few publications address chromosome instability in horses (RUBES et al 1992;WÓJCIK et al 2011) and cytogenetic analyses undertaken by scientists mainly concern irregularities in chromosome number or structure (BUGNO & S£OTA 2007;LEAR & BAI-LEY 2008;DI MEO et al 2009).…”

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confidence: 99%

The Effect of Environmental Factors on Sister Chromatid Exchange Incidence in Domestic Horse (Equus caballus) Chromosomes

Wójcik

Smalec²

2013

folia biol (krakow)

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The SCE test is often used as a sensitive and reliable technique in the biomonitoring of genotoxicity of mutagenic and carcinogenic agents. This study analysed the frequency of sister chromatid exchange in domestic horse chromosomes depending on the habitat and age of the analysed horses. The chromosome preparations were obtained from an in vitro culture of peripheral blood lymphocytes stained using the FPG technique. Both the habitat and the age significantly influence SCE frequency. A higher SCE incidence was observed in horses that lived in a large urban agglomeration than in those from the country. Also, a higher SCE incidence was identified in the group of horses above 6 years of age in comparison with the younger ones. Additionally, the frequency of SCEs in the first, second and third chromosomes and the X sex chromosome were analysed in detail. More exposed to the effect of environmental pollutants, the horses from the urban environment developed more double and triple SCEs in comparison with the village horses. The urban horses also developed quadruple SCEs, in addition to the less frequent exchanges.

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“…The cytogenetic investigations that have been undertaken primarily concerned inadequate number (aneuploidies) or structure (translocations) of chromosomes (Bugno & Słota 2007, Lear & Bailey 2008). There are few reports available on chromosome instability in horses (Rubes et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Sister chromatid exchange in selected horse breeds (<i>Equus caballus</i>)

2011

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In studies of chromosome instability, the sister chromatid exchange (SCE) test is a particularly sensitive cytogenetic assay for detecting DNA damage. SCE tests of chromosome instability were performed in the group of 6 horse breeds (Pure-bred Arabian, Malapolski horse, Polish noble half-bred, Polish cold-blooded, Hucul and Polish Konik). The chromosome preparations were obtained from our in vitro culture of peripheral blood lymphocytes stained using the FPG technique. The mean number of SCEs/cell in the analysed population of horses was 5.14±1.44. The mean frequency of SCEs in the 6 analysed horse breeds varied depending on the breed. Statistically significant differences were observed between the horse breeds (P<0.01).No statistically significant differences in the number of SCEs per cell were found between the males and females (5.10±1.34 and 5.20±1.52, respectively). The horses were also assessed for the number of SCEs/cell in relation to the age of the animals. The differences between the age groups were statistically significant (P<0.01).

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Cytogenetic monitoring of farm animals under conditions of environmental pollution

Cited by 22 publications

References 10 publications

Mutagen-induced chromosome instability in farm animals

Mutagen-induced chromosome instability in farm animals

The Effect of Environmental Factors on Sister Chromatid Exchange Incidence in Domestic Horse (Equus caballus) Chromosomes

Sister chromatid exchange in selected horse breeds (<i>Equus caballus</i>)

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Cytogenetic monitoring of farm animals under conditions of environmental pollution

Cited by 22 publications

References 10 publications

Mutagen-induced chromosome instability in farm animals

Mutagen-induced chromosome instability in farm animals

The Effect of Environmental Factors on Sister Chromatid Exchange Incidence in Domestic Horse (Equus caballus) Chromosomes

Sister chromatid exchange in selected horse breeds (&lt;i&gt;Equus caballus&lt;/i&gt;)

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Sister chromatid exchange in selected horse breeds (<i>Equus caballus</i>)