Cytogenetic monitoring of industrial radiographers using the micronucleus assay

Sari-Minodier, I.; Orsière, Thierry; Bellon, L.; Pompili, J.; Sapin, Christophe; Botta, A.

doi:10.1016/s1383-5718(02)00213-9

Cited by 56 publications

(27 citation statements)

References 42 publications

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“…9,16,28 The MNC frequencies were not significantly different before and after X-ray exposure in our sample. These results contrast with those of other authors, 2,12,16,29,30 who reported higher rates of chromosomal aberrations subsequent to X-ray exposure. However, despite higher radiation doses in our investigation, research with similar methodology with dental radiographs showed similar results compared to our study (ie, no mutagenic characteristic was evidenced by the MN test).…”

Section: Discussioncontrasting

confidence: 99%

Cytogenetic biomonitoring in children submitting to a complete set of radiographs for orthodontic planning

Lorenzoni¹,

Fracalossi²,

Carlin³

et al. 2012

The Angle Orthodontist

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Objectives: To evaluate the DNA damage (micronucleus) and cellular death (pyknosis, karyolysis, and karyorrhexis) in exfoliated buccal mucosa cells from children undergoing orthodontic radiographs. Materials and Methods: A total of 25 healthy children undergoing orthodontic therapy partook in a complete set of orthodontic radiographs (lateral cephalographic, posteroanterior cephalographic, panoramic, full periapical exam, and bitewing). The micronucleus test in the buccal exfoliated cells was applied. The paired-samples t-test and the Wilcoxon test were used to compare the frequencies of alterations before and after X-ray exposure. Results: We found no statistically significant differences (P . .05) between micronucleated buccal mucosa cells before and after exposure to radiation. However, radiation did cause other nuclear alterations closely related to cytotoxicity (P 5 .007). Conclusion: According to the micronucleus test, the complete set of radiographs requested in the orthodontic planning may not be a factor that induces chromosomal damage, but it is able to promote cytotoxicity. (Angle Orthod. 2012;82:585-590.)

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Section: Discussioncontrasting

confidence: 99%

Cytogenetic biomonitoring in children submitting to a complete set of radiographs for orthodontic planning

Lorenzoni¹,

Fracalossi²,

Carlin³

et al. 2012

The Angle Orthodontist

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“…Vral et al [10] reported a high percentage of spontaneous MNs containing centromeres, while mainly centromere negative MNs (C-MN) were induced after irradiation with low doses of 6°CO γ-ray. Sari-Minodier et al [13] also showed that chromosomal damage leading to the formation of micronucleated lymphocytes in industrial radiographers was due to a markedly higher frequency of C-MN. In a study of miners who were chronically exposed to radionucleotides found in uranium mines, Kryscio et al [24] reported that the percentage of centromere positive MNs (C+MN) was lower in the miners than in the controls.…”

Section: Discussionmentioning

confidence: 97%

“…The levels of exposure to IR in hospitals have decreased in recent decades, and are now below the regularity limit of 20 mSv/year, and even below the detection limit of dosimeters [16]. In Tunisia, the threshold dose of permissible radiation is limited to 50 mSv/year for the exposed workers.…”

Section: Discussionmentioning

confidence: 99%

“…Using heparinized vacutainer tubes, 5 ml samples of whole blood were obtained and kept at room temperature for less than 6 h. The CBMN assay was performed as described by Sari-Minodier et al [13]. The cultures were started by adding 0.7 ml of whole blood to 9.3 ml of Roswell Park Memorial Institute medium (RPMI) supplemented with 25% fetal bovine serum, heparin (50 U/ml), antibiotics (penicillin 100 U/ml and streptomycin 100 μg/ml) and 1% PHA and kept for 72 h in a humidified CO 2 incubator at 37°C.…”

Section: Cytokinesis-blocked Micronucleus Assay (Cbmn)mentioning

confidence: 99%

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A cytogenetic approach to the effects of low levels of ionizing radiation (IR) on the exposed Tunisian hospital workers

Bouraoui¹,

Mougou²,

Drira³

et al. 2013

International Journal of Occupational Medicine and Environmental Health

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Objectives: The aim of this study is to assess chromosomal damage in Tunisian hospital workers occupationally exposed to low levels of ionizing radiation (IR). Materials and Methods: The cytokinesis-block micronucleus (CBMN) assay in the peripheral lymphocytes of 67 exposed workers compared to 43 controls matched for gender, age and smoking habits was used. The clastogenic/aneugenic effect of IR was evaluated using the CBMN assay in combination with fluorescence in situ hybridization with human pan-centromeric DNA in all the exposed subjects and controls. Results: The study showed a significant increase of the micronucleus (MN) frequency in the lymphocytes of the exposed workers compared to the control group (13.63±4.9‰ vs. 6.52±4.21‰, p < 0.05). The centromere analysis performed in our study showed that MNs in hospital staff were predominantly centromere negative (72%) and the mean negative labeled micronucleus (C-MN) frequency was significantly higher in the exposed subjects than in the controls (9.04±4.57‰ vs. 1.17±0.77‰). The multivariate regression analysis, taking into account all confounding factors, showed that only the time of exposure to IR had a significant effect on the level of MNs and C-MN. Conclusion: The present study shows that chromosomal damage leading to the formation of micronucleated lymphocytes is more frequent in the hospital workers exposed to IR than in the controls, despite the low levels of exposure. The results of the study confirm the well-known clastogenic properties of ionizing radiation. In regards to health monitoring, detection of early genotoxic effects may allow for the adoption of preventive biological control measures, such as hygienic improvements in the workplace or reduction of hours of occupational exposure.

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“…The MN are formed from the chromosomal fragments or entire chromosomes that are not incorporated into the nucleus of the daughter cell during cell division, they are corpuscles containing DNA without structural connection to the core. The presence of MN and nuclear abnormalities can be considered as a preliminary indication for assessing the mutagenic and/or carcinogenic agents, such as ionizing radiation (Sari-Minodier et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Toxicogenetic biomonitoring of occupational risk induced by ionizing radiation

Ronald¹,

Torequl²,

Ricardo³

et al. 2016

Afr. J. Pharm. Pharmacol.

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Occupational exposure to ionizing radiation (IR) can cause systemic acute and chronic effects on human health, including genetic instability that may be etiology of various diseases, including cancer. The aim of this study was to evaluate the possible toxicogenetic changes in haematological and biochemical parameters, and cytogenetic biomarkers (micronuclei and nuclear abnormalities) indicators of mutagenicity and apoptosis, as well as seek their correlation with lifestyle, age and gender. In accordance with the ethical aspects, 45 professionals (technicians and technologists in radiology) occupationally exposed to low doses of IR participated in this study. For control, 45 healthy individuals were not exposed to IR and/or genotoxic chemicals were included. Peripheral blood and oral epithelium samples were used in the toxic evaluations. The results suggested unchanged hematological biomarkers but a significant (P < 0.05) increases in the frequency of micronuclei, sprouts, binucleate cells and bridges, as well as karyolysis and karyorrhexis in professional radiology sector. Hepatic and nephritic toxicity were not observed. Without protection, a significant (P < 0.01) correlation (P < 0.05) was observed between toxicogenetic biomarkers with age, smoking, alcohol consumption, time and place of work. In conclusion, IR may be associated with genetic instability in health diseases, like cancer.

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Cytogenetic monitoring of industrial radiographers using the micronucleus assay

Cited by 56 publications

References 42 publications

Cytogenetic biomonitoring in children submitting to a complete set of radiographs for orthodontic planning

Cytogenetic biomonitoring in children submitting to a complete set of radiographs for orthodontic planning

A cytogenetic approach to the effects of low levels of ionizing radiation (IR) on the exposed Tunisian hospital workers

Toxicogenetic biomonitoring of occupational risk induced by ionizing radiation

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