Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: Standard and Fpg-modified comet assay

Gajski, Goran; Garaj‐Vrhovac, Vera; Oreščanin, Višnja

doi:10.1016/j.taap.2008.03.013

Cited by 66 publications

(37 citation statements)

References 59 publications

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“…Due to its DNA intercalation property, E + is also used in techniques to differentiate necrotic from apoptotic cell death [25]. Recent studies indicate that the product resultant from the reaction of HE with O 2…”

Section: Hydroethidine (Dihydroethidium; He)mentioning

confidence: 99%

Optical probes for detection and quantification of neutrophils’ oxidative burst. A review

Freitas

Lima

Fernandes

2009

Analytica Chimica Acta

152

115

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Section: Hydroethidine (Dihydroethidium; He)mentioning

confidence: 99%

Optical probes for detection and quantification of neutrophils’ oxidative burst. A review

Freitas

Lima

Fernandes

2009

Analytica Chimica Acta

152

115

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“…Single cells can be used in in vivo and in vitro as well as in biomonitoring of population exposed to radiation or chemical mutagens. 6,18,19 The comet assay detects single and double stranded breaks at the level of DNA molecule, sites of incomplete repair, alkali labile sites, DNA-DNA and DNAprotein cross-links. Besides, the comet assay can be used for detection of the level of the DNA fragmentation in apoptosis.…”

Section: Exposure Conditionsmentioning

confidence: 99%

Usage of the standard and modified comet assay in assessment of DNA damage in human lymphocytes after exposure to ionizing radiation

Mikloš¹,

Gajski²,

Garaj‐Vrhovac³

2009

Radiology and Oncology

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“…The comet assay and micronucleus test are fast and effective tools for assessing the primary toxic effects induced by chemical and physical agents (20)(21)(22). The comet assay is a rapid, sensitive, and relatively simple method for detecting DNA damage at the level of individual cell.…”

mentioning

confidence: 99%

Evaluation of the Cytogenetic Status of Human Lymphocytes After Exposure to a High Concentration of Bee Venom In Vitro

Garaj-Vrhovac

Gajski

2009

Archives of Industrial Hygiene and Toxicology

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Several studies have reported radioprotective, antimutagenic, anti-infl ammatory, antinociceptive, and anticancer effects of bee venom both in the cell and the whole organism. The aim of this study was to assess the effects of a single high dose of 100 µg mL -1 of whole bee venom in human lymphocytes in vitro over a variety of time spans (from 10 min to 24 h). After the treatment, we used the comet assay and micronucleus test to see the effect of bee venom on the cell. The comet assay confi rmed that the venom damaged the DNA molecule. Tail length, tail intensity, tail moment showed a signifi cant increase (P<0.05). The percentage of long-tailed nuclei (LTN) with the tail length exceeding the 95 th percentile also increased in a time-dependent manner. The micronucleus parameters (number of micronuclei, nucleoplasmic bridges, and nuclear buds) also showed a signifi cant time-dependent increase (P<0.05). This research indicates that high concentrations of bee venom can lead to cellular instability. Further research is needed to understand the mechanism of action of bee venom and its components in human cells and to see if this natural product may fi nd application in medicine.

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Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: Standard and Fpg-modified comet assay

Cited by 66 publications

References 59 publications

Optical probes for detection and quantification of neutrophils’ oxidative burst. A review

Optical probes for detection and quantification of neutrophils’ oxidative burst. A review

Usage of the standard and modified comet assay in assessment of DNA damage in human lymphocytes after exposure to ionizing radiation

Evaluation of the Cytogenetic Status of Human Lymphocytes After Exposure to a High Concentration of Bee Venom In Vitro

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