Cytogenetic Studies on Blood Lymphocytes From Patients With Schistosoma Mansoni

Shubber, E. K.; Salih, Hashim

doi:10.7883/yoken1952.40.137

Cited by 5 publications

(4 citation statements)

References 7 publications

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“…This work highlights the importance of evaluating the probiotics as a supplement for regular antiparasitic treatments (Mohamed et al, 2016). As other authors have demonstrated, infection by S. mansoni induces chromosomal aberrations and DNA damage (Shubber and Salih 1987) as well as praziquantel treatment (Montero and Ostrosky 1997) so in order to prevent this effect, El-Esawy et al used B. coagulans as a complement of anti-parasitic treatment. When anthelmintic treatment was combined with B. coagulans a significant reduction in chromosomal aberrations induced by infection or praziquantel treatment was observed.…”

Section: Trematodesmentioning

confidence: 81%

Searching for the one(s): Using Probiotics as Anthelmintic Treatments

Saracino¹,

Vila²,

Baldi³

et al. 2021

Front. Pharmacol.

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Helminths are a major health concern as over one billion people are infected worldwide and, despite the multiple efforts made, there is still no effective human vaccine against them. The most important drugs used nowadays to control helminth infections belong to the benzimidazoles, imidazothiazoles (levamisole) and macrocyclic lactones (avermectins and milbemycins) families. However, in the last 20 years, many publications have revealed increasing anthelmintic resistance in livestock which is both an economical and a potential health problem, even though very few have reported similar findings in human populations. To deal with this worrying limitation of anthelmintic drugs, alternative treatments based on plant extracts or probiotics have been developed. Probiotics are defined by the Food and Agriculture Organization as live microorganisms, which, when consumed in adequate amounts, confer a health benefit to the host. It has been proven that probiotic microbes have the ability to exert an immunomodulatory effect both at the mucosa and the systemic level. The immune response against gastrointestinal helminths is characterized as a type 2 response, with high IgE levels, increased numbers and/or activity of Th2 cells, type 2 innate lymphoid cells, eosinophils, basophils, mast cells, and alternatively activated macrophages. The oral administration of probiotics may contribute to controlling gastrointestinal helminth infections since it has been demonstrated that these microorganisms stimulate dendritic cells to elicit a type 2 or regulatory immune response, among other effects on the host immune system. Here we review the current knowledge about the use of probiotic bacteria as anthelmintic therapy or as a complement to traditional anthelmintic treatments. Considering all research papers reviewed, we may conclude that the effect generated by probiotics on helminth infection depends not only on the parasite species, their stage and localization but also on the administration scheme.

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Section: Trematodesmentioning

confidence: 81%

Searching for the one(s): Using Probiotics as Anthelmintic Treatments

Saracino¹,

Vila²,

Baldi³

et al. 2021

Front. Pharmacol.

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“…There are limited data available describing genotoxic properties of substances coming from helminths (Shubber and Salin 1987;Bekish and Bekish 2000). Mutagenic activity of different antigens from Ascaris suum has been observed by Bekish (1999Bekish ( , 2000Bekish ( , 2001.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic study of Ascaris trypsin inhibitor in cultured human lymphocytes with metabolic activation

Błaszkowska

Bratkowska

Łopaczyńska

et al. 2009

J Genet

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The trypsin inhibitor (ATI) isolated from gastrointestinal nematode Ascaris suum was tested in vitro for induction of chromosome aberrations and sister chromatid exchanges (SCE). Genotoxicity assessment of purified ATI was carried out on metaphase plates received from peripheral blood lymphocyte macroculture (48 h test of structural chromosome aberrations and 72 h test of SCE) with exogenous metabolic activation. ATI was tested in dose of 25, 50 and 100 microg per ml of culture. Kinetics of cell divisions were determined by the replication index (RI). The mitotic index (MI) was expressed as a number of metaphases per 1000 nuclei analysed. Analysis of chromosome aberrations showed that higher doses of ATI (50 and 100 microg/ml) significantly increased the frequency of chromosome aberrations (mainly of chromatid gaps and breaks) compared to the negative control. All concentrations of ATI caused a statistically significant reduction in the MI and RI. In comparison with the negative control, a significant increase in the SCE frequency was observed in all applied doses of ATI. Thus, in the presence of S9 activation, the Ascaris trypsin inhibitor showed potential clastogenic activity and inhibition of the dynamics of lymphocyte divisions.

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“…Such difference can be a result of small inversions, which may be caused by the influence of trematode infection. Thus, the trematodes can exert a mutagenic effect on their host (Shubber & Salih, 1987). But the action of microphallids on L. saxatilis can be more complicated.…”

Section: Discussionmentioning

confidence: 99%

On the karyology of trematodes of the genus Microphallus and their intermediate gastropod host, Littorina saxatilis II. Karyological study of Littorina saxatilis (Gastropoda: Prosobranchia)

Birstein

Mikhailova

1990

Genetica

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The karyotypes ofLittorina saxatilis Olivi specimens from exposed areas of the Yarnyshnaya inlet littoral (the Kola Peninsular, USSR) consist of 34 chromosomes, i.e. 10 pairs of meta-and submetacentrics and 7 pairs of subtelo-and telocentrics. Two pairs of bi-armed homologues are much bigger than the others. On the whole these karyotypes are similar to those of the individuals from the Swedish and English populations.

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Cytogenetic Studies on Blood Lymphocytes From Patients With Schistosoma Mansoni

Cited by 5 publications

References 7 publications

Searching for the one(s): Using Probiotics as Anthelmintic Treatments

Searching for the one(s): Using Probiotics as Anthelmintic Treatments

Cytogenetic study of Ascaris trypsin inhibitor in cultured human lymphocytes with metabolic activation

On the karyology of trematodes of the genus Microphallus and their intermediate gastropod host, Littorina saxatilis II. Karyological study of Littorina saxatilis (Gastropoda: Prosobranchia)

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