Cytogenetic studies on six species of the leuciscine genusPseudophoxinusBleeker, 1860 (Teleostei, Cyprinidae)

Abstract

Giemsa staining, C-and AgNOR-banding procedures. Diploid chromosome numbers of analyzed species were found to be the same (2n = 50) but their karyotypes formulae were different. For all species examined, the largest chromosome pair of the complements was subtelo-acrocentric. Heteromorphic sex chromosomes were not detected in any of the studied species. C-bands were found on the centromeres of several chromosomes in all studied species. NORs were detected in one pair of submetacentric chromosomes in P. burduricus, P. egridiri and P. fahrettini, and in two pairs of submetacentric chromosomes in P. battalgilae, P. evliyae and P. maeandri. Further, NOR polymorphisms were observed in some specimens of P. battalgilae, P. burduricus, P. evliyae and P. fahrettini for number, location and size. This study may contribute to other leuciscine cytogenetic studies.

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“…Frequently, this Ag-NORs are in the third sm chromosome in P. battalgilae and P. burduricus whereas in the fifth sm chromosome in P. egridiri and P. evliyae. This obtained results are in concordance with Ayata et al (2016) about midlle sized sm chromosomes with Ag-NORs. Otherwise, Ag-NOR locations in the karyotypes of P. battalgilae, P. burduricus, P. egridiri, P. evliyae and P. fahrettini are different from Anatolian leuciscines like P. antalyae which has the Ag-NORs in the second st chromosome (Ergene et al, 2010) and Chondrostoma beysehirense which has the Ag-NORs in the first sm/st chromosome pair (Arslan & Gündoğdu, 2016).…”

“…Ag-staining technique of Howell & Black (1980) was applied to chromosome preparations. Details about the modifications of the above mentioned techniques can be seen in Ayata et al (2016). Ag-stained preparations were scanned in the microscope and photographs of Ag-NOR metaphases were taken.…”

“…The number and locations of Ag-NORs have been used as a systematic and taxonomic character and these characters' benefit to fish cytotaxonomy (Boron, 1999). Ayata et al (2016) reported the single Ag-NORs in P. burduricus, P. egridiri and P. fahrettini whereas multiple Ag-NORs in P. battalgilae and P. evliyae. Additionally, they were pointed out that Ag-NOR bearing chromosomes varies from one to five in P. battalgilae; one to four in P. burduricus and P. fahrettini; two to six in P. evliyae.…”

“…Chromosomal studies in this genus have been started recently (Karasu, 2009). On the last decade, 12 species in total (Pseudophoxinus antalyae, P. battalgilae, P. burduricus, P. crassus, P. egridiri, P. elizavetae, P. evliyae, P. fahrettini, P. firati, P. hittitorum, P. maeandri and P. zekayi) have been studied karyologically (Ergene, Karahan, & Kuru, 2010;Karasu, Yüksel, & Gaffaroğlu, 2011;Gaffaroğlu, Karasu-Ayata, Ünal, & Yüksel, 2014;Unal, Gaffaroğlu, Ayata, & Yüksel, 2014;Ayata, Yüksel, & Gaffaroğlu, 2016;Ünal & Gaffaroğlu, 2016). In these studies, the diploid chromosome numbers (2n), fundamental arm numbers (FNs), chromosome morphologies and chromosome banding properties (with C-banding and silver-nucleolus organizer region (Ag-NOR) staining) have been revealed out.…”

Chromosomal Studies of <i>Luciobarbus kottelati</i> (Teleostei, Cyprinidae)

“…But the number of metacentric chromosomes show differences between them. This situation may be the result of pericentromeric inversions (and/or translocations involving centromeres) as reported by Ayata et al (2016). According to the chromosome number (2n=100), L. kottelati is tetraploids as reported for L. pectoralis (Unal and Gaffaroğlu 2016) and for several cyprinine barbines (Rab and Collares-Pereira 1995).…”