Cytogenetic Study of Aegopodium podagraria (Umbelliferae) for Use in Breeding

Romanov, Dmitry; Shirnin, S.Y.; Карлов, Г. И.; Divashuk, Mikhail G.

doi:10.3103/s0096392520020066

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2022

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“…C. sativa seeds were germinated on moist filter paper in Petri dishes at 24 °C in the dark for 72 h. The seedlings (2–3 cm long) were used to prepare the slides of mitotic metaphase chromosomes as described by Romanov et al [ 58 ].…”

Section: Methodsmentioning

confidence: 99%

Developing Oligo Probes for Chromosomes Identification in Hemp (Cannabis sativa L.)

Romanov

Карлов

Divashuk

2022

Plants

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Hemp (Cannabis sativa L., 2n = 20) is a valuable crop that is successfully used as a food, technical and medicinal crop. It is a dioecious plant with an XX\XY sex determination system. Some chromosomes of C. sativa have almost the same lengths and centromeric indexes. Cytogenetic markers help to distinguish similar plant chromosomes, including sex chromosomes, which is important for the breeding process. Two repeats (CS-1 and CS-237) were used to develop labeled oligo-probes for rapid and low-cost oligo-FISH. These oligos can be recommended for use as cytological markers to distinguish sex chromosomes (X and Y) and somatic chromosome pairs 3, 6, and 8 by rapid oligo-FISH in a short time.

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Section: Methodsmentioning

confidence: 99%