Cytogenetic Study of Northeastern Butterfly Lizard, <i>Leiolepis reevesii rubritaeniata</i> (Squamata, Agamidae) in Northeast Thailand

Abstract

SummaryThe present study aims to construct the karyotype and idiogram of the Northeastern butterfly lizard (Leiolepis reevesii rubritaeniata). Specimens were collected from Khon Kaen Province, Northeast Thailand. Lizard chromosome preparation was conducted by the squash technique using bone marrow and testis. Conventional staining and NOR-banding techniques were applied to stain the chromosomes with Giemsa s solution. The results showed that the number of diploid chromosome is 2n=36, while the fundamental number (NF) is 24 in both males and females. The types of macrochromosomes were six large metacentric, four medium metacentric, two small metacentric, and 24 microchromosomes. Nucleolar organizer regions (NORs) are located at the secondary constriction of long arm chromosomes, near the telomeres of the largest metacentric chromosomes. The karyotypes are not different for the sex chromosomes of both males and females. The karyotype formula is as follows:

Cross-Species BAC Mapping Highlights Conservation of Chromosome Synteny across Dragon Lizards (Squamata: Agamidae)

“…Sex determination mode is relatively well studied in a few species from the subfamily Amphibolurinae [7,19], but not in the other five subfamilies (Figure 1), highlighting a significant gap in our understanding of how sex chromosomes evolved in this widespread and chromosomally variable family. [6,17,20,21,24,31,[34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49]. The species of the subfamily Draconinae are distributed over South and Southeast Asia, Agaminae across Africa and Asia, Amphibolurinae across Australia, Papua New Guinea and Southeast Asia, Hydrosaurinae across Papua New Guinea, the Philippines and Indonesia, Leiolepidinae across Southeast Asia and Uromastycinae across Africa and South Asia.…”