Cytogenetical damage in exfoliated oral mucosa cells in elderly people suffering denture stomatitis

Matsumoto, Mariza Akemi; Castanho, Juliane; Kawakami, Roberto Yoshio; Ribeiro, Daniel Araki

doi:10.1111/j.1741-2358.2009.00315.x

Cited by 6 publications

(5 citation statements)

References 33 publications

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“…A further study not included in the table, carried out only in a group of patients with white oral lesions reports increased frequencies of MN and nuclear anomalies, mainly binucleated cells and fragmented nuclei, in lesion areas with respect to normal mucosa(18).Even for non-malignant diseases of the mouth with the exception of the study of Matsumoto,(74) significant differences in MN frequency between cases and controls were observed.A strong relationship was established between the MN frequency in patients with oral and head-Table 3describes a number of studies carried out in patients with tumors other than head-and-neck cancer. Three studies concerned breast cancer patients and all of them report a statistical significant increase of MN or MNC frequency in patients or their relatives compared with control groups(23,28,30).…”

mentioning

confidence: 93%

Clinical application of micronucleus test in exfoliated buccal cells: A systematic review and metanalysis

Bolognesi

Bonassi

Knasmueller

et al. 2015

Mutation Research/Reviews in Mutation Research

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mentioning

confidence: 93%

Clinical application of micronucleus test in exfoliated buccal cells: A systematic review and metanalysis

Bolognesi

Bonassi

Knasmueller

et al. 2015

Mutation Research/Reviews in Mutation Research

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“…Informed consent was designed according to the study, and approval was obtained from the ethical committee. The study considered those above 60 years of age as a geriatric category according to World Health Organization guidelines [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

Cytomorphometric Analysis of Buccal Exfoliated Cells in Geriatric and Pediatric Age Groups - A Cross-Sectional Study

Ramani,

Ramalingam

2023

Cureus

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Background We have to establish variations in cellular dimensions in buccal smears with respect to age. It can be used as a reference standard while dealing with age-related pathological abnormalities. Aim The study aims to compare the nuclear area (NA), cellular area (CA), and nucleus-to-cytoplasm ratio (N:C) of pediatric and geriatric age groups in smears obtained from clinically normal buccal mucosa. Materials and methods Buccal smears were collected from 60 subjects with age groups of <10 and >60 years. Cytological smears were prepared and fixed using alcohol. H&E and Papanicolaou's staining was performed as per manufacturer instructions. Cytomorphometric analysis was carried out using Image J software v.1.52 for CA, NA, and N:C. Statistical analysis using Student's t-test was performed using SPSS version 23.0 (IBM Inc, Armonk, New York). Results A significant difference (p<0.001) in NA and CA between the pediatric and geriatric age groups was noted. There was no significant difference in N:C among the study groups. Conclusion The present study provides baseline data of two different age groups that can be used for comparison of abnormal cells in suspicious clinical lesions.

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“…Epithelial cells are the first line of defense for the oral mucosa and thus may be the first to show genotoxic damage [2]. The micronucleus test (MN) using cells exfoliated from the oral epithelium is a method widely used to assess damage to deoxyribonucleic acid (DNA), genotoxicity and genomic instability, and various nuclear abnormalities serve as biomarkers of alterations in cytokinesis or cell death, facilitating assessment of the biocompatibility of specific materials and any pathologic conditions that may arise [3][4][5]. It has recently been recognized that metals used in orthodontic appliances may exert toxic effects on cells through direct and prolonged contact with oral tissues and metal corrosion [6].…”

Section: Introductionmentioning

confidence: 99%

Use of liquid-based cytology samples reveals genomic instability and cell death in patients undergoing orthodontic treatment

Carrillo-Novia¹,

Lara-Carrillo²,

Torres-Bugarı́n

et al. 2023

J Oral Sci

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Purpose: To examine the use of liquid-based exfoliative cytology to determine the presence of genomic instability and cell death in the oral mucosa of patients with orthodontic appliances. Methods: Fifty-four oral mucosa samples were collected from 18 patients and divided into three stages: T0, before fixation of orthodontic appliances; T1, 25 days after appliance fixation; T2, 90 days after appliance fixation. All samples were Papanicolaou-stained and observed by microscopy (1,000 cells/sample) to ascertain the frequency of micronucleated cells (MN) and nuclear abnormalities (nuclear buds (NB), binucleated (BN), condensed chromatin (CC), karyorrhexis (KR), and karyolysis (KL)). Differences were analyzed statistically using the Mann-Whitney, Wilcoxon, Kruskal-Wallis and chi-squared tests. Results: After placement of orthodontic appliances, significant differences were observed for genomic instability biomarkers (MN and NB) and cell death (CC, KR and KL) (P < 0.05). Female patients and older patients exhibited a higher frequency of MN. Conclusion: Liquid-based cytology has revealed that orthodontic appliances induce genomic instability and cell death in epithelial tissue of the oral mucosa, facilitating sample preservation and yielding more than one preparation per sample. Future studies should investigate whether such cell damage can be reversed through cell repair or whether cell alterations evolve and lead to disease.

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Cytogenetical damage in exfoliated oral mucosa cells in elderly people suffering denture stomatitis

Abstract: In summary, these data indicated that chronic denture stomatitis was able to induce cytotoxic effects as assessed by a micronucleus test.

Cited by 6 publications

References 33 publications

Clinical application of micronucleus test in exfoliated buccal cells: A systematic review and metanalysis

Clinical application of micronucleus test in exfoliated buccal cells: A systematic review and metanalysis

Cytomorphometric Analysis of Buccal Exfoliated Cells in Geriatric and Pediatric Age Groups - A Cross-Sectional Study

Use of liquid-based cytology samples reveals genomic instability and cell death in patients undergoing orthodontic treatment

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