Karyotype variability in successive generations after hybridization between the great sturgeon, Huso huso (L.), and the sterlet, Acipenser ruthenus L.The karyotypes ofbester (the hybrid between great sturgeon and sterlet) in threegenerationsdo not differ in diploid chromosome number from parental species and are displaced towards the sterlet in numbers of bi-and uni-armed chromosomes and chromosome arms. The variability of quantitative karyotypic parameters other than number of bi-armed chromosomes increases considerably (double, 0naverage)in F, and F2anddeclines to thelevelcharacteristicoftheparentalspeciesinF,.The variability results mainly from aneuploidy in microchromosomes and the karyotypic variabilities of 3 to 6-week-old and 4 to 5-month-old fish are the same. The stabilization of the hybrid karyotype in the third generation is rather rapid and may reflect the formation of a breed. The degree of individual karyotypic variability estimated on the basis of the coefficient of variation is 2-3% for parameters other than the number ofmicrochromosomes, for which it is 6 7 % .