Cytograding of mast cell tumours in dogs: destaining or not and staining with what?

Marcos, Ricardo; Macieira, Paula Cristina de Jesus Vilaça; Santos, Marta

doi:10.1111/vco.12290

Cited by 2 publications

(7 citation statements)

References 5 publications

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“…Notably, in the present study, the hypogranularity of RS does not appear to have influenced the determination of grading, probably due to the close relationship between hypogranularity and other cytologic criteria of malignancy. In turn, even the presence of massive granularity may limit the identification of high‐grade tumours, by hindering the recognition of malignant nuclear features (eg, mitosis, multinucleation, anisokaryosis), as recently suggested …”

Section: Discussionmentioning

confidence: 99%

“…recently suggested. 23,24 The diagnostic limits of RS became more evident in staging, where 3 independent observers failed in the identification of MCT metastases in 7% to 18% of RS-stained smears. Diagnostic problems were limited to the smears with low numbers of infiltrating mast cells, whereas in cases with a larger amount of mast cells, hypogranularity did not impair their recognition.…”

Section: Discussionmentioning

confidence: 99%

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Comparison between May‐Grünwald‐Giemsa and rapid cytological stains in fine‐needle aspirates of canine mast cell tumour: Diagnostic and prognostic implications

Sabattini

Renzi

Marconato

et al. 2018

Vet Comparative Oncology

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Mast cell tumours (MCTs) are often diagnosed by cytology based on the identification of purple intracytoplasmic granules with methanolic Romanowsky stains, including May-Grünwald-Giemsa (MGG). In clinical practice, aqueous rapid stains (RS) are commonly used, but mast cell granules may not stain properly. Aim of this prospective study was to investigate the frequency of MCT hypogranularity with RS and its potential implications in tumour identification, cytological grading assessment and recognition of nodal metastatic disease. Cytological preparations of canine primary MCTs and metastatic lymph nodes with subsequent histopathological confirmation were included. For each case, good-quality smears were stained with both MGG and RS and comparatively assessed. Eleven of 60 (18.3%) primary MCTs were hypogranular with RS; 9 of them were histologically high-grade tumours and in 3 cases (5%) a definitive MCT diagnosis could not be made. Accuracy in cytological grading assessment (85%) did not differ between RS and MGG. Thirteen of 28 (46.4%) metastatic lymph nodes were hypogranular with RS and 3 independent observers failed to identify nodal MCT metastases in 7% to 18% of RS-stained smears. This study confirms that, in limited cases, RS can be ineffective in staining MCT granules, particularly in high-grade tumours, thus making diagnosis more dependent on experience and quality of preparations. In dubious cases, methanolic stains should be applied. The use of RS is discouraged for the search of nodal metastases, as the identification of isolated mast cells can be more challenging.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparison between May‐Grünwald‐Giemsa and rapid cytological stains in fine‐needle aspirates of canine mast cell tumour: Diagnostic and prognostic implications

Sabattini

Renzi

Marconato

et al. 2018

Vet Comparative Oncology

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“…6,12,17 Moreover, nuclear features are often difficult to assess in routine cytologic preparations, especially in highly granulated ccMCTs. 9…”

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confidence: 99%

“…Coverslips were removed by immersion in xylene for 1–4 d, and slides were rehydrated and destained in an alcohol–acid solution, controlled by microscopic observation. After rinsing in tap water, slides were immersed in Gill hematoxylin overnight, 9 rinsed again in tap water, and stained with eosin (5 min).…”

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confidence: 99%

“…We cannot ignore that the destaining/restaining procedure may have changed the NA, although this seems unlikely from previous reports, in which direct comparisons between Diff-Quik and H&E smears were performed. 9 The availability of stereologic software may be another limitation to conducting stereology. This may be overcome by using manual methods or free software.…”

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confidence: 99%

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Canine mast cell tumors: utility of stereologic tools in cytology

et al. 2021

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Quantitative morphologic parameters assessed in cytologic samples of canine cutaneous mast cell tumors (ccMCTs) may assist with surgical planning and prognostication. Robust cutoffs can be defined, with high reproducibility, for parameters such as the nuclear area (NA). The NA may be determined by morphometry (image analysis, NAI) or by stereology, such as the 2D-nucleator method (NAN); stereologic techniques have not been applied to cytologic specimens of ccMCT, to our knowledge. We retrospectively selected routine cytology smears from 51 ccMCT cases and screened them to determine the percentage of neoplastic mast cells with indistinct nuclear borders; this was repeated after the slides were restained with H&E. The NAI and the NAN were estimated in 100 mast cells per animal in H&E-stained slides. All nuclei were visible in H&E smears, and unbiased quantification was feasible. The NAN was similar to NAI, but less time-consuming. Both the NAN and NAI determined by cytology differed in histologic low- and high-grade ccMCTs, and in histologic grade I plus II versus grade III ccMCTs. Stereologic parameters such as the NAN could be considered as complementary techniques for the cytologic evaluation of ccMCTs.

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Cytograding of mast cell tumours in dogs: destaining or not and staining with what?

Cited by 2 publications

References 5 publications

Comparison between May‐Grünwald‐Giemsa and rapid cytological stains in fine‐needle aspirates of canine mast cell tumour: Diagnostic and prognostic implications

Comparison between May‐Grünwald‐Giemsa and rapid cytological stains in fine‐needle aspirates of canine mast cell tumour: Diagnostic and prognostic implications

Canine mast cell tumors: utility of stereologic tools in cytology

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