Cytokeratin dynamics during oocyte maturation in the hamster requires reaching of metaphase I

Plancha, Carlos E.

doi:10.1046/j.1432-0436.1996.6020087.x

Cited by 11 publications

(14 citation statements)

References 67 publications

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“…Compared with microfilaments and microtubules, the third component of the cytoskeleton, the intermediate filaments, is still poorly understood. Plancha (1996) first described the organization and dynamics of cytoplasmic intermediate filaments during oocyte maturation in hamster. In prophase-I-arrested fully grown hamster oocytes, cytokeratins, the first cytoplasmic intermediate filament protein (Plancha et al, 1989), is confined to 4-10 large cortical aggregates, corresponding to extensive meshworks of intermediate filaments ( Figures.…”

Section: Reorganization Of the Cytoskeleton Redistributionmentioning

confidence: 99%

Behaviour of cytoplasmic organelles and cytoskeleton during oocyte maturation

Mao

Lou

et al. 2014

Reproductive BioMedicine Online

122

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Assisted reproduction technology (ART) has become an attractive option for infertility treatment and holds tremendous promise. However, at present, there is still room for improvement in its success rates. Oocyte maturation is a process by which the oocyte becomes competent for fertilization and subsequent embryo development. To better understand the mechanism underlying oocyte maturation and for the future improvement of assisted reproduction technology, this review focuses on the complex processes of cytoplasmic organelles and the dynamic alterations of the cytoskeleton that occur during oocyte maturation. Ovarian stimulation and in-vitro maturation are the major techniques used in assisted reproduction technology and their influence on the organelles of oocytes is also discussed. Since the first birth by assisted reproduction treatment was achieved in 1978, numerous techniques involved in assisted reproduction have been developed and have become attractive options for infertility treatment. However, the unsatisfactory success rate remains as a main challenge. Oocyte maturation is a process by which the oocyte becomes competent for fertilization and subsequent embryo development. Oocyte maturation includes both nuclear and cytoplasmic maturation. Nuclear maturation primarily involves chromosomal segregation, which has been well studied, whereas cytoplasmic maturation involves a series of complicated processes, and there are still many parts of this process that remain controversial. Ovarian stimulation and in-vitro maturation (IVM) are the major techniques of assisted reproduction. The effect of ovarian stimulation or IVM on the behaviour of cell organelles of the oocyte has been postulated as the reason for the reduced developmental potential of in-vitro-produced embryos. To further understanding of the mechanism of oocyte maturation and future improvement of assisted reproduction treatment, the complex events of cytoplasmic organelles and the cytoskeleton that occur during oocyte maturation and the influence of ovarian stimulation and IVM on these organelles are described in this review.

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Section: Reorganization Of the Cytoskeleton Redistributionmentioning

confidence: 99%

Behaviour of cytoplasmic organelles and cytoskeleton during oocyte maturation

Mao

Lou

et al. 2014

Reproductive BioMedicine Online

122

View full text Add to dashboard Cite

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“…The spindle is a unique subcellular structure that appears during the oocyte metaphase II (MII) stage, when the chromosomes are arranged on the compact metaphase plate located at the center of the spindle [12] . It is sensitive to many environmental factors, such as temperature and culture conditions [13,14] .…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Changes and Methylation of Human Oocytes Vitrified at the Germinal Vesicle Stage and Matured in vitro after Thawing

Liu

Zhou

Chu

et al. 2016

Gynecol Obstet Invest

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Aim: The study aimed to assess the effect of in vitro vitrification and maturation on the nuclear configuration, cytoplasmic maturation and global DNA methylation pattern of human germinal vesicle (GV) stage oocytes. Methods: Human oocytes from infertile women were randomly assigned to one of 3 groups: (i) metaphase II (MII) oocytes matured in vivo (vivo-MII, n = 56) as controls; (ii) MII oocytes matured in vitro (vitro-MII, n = 106); and (iii) MII oocytes that were vitrified at the GV stage, warmed and matured in vitro (cryo-MII, n = 122). All MII oocytes were fixed and immunofluorescence staining for spindle, chromosome, mitochondrion and cortical granules (CGs) were performed; examination was done using immunofluorescence laser scanning confocal microscope. The expression of 5-methycytosine in these MII oocytes was also assessed. Results: No significant difference was observed between vitro-MII and cryo-MII groups with respect to oocyte maturation rate (72.4 vs. 78.3%). No significant differences were observed in the distribution of mitochondria, migration of CG and global DNA methylation pattern among the 3 study groups. However, the abnormal configuration of spindle and chromosome was significantly higher in cryo-MII group (78.9 and 84.2%) as compared to that in the vitro-MII (45.0 and 50.0%, p < 0.05) and vivo-MII groups (27.3 and 36.4%, p < 0.05). Conclusion: GV oocytes appeared to resist vitrification and retain their potential for maturation to MII stage oocytes after thawing. However, GV oocytes vitrification combined with in vitro maturation could affect the organization of spindle and chromosome.

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“…Subsequently, the activity increases and is required for post‐GVBD events (Fan and Sun 2004). Reportedly, microfilaments and microtubules are not required for keratin reorganization during hamster oocyte maturation (Plancha 1996). Therefore, our results suggest that MAPKK might participate in the signal network regulating distribution of keratin, probably by phosphorylating keratin and/or keratin‐associated proteins after GVBD.…”

Section: Discussionmentioning

confidence: 99%

“…1983), mice (Chisholm and Houliston 1987), hamsters (Plancha et al. 1989; Plancha 1996), goats (Gall et al. 1989) and humans (Santini et al.…”

Section: Introductionmentioning

confidence: 99%

“…The expression pattern of intermediate filaments is cell-specific and tissue-specific (Hyder et al 2008). Although keratin is the major structural protein in epithelial cells (Kirfel et al 2003), previous reports show that keratin is also present in the oocytes and early embryos in Xenopus (Franz et al 1983), mice (Chisholm and Houliston 1987), hamsters (Plancha et al 1989;Plancha 1996), goats (Gall et al 1989) and humans (Santini et al 1993). However, the physiological significance of keratin reorganization and its regulating mechanism remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

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Intermediate Filament Keratin Dynamics During Oocyte Maturation Requires Maturation/M-Phase Promoting Factor and Mitogen-Activated Protein Kinase Kinase Activities in the Hamster

Kabashima

Matsuzaki

Suzuki

2009

Reproduction in Domestic Animals

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Research related to intermediate filaments in mammalian oocytes remains poorly advanced. We investigated keratin reorganization in oocytes during meiotic maturation using immunofluorescence, and examined effects of inhibitors for cdc2 and mitogen-activated protein kinase kinase (MAPKK) on keratin assembly. In germinal vesicle (GV) oocytes (n = 26), large and oval-shaped aggregates of non-fibrillar keratin were found in the cortical ooplasm (designated as a 'cortical' pattern). The delicate network of keratin filaments was concentrated in the GV periphery. The large keratin aggregates began to divide into small fragments at the pro-MI/MI stage (n = 22, designated as a 'fragmented' pattern). Some keratin fragments were occasionally broken down into several granules at the peripheral region. In the MII oocytes (n = 24), the filament network was extended over the ooplasm and numerous keratin granules were scattered across the oocyte (designated as a 'granular' pattern). After 12 h of incubation with roscovitine, 66.7% of the oocytes (20/30) were at the GV stage and showed a cortical pattern of keratin. After incubation with U0126, most oocytes (83.9%, 26/31) were at the MII stage; most of them (76.9%, 20/26) showed a fragmented pattern of keratin. The increasing complexity of keratin filament network from the GV to MII stages suggests a possible role in maintaining cell integrity under physical stress after ovulation. In fact, maturation/M-phase promoting factor is necessary for such keratin reorganization, as is meiotic nuclear progression. In addition, MAPKK is involved in keratin reorganization from a fragmented pattern to a granular pattern.

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Cytokeratin dynamics during oocyte maturation in the hamster requires reaching of metaphase I

Cited by 11 publications

References 67 publications

Behaviour of cytoplasmic organelles and cytoskeleton during oocyte maturation

Behaviour of cytoplasmic organelles and cytoskeleton during oocyte maturation

Ultrastructural Changes and Methylation of Human Oocytes Vitrified at the Germinal Vesicle Stage and Matured in vitro after Thawing

Intermediate Filament Keratin Dynamics During Oocyte Maturation Requires Maturation/M-Phase Promoting Factor and Mitogen-Activated Protein Kinase Kinase Activities in the Hamster

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