Cytokeratin expression in epidermal stem cells in skin adnexal tumors

Abstract

The expression levels of seven types of cytokeratin (CK) in different kinds of skin adnexal tumors were evaluated. One hundred and thirty-two patients with different kinds of skin adnexal tumors admitted and treated in the Department of Dermatology of Dongying People's Hospital from May 2013 to May 2015 were selected and underwent tissue section staining. Another 20 cases of normal skin were enrolled as the control group. The expression levels of the seven types of CK in different kinds of skin appendages were observed and recorded. The expression levels of the seven types of CK in the 132 cases of skin adnexal tumor tissues were different. CK10 was mainly expressed in squamous cell carcinoma, but it was not expressed in basal cell carcinoma. CK19 was expressed in basal cell carcinoma, but its expression was not detected in squamous cell carcinoma. As the degree of differentiation was increased in the epidermis, hair follicle and sebaceous gland, the expressed molecular weight of CK was augmented gradually. The expression levels of five types of CK (namely, CK8, CK10, CK14, CK18 and CK19) could be measured in the squamous cell carcinoma and basal cell carcinoma. Statistical analysis revealed that there were statistically significant differences in the expression levels of these five types of CK in the two cell carcinomas (P<0.05). Five types of CK, i.e., CK7, CK8, CK17, CK18 and CK19, had markedly different expression levels in hair follicle tumor and sweat gland tumor, which were statistically significant (P<0.05). The expression levels of a group of CKs detected by virtue of semi-quantitative analysis via immunohistochemistry can be regarded as one of the important indexes for clinical diagnosis of skin adnexal tumors.

The Epithelial Cytokeratins 15 and 19 Gene Expression in Non-Segmental Vitiligo Patients under NB-UVB Phototherapy

“…Through measuring the level of cytokeratins 15 and 19 among the patients in comparison with control, we found that cytokeratins are expressed in [21] who studied these CKs expression in adnexal neoplasm in comparison with normal subjects. When CKs were measured in the patients after they received NB-UVB for 4 th months, they are found higher than control and this agrees with Goldstein et al [22] who reported the effect of NB-UVB on proliferation and differentiation of Melscs.…”

Adipose mesenchymal stem cells combined with platelet-rich plasma accelerate diabetic wound healing by modulating the Notch pathway

“…Concomitant with these results, Yang et al 2016 [ 39 ] reported that initial activation of Notch1 signaling throughout wound healing could support EPSC proliferation and preserve their low differentiation potential and even multi-directional differentiation potential. Furthermore, Yang et al 2016 [ 39 ] and Zhou et al 2018 [ 40 ] stated that Notch1 is extensively expressed on EPSCs which were traced mainly in the basal layer of the epidermis, so, if the Notch signaling pathway was blocked, EPSC proliferation would be inhibited hindering the epithelialization process leading to loss of skin barrier function.…”

Epidermal Stem Cells in Wound Healing and Regeneration

“…Keratin is an important structural protein of epidermal cells. The different degrees of differentiation of epidermal cells can be used to express different characteristics of keratin, which can be used for identifying EpiSCs, TACs, and TDCs [31]. EpiSCs express mainly keratin 15 and 19 (CK15 and CK19); TACs express keratin 5 and 14 (CK5 and CK14); and TDCs express keratin 1 and 10 (CK1 and CK10).…”