Cytokine adjuvancy of BVDV DNA vaccine enhances both humoral and cellular immune responses in mice

Nobiron, Isabelle; Thompson, Ian; Brownlie, Joe; Collins, Margaret E.

doi:10.1016/s0264-410x(01)00157-8

Cited by 52 publications

(27 citation statements)

References 40 publications

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“…18,26,61,119 The changes in cytokine production by immune or nonimmune cells affect both innate and adaptive immunity. 108 The persistently infected (PI) animal serves as the reservoir of BVDV and a source of infection. Recent advances allow better detection of PI animals and thus improve the possibility of enhanced control.…”

Section: Introductionmentioning

confidence: 99%

Bovine Viral Diarrhea Virus Infections

Brodersen

2014

Vet Pathol

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Bovine viral diarrhea virus (BVDV) continues to be of economic significance to the livestock industry in terms of acute disease and fetal loss. Many of the lesions relating to BVDV infection have been well described previously. The virus is perpetuated in herds through the presence of calves that are persistently infected. Relationships between various species and biotypes of BVDV and host defenses are increasingly understood. Understanding of the host defense mechanisms of innate immunity and adaptive immunity continues to improve, and the effects of the virus on these immune mechanisms are being used to explain how persistent infection develops. The noncytopathic biotype of BVDV plays the major role in its effects on the host defenses by inhibiting various aspects of the innate immune system and creation of immunotolerance in the fetus during early gestation. Recent advances have allowed for development of affordable test strategies to identify and remove persistently infected animals. With these improved tests and removal strategies, the livestock industry can begin more widespread effective control programs.

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Section: Introductionmentioning

confidence: 99%

Bovine Viral Diarrhea Virus Infections

Brodersen

2014

Vet Pathol

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“…Harpin et al [26,27] showed that 3 / 4 vaccinated mice cross-neutralised BVDV-1b strain New-York as well as BVDV-1a NADL but not BVDV-2 strain 125, indicating that cross-neutralisation is restricted to the genotype from which the coding sequence had been cloned. Another report by Nobiron et al [46] showed that the response to heterologous strain NADL was much lower in terms of titres and numbers of responding mice than to homologous strain Ky1203nc though both belong to the same genotype Ia. In cattle, the antibodies neutralised only homologous strain Ky1203nc but not NADL [47].…”

Section: Discussionmentioning

confidence: 95%

“…Indeed, whereas Harpin's construct encodes a full-length E2 including its predicted native cleavage site and hence native N-terminus, Nobiron's is deleted at the C-terminus and uses an immunoglobulin signal peptide with its own cleavage site thus creating a truncated E2 protein with a chimeric N-terminus (the first 17 residues of the mature protein are from the multiple cloning site of vector pSecTag). Although titres were higher with the truncated E2 construct, cross-neutralisation of strains within genotype Ia was absent or poor even when genetic adjuvant IL-2 and GM-CSF were co-inoculated [45,46]. Accordingly, cross-neutralisation of BVDV strains from the same viral species depended on the integral E2 protein which should include its native N-terminus.…”

Section: Discussionmentioning

confidence: 98%

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Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle

et al. 2007

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-We report DNA immunisation experiments in cattle using plasmid constructs that encoded glycoprotein E2 from bovine viral diarrhoea virus (BVDV)-1 (E2.1) and BVDV-2 (E2.2). The coding sequences were optimised for efficient expression in mammalian cells. A modified leader peptide sequence from protein gD of BoHV1 was inserted upstream of the E2 coding sequences for efficient membrane export of the proteins. Recombinant E2 were efficiently expressed in COS7 cells and they presented the native viral epitopes as judged by differential recognition by antisera from cattle infected with BVDV-1 or BVDV-2. Inoculation of pooled plasmid DNA in young cattle elicited antibodies capable of neutralising viral strains representing the major circulating BVDV genotypes.BVDV / DNA immunisation / E2 / neutralisation / synthetic gene

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“…with DNA vaccines expressing varicellazoster virus also produce IgG2a (Stasikova et al, 2003), and a DNA vaccine containing SARS coronavirus nucleocapsid (N) protein leads to increased levels of IgG2a in mice (Zhao et al, 2005). Although the immune response generated by a DNA vaccine can be influenced via co-delivery of adjuvant containing cytokine genes or by different routes of injection Feltquate et al, 1997;Gurunathan et al, 2000;He et al, 2005He et al, , 2006Hung et al, 2007;Kim et al, 2001;Nobiron et al, 2001;Osorio et al, 1999;Rogers et al, 1999;Saldarriaga et al, 2006;Song et al, 2000Song et al, , 2008Whitmire et al, 2005). In this study, we injected mice with only DNA plasmids by the i.m.…”

Section: Fig 4 Induction Of Cd4+ and Cd8+ T-cells By Dna Vaccinesmentioning

confidence: 99%

Mice immunogenicity after vaccination by DNA vaccines containing individual genes of a new type of reovirus

Bai¹,

Shen²,

Hu³

et al. 2013

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Summary. -In this study, we investigated humoral and cellular immune responses in mice to DNA vaccines containing individual S or M genes of a new type of reovirus (nRV) isolate from a severe acute respiratory syndrome (SARS) patient in Beijing, China. Mice were immunized intramuscularly (i.m.) with 100 μg of S1, S2, S3, S4, M1, M2, and M3 DNA vaccine each 4 times in 2-week intervals and assayed for humoral IgG, IgG1, IgG2, and IgG2b antibodies by ELISA and for cellular immune response, particularly IFN-γ induction by ELISpot assay. Moreover, CD4+ and CD8+ T cell levels in peripheral blood mononuclear cells were assayed by flow cytometry. We found that all DNA vaccines induced IgG antibodies, predominantly of the IgG2a class and S3 DNA vaccine was the strongest inducer. M2 and S3 DNA vaccines elicited Th1-and Th2-based immune responses, respectively, while S1 and M3 DNA vaccines induced a mixed Th1/Th2 response. M1, S2, and S4 DNA vaccines were poorly immunogenic. To our knowledge, this is the first report characterizing mammalian reovirus DNA vaccines applied to a mouse model. Keywords: reovirus; DNA vaccine; SARS; mouse; immunogenicity *Corresponding author. E-mail: maopy302@hotmail.com; phone: +86-10-66933316.# Bingke Bai and Honghui Shen contributed equally to this paper. Abbreviations: ELISpot = enzyme-linked immunosorbent spot assay; IFN-γ = interferon gamma; i.m. = intramuscularly; R4 = new type of reovirus; SARS = severe acute respiratory syndrome; SFC = spot-forming cells

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Cytokine adjuvancy of BVDV DNA vaccine enhances both humoral and cellular immune responses in mice

Cited by 52 publications

References 40 publications

Bovine Viral Diarrhea Virus Infections

Bovine Viral Diarrhea Virus Infections

Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle

Mice immunogenicity after vaccination by DNA vaccines containing individual genes of a new type of reovirus

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