Cytokine expression by CD163+ monocytes in healthy and Actinobacillus pleuropneumoniae-infected pigs

Jarosova, Rea; Ondráčková, Petra; Levá, Lenka; Nedbalcová, Kateřína; Vícenová, Monika; Mašek, Josef; Volf, Jiřı́; Gebauer, Jan; Do, Tomáš; Guráň, Roman; Sládek, Z.; Domı́nguez, Javier; Faldyna, Martin

doi:10.1016/j.rvsc.2022.07.015

Cited by 1 publication

(1 citation statement)

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“…As an innate immune sensor and inducer of local inflammation, porcine CD163 promotes inflammatory cytokine production induced by Gram-positive Actinobacillus pleuropneumoniae (APP) in monocytes/macrophages [ 31 ]. In addition, studies in humans support that cytokine production can be triggered by signals responding to bacterial recognition by CD163 [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Macrophage Scavenger Receptor CD163 in Mediating Glaesserella parasuis Infection of Host Cells

Deng

Zhu

et al. 2023

Veterinary Sciences

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The macrophage CD163 surface glycoprotein is a member of the SRCR family class B, which has been identified as the key trigger in host–pathogen interactions, but its specific roles in sensing Glaesserella parasuis (G. parasuis) infection are largely unknown. Here, we investigated porcine CD163 in mediating the adhesion and immune response of G. parasuis using in vitro host–bacteria interaction models. CD163-overexpressing Chinese hamster ovary K1 cells (CHO-K1) showed obvious subcellular localization in the cytoplasm, especially in the cytomembrane. Although detection using scanning electron microscopy (SEM) confirmed the bacterial adhesion, there was no significant difference in the adhesion of G. parasuis to CHO-K1 cells between the presence and absence of CD163. In addition, similar results were observed in 3D4/21 cells. Meanwhile, bindings of G. parasuis to nine synthetic peptides, the bacterial binding motifs within SRCR domains of CD163, were weak based on a solid-phase adhesion assay and agglutination assay. Moreover, CD163 had no effect on the expression of G. parasuis-induced inflammatory cytokines (IL-6, INF-γ, IL-10, IL-4 and TGF-β) in CHO-K1 cells. In conclusion, these findings indicate that porcine CD163 plays a minor role in sensing G. parasuis infection.

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Section: Discussionmentioning

confidence: 99%