Cytokine gene polymorphisms implicated in the pathogenesis of Plasmodium falciparum infection outcome

Segbefia, Selorm Philip; Asandem, Diana Asema; Amoah, Linda Eva; Kusi, Kwadwo Asamoah

doi:10.3389/fimmu.2024.1285411

Cited by 2 publications

(1 citation statement)

References 132 publications

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“…This may be due to its pleiotropic role in the human body. 33 , 34 , 35 , 36 Also, the levels of other cytokines (IL‐1β, IL‐2, IL‐10, and TNF‐α) were also comparable between the two groups. This supports the findings of Ribeiro et al 37 where cytokine levels were not significantly different between CHB (without antiviral therapy) and healthy individuals.…”

Section: Discussionmentioning

confidence: 79%

Expression patterns of immune checkpoint proteins and Plasmodium falciparum‐induced cytokines in chronic hepatitis B virus‐infected and uninfected individuals: A cross‐sectional study

Segbefia,

Asandem,

Pobee

et al. 2024

Health Science Reports

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Background and AimChronic hepatitis B virus (CHB) infection remains a major public health problem. The American Association for the Study of Liver Diseases (AASLD) 2018 Hepatitis B Guidelines provide that CHB individuals not requiring antiviral therapy yet are monitored to determine the need for antiviral therapy in the future; however, these tests do not include measurement of cytokines and immune cell characterization. This case‐control study compared the cytokine and immune checkpoint protein expression profiles between CHB individuals not yet on antiviral treatment and hepatitis B virus (HBV)‐negative individuals.MethodsCD4 and CD8 T cells from CHB and HBV‐negative individuals were characterized for immune checkpoint proteins programmed cell death‐1 (PD1), T cell Immunoglobulin domain and mucin domain‐containing protein 3 (TIM‐3), and cytotoxic T lymphocyte‐associated antigen 4 (CTLA‐4) (CD152), and a memory marker CXCR3 (CD183) using flow cytometry. Malaria‐induced cytokine expression levels were determined by stimulating their blood cells with Plasmodium falciparum 3D7 strain antigens (CSP, AMA‐1, and TRAP) in whole blood assays, and cytokine levels were measured using a 13‐plex Luminex kit.ResultsHBV‐negative and CHB individuals had comparable levels of CD4+ and CD8+ T cells. However, a proportion of the CD4+ and CD8+ populations from both groups, which were CXCR3+, expressed PD‐1 and CD152. The ability to produce cytokines in response to malaria antigen stimulation was not significantly different between the groups.ConclusionThese findings support excluding CHB individuals from antiviral therapy at this stage of infection. However, CHB individuals require regular monitoring to determine the need for later antiviral treatment.

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Section: Discussionmentioning

confidence: 79%

Expression patterns of immune checkpoint proteins and Plasmodium falciparum‐induced cytokines in chronic hepatitis B virus‐infected and uninfected individuals: A cross‐sectional study

Segbefia,

Asandem,

Pobee

et al. 2024

Health Science Reports

View full text Add to dashboard Cite

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Interleukin-4 and its receptor alpha in paediatric uncomplicated malaria patients from a Ghanaian case-control study

Diaba-Nuhoho

2024

BMC Res Notes

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Objectives This study investigated gene polymorphisms in the interleukin-4 (IL-4) and its receptor alpha (IL-4Rα) gene regions in human hosts with uncomplicated malaria. Data description Blood samples were obtained from a case-control study conducted at the Sogakope district hospital in the Volta region of Ghana. Thick blood films were made and used to detect the presence and levels of parasitaemia in the patient samples. Genotyping of IL-4 (150 C/T) and the IL-4Rα (Pro-478-Ser) polymorphisms in the promoter regions and receptor gene was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) after DNA extraction. The data are useful in determining genetic polymorphisms (allele and genotypic frequencies) of IL-4 and its alpha receptor. In addition, they are useful when comparing levels of parasite density and haematological parameters between genotypic variants of IL-4 and IL-4Rα. These data contribute to our understanding of the genetic basis of malaria susceptibility, particularly in the population of the Volta region of Ghana.

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Cytokine gene polymorphisms implicated in the pathogenesis of Plasmodium falciparum infection outcome

Cited by 2 publications

References 132 publications

Expression patterns of immune checkpoint proteins and Plasmodium falciparum‐induced cytokines in chronic hepatitis B virus‐infected and uninfected individuals: A cross‐sectional study

Expression patterns of immune checkpoint proteins and Plasmodium falciparum‐induced cytokines in chronic hepatitis B virus‐infected and uninfected individuals: A cross‐sectional study

Interleukin-4 and its receptor alpha in paediatric uncomplicated malaria patients from a Ghanaian case-control study

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