Cytokine immunotrapping: an assay to study the kinetics of production and consumption or degradation of human interferon-γ

Akdiş, A.C.; Towbin, Harry; Libsig, Patrice; Motz, Jutta; Alkan, Şefik Ş.

doi:10.1016/0022-1759(95)00055-f

Cited by 16 publications

(7 citation statements)

References 25 publications

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“…The column was washed three times with 500 μl of cold MACS buffer then removed from the magnet and the CD4 + fraction eluted with 1 ml MACS buffer and firm pressure on the column from a plunger. CD4 + T cells isolated using this method of positive selection have been shown to maintain functional activity (Akdis et al, 1995). …”

Section: Methodsmentioning

confidence: 99%

CCR6+ Th cells in the cerebrospinal fluid of persons with multiple sclerosis are dominated by pathogenic non-classic Th1 cells and GM-CSF-only-secreting Th cells

Restorick

Durant

Kalra

et al. 2017

Brain, Behavior, and Immunity

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Section: Methodsmentioning

confidence: 99%

CCR6+ Th cells in the cerebrospinal fluid of persons with multiple sclerosis are dominated by pathogenic non-classic Th1 cells and GM-CSF-only-secreting Th cells

Restorick

Durant

Kalra

et al. 2017

Brain, Behavior, and Immunity

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“…IL-4 differentiates peripheral blood monocytes into dendritic cells [43] and induces apoptosis in monocytes [44]. Similarly, active consumption of IFN-y was observed by monocyte-containing cell cultures [45], and rapid elimination of IL-4 by monocytes may explain observations that IL-4 was more efficiently produced in monocyte-depleted lymphocyte cultures [17]. This suggests that inhibition of IL-4 consumption and enhancement of total IgE and IgG4 production by GC in PBMC are functionally related and depend on high IL-4 levels for isotype switch [%lo].…”

Section: Gc Inhibit Il-4 Consumption By Monocytes and Pbmcmentioning

confidence: 98%

Glucocorticoids inhibit human antigen‐specific and enhance total IgE and IgG4 production due to differential effects on T and B cells in vitro

Akdiş¹,

Blesken²,

Akdiş³

et al. 1997

Eur J Immunol

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Although anti-inflammatory properties of glucocorticoids (GC) are well documented, their activity in allergic diseases is still controversial. Recently, it has been reported that GC can increase, both in vivo and in vitro, the polyclonal production of total IgE. In this study we investigated the effects of GC on the antigen (Ag)-specific IgE response in a human in vitro system with peripheral blood mononuclear cells or B cells of bee venom-sensitized individuals that allows the production of bee venom phospholipase A2 (PLA)-specific IgE and IgG4 antibodies (Ab). PLA-specific Ab were induced by simultaneously activating T cells and B cells specifically with allergen and polyclonally with anti-CD2 and soluble CD40 ligand (sCD40L) in the presence of interleukin (IL)-4. Indeed, dexamethasone and prednisolone enhanced the formation of total IgE and IgG4 in PBMC, while the production of PLA-specific IgE and IgG4 Ab was selectively inhibited in a dose-dependent manner. The suppressive effect of GC was mediated during Ag-specific stimulation and T cell-B cell interaction. This was due to GC suppressing specific T cell proliferation and cytokine production, whereas neither allergen-specific nor total IgE and IgG4 production by sCD40L/IL-4-stimulated pure B cells was affected. In contrast to GC, cyclosporine A inhibited both total and PLA-specific IgE and IgG4 secretion in peripheral blood mononuclear cells and B cell cultures. Further experiments showed that increase in nonspecific total isotype response resulted from inhibition of IL-4 uptake by cells other than B cells and sufficient availability of IL-4 to B cells for isotype switch and synthesis. Furthermore, demonstration of opposite regulatory effects of GC on specific and total isotype formation in vitro, including the inhibition of allergy-relevant Ag-specific IgE response, may contribute to a better understanding of apparently controversial observations, and explain why most allergic patients benefit from GC therapy.

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“…LPS and/or PHA are usually used as stimuli for cytokine synthesis and particularly for proinflammatory cytokines (IL-1, IL-6, IL-8, TNFα) or Th1/Th2 cytokines (IFNγ, IL-10). Released cytokines are either measured in the cell supernatant by ELISA (19) or immunotrapped by specific antibodies coating culture wells or beads (20,21). These last modifications add sensitivity to the tests.…”

Section: Determination Of the Capacity Of Cells To Secrete Cytokinesmentioning

confidence: 99%

The Clinical Usefulness of the Measurement of Cytokines

Bienvenu

Monneret²,

Fabien³

et al. 2000

CCLM

102

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The utilization of accurate and sensitive methods for the measurement of cytokines in body fluids is prerequisite for the proper use of these mediators in clinical practice. Many factors contribute to the complexity of cytokine quantitation. Bioassays historically preceded immunoassays, which are now very popular, but there is a need for standardization. Nevertheless, due to the local effects of cytokines, the study of their blood levels is of limited value for an understanding of the pathophysiology of these mediators. This explains the development of alternative approaches to assess the ability of cells to produce cytokines. These include the Enzyme-Linked Immuno Spot Assay (ELISPOT), the measurement of cell-associated cytokines by flow cytometry, and the study of cytokine secretion by isolated peripheral blood mononuclear cells or by whole blood test. All these techniques, associated with a local detection of cytokines by immunohistochemistry or in situ hybridization and reverse transcriptase polymerase chain reaction, appear to be complementary tools for a better understanding of the biology of cytokines. Selected examples of possible clinical applications related to infectious diseases, cancer, autoimmune diseases, allergy, transplantation and preclinical evaluation of drugs and biotechnology products are given.

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Cytokine immunotrapping: an assay to study the kinetics of production and consumption or degradation of human interferon-γ

Cited by 16 publications

References 25 publications

CCR6+ Th cells in the cerebrospinal fluid of persons with multiple sclerosis are dominated by pathogenic non-classic Th1 cells and GM-CSF-only-secreting Th cells

CCR6+ Th cells in the cerebrospinal fluid of persons with multiple sclerosis are dominated by pathogenic non-classic Th1 cells and GM-CSF-only-secreting Th cells

Glucocorticoids inhibit human antigen‐specific and enhance total IgE and IgG4 production due to differential effects on T and B cells in vitro

The Clinical Usefulness of the Measurement of Cytokines

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