Cytokine-induced differentiation of multipotent adult progenitor cells into functional smooth muscle cells

Ross, Jeffrey; Hong, Zhigang; Willenbring, Ben; Zeng, Lepeng; Isenberg, Brett C.; Lee, Eu Han; Reyes, Morayma; Keirstead, Susan A.; Weir, E. K.; Tranquillo, Robert T.; Verfaillie, Catherine M.

doi:10.1172/jci28184

Cited by 164 publications

(113 citation statements)

References 56 publications

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“…When the P2 cells were cultured in RGM or RGM supplemented with PDGF-BB (50 ng/ml), a potent inducer for mesenchymal stem cell differentiation into SMCs (Ross et al 2006), we observed that PDGF significantly induced a robust upregulation of αSMA, SM22α, calponin and smoothelin, whereas RGM per se led to only a slight upregulation of αSMA and SM22α (Fig. 3b, c).…”

Section: Brasmcs^cultured In Scgm Have Multipotencymentioning

confidence: 86%

“…RASMCs maintained in SCGM were differentiated into SMCs by platelet-derived growth factor (PDGF) as previously reported (Ross et al 2006;Tang et al 2012). Briefly, the isolated RASMCs, which were cultured in SCGM for 14 days (P2), were switched to high-glucose DMEM containing 5 % FBS (Invitrogen) and PDGF-BB (50 ng/ml; cat: 315-18, Peprotech) for 7 days.…”

Section: Cell Differentiationmentioning

confidence: 99%

“…Multipotency of MVSCs vs. phenotypic modulation or plasticity of VSMCs PDGF-BB is well established as enabling the SMC differentiation of stem cells while inducing the dedifferentiation or synthetic phenotype of VSMCs (Ross et al 2006). Our results clearly show that PDGF-BB induces synthetic SMC differentiation of the undifferentiated P2 BRASMCs^, which have the potential for adipocyte and bone cell differentiation, while promoting the dedifferentiation of the differentiated P5-10 RASMCs, which lose their multipotency.…”

Section: Origins Of Mvscsmentioning

confidence: 99%

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Inhibitory role of reactive oxygen species in the differentiation of multipotent vascular stem cells into vascular smooth muscle cells in rats: a novel aspect of traditional culture of rat aortic smooth muscle cells

Song¹,

Wang²,

Wu³

et al. 2015

Cell Tissue Res

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Proliferative or synthetic vascular smooth muscle cells (VSMCs) are widely accepted to be mainly derived from the dedifferentiation or phenotypic modulation of mature contractile VSMCs, i.e., a phenotype switch from a normally quiescent and contractile type into a proliferative or synthetic form. However, this theory has been challenged by recent evidence that synthetic VSMCs predominantly originate instead from media-derived multipotent vascular stem cells (MVSCs). To test these hypotheses further, we re-examine whether the conventional rat aortic SMC (RASMC) culture involves the VSMC differentiation of MVSCs or the dedifferentiation of mature VSMCs and the potential mechanism for controlling the synthetic phenotype of RASMCs. We enzymatically isolated RASMCs and cultured the cells in both a regular growth medium (RGM) and a stem cell growth medium (SCGM). Regardless of culture conditions, only a small portion of freshly isolated RASMCs attaches, survives and grows slowly during the first 7 days of primary culture, while expressing both SMC- and MVSC-specific markers. RGM-cultured cells undergo a process of synthetic SMC differentiation, whereas SCGM-cultured cells can be differentiated into not only synthetic SMCs but also other somatic cells. Notably, compared with the RGM-cultured differentiated RASMCs, the SCGM-cultured undifferentiated cells exhibit the phenotype of MVSCs and generate greater amounts of reactive oxygen species (ROS) that act as a negative regulator of differentiation into synthetic VSMCs. Knockdown of phospholipase A2, group 7 (Pla2g7) suppresses ROS formation in the MVSCs while enhancing SMC differentiation of MVSCs. These results suggest that cultured synthetic VSMCs can be derived from the SMC differentiation of MVSCs with ROS as a negative regulator.

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Section: Brasmcs^cultured In Scgm Have Multipotencymentioning

confidence: 86%

Section: Cell Differentiationmentioning

confidence: 99%

Section: Origins Of Mvscsmentioning

confidence: 99%

See 1 more Smart Citation

Inhibitory role of reactive oxygen species in the differentiation of multipotent vascular stem cells into vascular smooth muscle cells in rats: a novel aspect of traditional culture of rat aortic smooth muscle cells

Song¹,

Wang²,

Wu³

et al. 2015

Cell Tissue Res

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“…SF conditions have been used in previous SMC differentiation experimentations [25] to avoid non-specific detection of some SMC differentiation markers. Differentiation was induced in SF-medium supplemented with growth factors (GF), i.e.…”

Section: Smooth Muscle Cell Differentiation Of Emscsmentioning

confidence: 99%

Induction of endometrial mesenchymal stem cells into tissue-forming cells suitable for fascial repair

Edwards

Tan³

et al. 2014

Acta Biomaterialia

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“…In vitro MAPCs can give rise to functional cell types from the three embryonic germ layers, including functional neuronal cells [168,179,181,228]. Zhao et al [232] have employed MAPCs in a rodent model of stroke, showing a behavioral function improvement, even if there is no evidence for their neural differentiation in vivo [25].…”

Section: Bone Marrow-derived Cellsmentioning

confidence: 99%

Recent therapeutic strategies for spinal cord injury treatment: possible role of stem cells

Garbossa¹,

Boido

Fontanella³

et al. 2012

Neurosurg Rev

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Spinal cord injury (SCI) often results in significant dysfunction and disability. A series of treatments have been proposed to prevent and overcome the formation of the glial scar and inhibitory factors to axon regrowth. In the last decade, cell therapy has emerged as a new tool for several diseases of the nervous system. Stem cells act as minipumps providing trophic and immunomodulatory factors to enhance axonal growth, to modulate the environment, and to reduce neuroinflammation. This capability can be boosted by genetical manipulation to deliver trophic molecules. Different types of stem cells have been tested, according to their properties and the therapeutic aims. They differ from each other for origin, developmental stage, stage of differentiation, and fate lineage. Related to this, stem cells differentiating into neurons could be used for cell replacement, even though the feasibility that stem cells after transplantation in the adult lesioned spinal cord can differentiate into neurons, integrate within neural circuits, and emit axons reaching the muscle is quite remote. The timing of cell therapy has been variable, and may be summarized in the acute and chronic phases of disease, when stem cells interact with a completely different environment. Even though further experimental studies are needed to elucidate the mechanisms of action, the therapeutic, and the side effects of cell therapy, several clinical protocols have been tested or are under trial. Here, we report the state-of-the-art of cell therapy in SCI, in terms of feasibility, outcome, and side effects.

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Cytokine-induced differentiation of multipotent adult progenitor cells into functional smooth muscle cells

Cited by 164 publications

References 56 publications

Inhibitory role of reactive oxygen species in the differentiation of multipotent vascular stem cells into vascular smooth muscle cells in rats: a novel aspect of traditional culture of rat aortic smooth muscle cells

Inhibitory role of reactive oxygen species in the differentiation of multipotent vascular stem cells into vascular smooth muscle cells in rats: a novel aspect of traditional culture of rat aortic smooth muscle cells

Induction of endometrial mesenchymal stem cells into tissue-forming cells suitable for fascial repair

Recent therapeutic strategies for spinal cord injury treatment: possible role of stem cells

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